Difference between revisions of "Team:Manchester/Improve"

 
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<p> The Manchester iGEM 2017 team have improved part <a href="http://partsregistry.org/Part:BBa_K562001"><b>BBa_K562001</b></a>, originally submitted by <a href="https://2011.igem.org/Team:Dundee"></b>Dundee 2011</b></a></p>
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<h2 class="border" style="margin-top: 5vh; text-align: center">Improved Part</h2>
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<p style="color: #f5fcff!important">f</p><br>
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<p style="text-align: center; font-size: 20px!important"><i> Please visit our <a href="https://2017.igem.org/Team:Manchester/Part_Collection"><b>Part Collection</b></a> page for ALL PARTS.</i></p>
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<p style="text-align: center; font-size: 18px!important"> The Manchester iGEM 2017 team have improved part <a href="http://parts.igem.org/Part:BBa_K562001"><b>BBa_K562001</b></a>, originally submitted by team <a href="https://2011.igem.org/Team:Dundee"></b>Dundee 2011</b></a>.</p>
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<p style="text-align: center; font-size: 18px!important">We have removed the illegal XbaI site at 179 in BBa_K562001 to make it biobrick compatible. </p>
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<p style="text-align: center; font-size: 18px!important">We have also further improved this part by adding different Anderson promoters (<a href="http://parts.igem.org/Part:BBa_J23105">Low BBa_J23105</a>, <a href="http://parts.igem.org/Part:BBa_J23108">Medium BBa_J23108</a>, <a href="http://parts.igem.org/Part:BBa_J23104">High BBa_J23104</a>) to control the expression level of the localisation tag.</p>
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<p style="text-align: center; font-size: 18px!important">This enables optimisation of localisation for specific purposes. For example, when targeting a particularly large protein, it may be advantageous to use a lowly expressing promoter to prevent any disturbance to micro-compartment formation.</p>
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<th style="border:1px black solid;background: #d4f4fc;"><b>Part Name</b></th>
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                <th style="border:1px black solid;background: #d4f4fc;"><b>BioBrick</b></th>
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                <th style="border:1px black solid;background: #d4f4fc;"><b>Description</b></th>
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<th style="border:1px black solid;background: #d4f4fc;"><b>Part Type</b></th>
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<td style="border:1px black solid;" class="break">LowPromoter_PduD(1-20)_mCherry</td>
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<td style="border:1px black solid;" class="break"><a href="http://parts.igem.org/Part:BBa_K2213006" target="_blank"><b>BBa_K2213006</b></a></td>
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<td style="border:1px black solid;"><a href="http://parts.igem.org/Part:BBa_K562001" target="_blank"><b>BBa_K562001</b></a>,with the illegal XbaI site removed, and with a low promoter (<a href="http://parts.igem.org/Part:BBa_J23105" target="_blank"><b>BBa_J23105</b></a>), and mCherry added.</td>
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<td style="border:1px black solid;">Basic - Improved</td>
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<td style="border:1px black solid;" class="break">MediumPromoter_PduD(1-20)_mCherry</td>
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<td style="border:1px black solid;" class="break"><a href="http://parts.igem.org/Part:BBa_K2213007"target="_blank"><b>BBa_K2213007</b></a></td>
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<td style="border:1px black solid;"><a href="http://parts.igem.org/Part:BBa_K562001" target="_blank"><b>BBa_K562001</b></a>, with the illegal XbaI site removed, and with a medium promoter (<a href="http://parts.igem.org/Part:BBa_J23108" target="_blank"><b>BBa_J23108</b></a>), and mCherry added.</td>
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<td style="border:1px black solid;">Basic - Improved</td>
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<td style="border:1px black solid;" class="break">HighPromoter_PduD(1-20)_mCherry</td>
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<td style="border:1px black solid;" class="break"><a href="http://parts.igem.org/Part:BBa_K2213008"target="_blank"><b>BBa_K2213008</b></a></td>
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<td style="border:1px black solid;"><a href="http://parts.igem.org/Part:BBa_K562001" target="_blank"><b>BBa_K562001</b></a>, with the illegal XbaI site removed, and with a high promoter (<a href="http://parts.igem.org/Part:BBa_J23104" target="_blank"><b>BBa_J23104</b></a>), and mCherry added.</td>
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<td style="border:1px black solid;">Basic - Improved</td>
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<a class="floatleft1 project projectlink" href= " https://2017.igem.org/Team:Manchester/Part_Collection " ><img src="https://static.igem.org/mediawiki/2017/3/3c/T--Manchester--NEXTARROWPARTS17.jpg"></a>
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<a class="floatright1 project projectlink" href= " https://2017.igem.org/Team:Manchester/Composite_Part " ><img src="https://static.igem.org/mediawiki/2017/1/11/T--Manchester--BACKARROWPARTSMAN17.jpg"></a>
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Latest revision as of 21:07, 1 November 2017

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JUDGING FORM

Improved Part



f


Please visit our Part Collection page for ALL PARTS.


The Manchester iGEM 2017 team have improved part BBa_K562001, originally submitted by team Dundee 2011.

We have removed the illegal XbaI site at 179 in BBa_K562001 to make it biobrick compatible.

We have also further improved this part by adding different Anderson promoters (Low BBa_J23105, Medium BBa_J23108, High BBa_J23104) to control the expression level of the localisation tag.

This enables optimisation of localisation for specific purposes. For example, when targeting a particularly large protein, it may be advantageous to use a lowly expressing promoter to prevent any disturbance to micro-compartment formation.


Part Name BioBrick Description Part Type
LowPromoter_PduD(1-20)_mCherry BBa_K2213006 BBa_K562001,with the illegal XbaI site removed, and with a low promoter (BBa_J23105), and mCherry added. Basic - Improved
MediumPromoter_PduD(1-20)_mCherry BBa_K2213007 BBa_K562001, with the illegal XbaI site removed, and with a medium promoter (BBa_J23108), and mCherry added. Basic - Improved
HighPromoter_PduD(1-20)_mCherry BBa_K2213008 BBa_K562001, with the illegal XbaI site removed, and with a high promoter (BBa_J23104), and mCherry added. Basic - Improved