Difference between revisions of "Team:Manchester/Improve"

We have also further improved this part by adding different Anderson promoters (Low BBa_J23105, Medium BBa_J23108, High BBa_J23104) to control the expression level of the localisation tag.

This enables optimisation of localisation for specific purposes. For example, when targeting a particularly large protein, it may be advantageous to use a lowly expressing promoter to prevent any disturbance to micro-compartment formation.

Part Name	BioBrick	Description	Part Type
LowPromoter_PduD(1-20)_mCherry	BBa_K2213006	BBa_K562001,with the illegal XbaI site removed, and with a low promoter (BBa_J23105), and mCherry added.	Basic - Improved
MediumPromoter_PduD(1-20)_mCherry	BBa_K2213007	BBa_K562001, with the illegal XbaI site removed, and with a medium promoter (BBa_J23108), and mCherry added.	Basic - Improved
HighPromoter_PduD(1-20)_mCherry	BBa_K2213008	BBa_K562001, with the illegal XbaI site removed, and with a high promoter (BBa_J23104), and mCherry added.	Basic - Improved

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+<div class="plan" style="padding: 0px 8vw; background-color: #f5fcff!important">
+<a class="floatleft1 project projectlink" href= " https://2017.igem.org/Team:Manchester/Part_Collection " ><img src="https://static.igem.org/mediawiki/2017/3/3c/T--Manchester--NEXTARROWPARTS17.jpg"></a>
+<a class="floatright1 project projectlink" href= " https://2017.igem.org/Team:Manchester/Composite_Part " ><img src="https://static.igem.org/mediawiki/2017/1/11/T--Manchester--BACKARROWPARTSMAN17.jpg"></a>
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