Difference between revisions of "Team:TECHNION-ISRAEL/Demonstrate"

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<title>Assay</title>
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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<h1>Demonstrate</h1>
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<h3>Gold Medal Criterion #4</h3>
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Teams that can show their system working under real world conditions are usually good at impressing the judges in iGEM. To achieve gold medal criterion #4, convince the judges that your project works. There are many ways in which your project working could be demonstrated, so there is more than one way to meet this requirement. This gold medal criterion was introduced in 2016, so check our what 2016 teams did to achieve a their gold medals!
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Please see the <a href="https://2017.igem.org/Judging/Medals">2017 Medals Page</a> for more information.
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<img src="https://static.igem.org/mediawiki/2017/1/1f/T--TECHNION-ISRAEL--Tolerance_assay_header.png" class="cover" alt=""  style= "width:32% ; margin: auto;">
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<h4> What should we do for our demonstration?</h4>
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<h5> Standard teams </h5>
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If you have built a proof of concept system, you can demonstrate it working under real world conditions. If you have built a biological device that is intended to be a sensor, can you show it detecting whatever it is intended to sense. If it is intended to work in the field, you can show how this might work using a simulated version in the lab, or a simulation of your device in the field.<strong> Please note biological materials must not be taken out of the lab</strong>.
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In an attempt to show proof of concept we set out to test our system under realistic conditions. Ideally we would have tested our constructs <i>in-vivo</i> for tolerance
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induction<sup id = "cite ref-1 " class ="reference">
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<a href="#ref1" original-title>[1]</a>
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</sup>.
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This, unfortunately, was not possible within the framework of iGEM, as animal experimentation is generally discouraged, and the time frame did not allow for an animal experimentation ethics permit.
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In light of these considerations, our team has worked very hard to develop an effective and novel <i>in-vitro</i> assay for tolerance induction.  
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<img class="no-rad" src="https://static.igem.org/mediawiki/2017/e/ed/T--TECHNION-ISRAEL--motekush.png" alt = "" style= "width: 125px; float:right;">
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<h5> Special track teams </h5>
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Special track teams can achieve this medal criterion by bringing their work to the Jamboree and showcasing it in the track event. Art & Design, Measurement, Hardware and Software tracks will all have showcase events at the Giant Jamboree.<strong> Please note biological materials must not be taken out of the lab</strong>.
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<a href="https://2017.igem.org/Team:TECHNION-ISRAEL/Bcell_assay">
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<img src="https://static.igem.org/mediawiki/2017/f/fe/T--TECHNION-ISRAEL--introduction_T_assay.png" class="no-rad" alt=""  style= "width: 86%; margin: auto;">
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<li id="ref1">Baranyi, Ulrike, et al. "Tolerization of a type I allergic immune response through transplantation of genetically modified hematopoietic stem cells."  <i>The Journal of Immunology  </i>180.12 (2008): 8168-8175.</li>
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<a id="back-to-top" href="#" class="btn btn-lg back-to-top" role="button"><img src="https://static.igem.org/mediawiki/2017/f/f9/T--TECHNION-ISRAEL--newUpAB.png" alt=""></a>
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{{:Team:TECHNION-ISRAEL/sponsors}}

Latest revision as of 01:40, 2 November 2017

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Assay






In an attempt to show proof of concept we set out to test our system under realistic conditions. Ideally we would have tested our constructs in-vivo for tolerance induction [1] . This, unfortunately, was not possible within the framework of iGEM, as animal experimentation is generally discouraged, and the time frame did not allow for an animal experimentation ethics permit.

In light of these considerations, our team has worked very hard to develop an effective and novel in-vitro assay for tolerance induction.









  1. Baranyi, Ulrike, et al. "Tolerization of a type I allergic immune response through transplantation of genetically modified hematopoietic stem cells." The Journal of Immunology 180.12 (2008): 8168-8175.
My First Website

Department of Biotechnology & Food Engineering
Technion – Israel Institute of Technology
Haifa 32000, Israel

    • igem.technion.2017@gmail.com