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+ | {{:Team:TECHNION-ISRAEL/navbar}} | ||
+ | <html lang="en"> | ||
+ | <head> | ||
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+ | <title>part improvements</title> | ||
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+ | </head> | ||
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+ | <body> | ||
+ | <br> | ||
+ | <div class = "container-fluid"> | ||
+ | <div class= "row"> | ||
+ | |||
+ | |||
+ | <h1> Part Improvements </h1> | ||
+ | |||
+ | <img src="https://static.igem.org/mediawiki/2017/e/e1/T--TECHNION-ISRAEL--improve_part.png" class="cover" alt="" style= "width:18% ; margin: auto;"> | ||
+ | |||
+ | <br> | ||
+ | <br> | ||
+ | <div class = "col-md-offset-2 col-md-8" > | ||
+ | <div class="row"> | ||
+ | <div class = "col-md-12" > | ||
+ | |||
+ | |||
+ | <br> | ||
+ | |||
+ | <p> | ||
+ | We have improved two existing parts in an attempt to increase their utility to the iGEM community. <br> | ||
+ | We improved the existing part (<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1119008">BBa_K1119008</a>) - a device containing the GFP reporter gene under the CMV promoter with an hGH poly A terminator. By replacing the CMV promoter with TRE promoter (<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520006">BBa_K2520006</a>) we have created a convenient and important positive control for TRE based inducible systems. | ||
+ | <br> | ||
+ | <br> | ||
+ | Additionally, in our project we used the existing tTA transactivator part (<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1061015">BBa_K1061015</a>) to test our <a target="_blank" href="https://2017.igem.org/Team:TECHNION-ISRAEL/delay_mechanism" >Delay Mechanism</a>. To use this basic part, we had to clone it into a mammalian expression vector, requiring time and effort. <br>In order to save future teams time, we have improved this part (<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520009">BBa_K2520009</a>) by adding a promoter (CMV) and a terminator (hGH poly A). Additionally, we tested this part and characterized it. | ||
+ | |||
+ | |||
+ | |||
+ | <br> | ||
+ | <br> | ||
+ | <br> | ||
+ | <br> | ||
+ | |||
+ | |||
+ | |||
+ | <br> | ||
+ | |||
+ | |||
+ | <table class="table table-list-search"> | ||
+ | <thead> | ||
+ | <tr> | ||
+ | <th> </th> | ||
+ | <th> </th> | ||
+ | <th> </th> | ||
+ | <th>Name</th> | ||
+ | <th>Type</th> | ||
+ | <th>Description</th> | ||
+ | <th>Designer</th> | ||
+ | <th>Length</th> | ||
+ | </tr> | ||
+ | </thead> | ||
+ | <tbody> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | <td> | ||
+ | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520006" target="_blank">BBa_K2520006</a> | ||
+ | </td> | ||
+ | <td>Device</td> | ||
+ | <td>TRE-GFP-hGH</td> | ||
+ | <td>Noa Eden, Dana Kadosh, Maya Engal, Shir Ovadia</td> | ||
+ | <td>1532</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | <td> | ||
+ | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520009" target="_blank">BBa_K2520009</a> | ||
+ | </td> | ||
+ | <td>Device</td> | ||
+ | <td>CMV-tTA-hGH</td> | ||
+ | <td>Noa Eden, Dana Kadosh, Maya Engal, Shir Ovadia</td> | ||
+ | <td>1860</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | |||
+ | </tbody> | ||
+ | </table> | ||
+ | |||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | </div> | ||
+ | |||
+ | <a id="back-to-top" href="#" class="btn btn-lg back-to-top" role="button"><img src="https://static.igem.org/mediawiki/2017/f/f9/T--TECHNION-ISRAEL--newUpAB.png" alt=""></a> | ||
+ | </body> | ||
+ | </html> | ||
+ | {{:Team:TECHNION-ISRAEL/sponsors}} |
Latest revision as of 02:29, 2 November 2017
Part Improvements
![](https://static.igem.org/mediawiki/2017/e/e1/T--TECHNION-ISRAEL--improve_part.png)
We have improved two existing parts in an attempt to increase their utility to the iGEM community.
We improved the existing part (BBa_K1119008) - a device containing the GFP reporter gene under the CMV promoter with an hGH poly A terminator. By replacing the CMV promoter with TRE promoter (BBa_K2520006) we have created a convenient and important positive control for TRE based inducible systems.
Additionally, in our project we used the existing tTA transactivator part (BBa_K1061015) to test our Delay Mechanism. To use this basic part, we had to clone it into a mammalian expression vector, requiring time and effort.
In order to save future teams time, we have improved this part (BBa_K2520009) by adding a promoter (CMV) and a terminator (hGH poly A). Additionally, we tested this part and characterized it.
Name | Type | Description | Designer | Length | |||
---|---|---|---|---|---|---|---|
BBa_K2520006 | Device | TRE-GFP-hGH | Noa Eden, Dana Kadosh, Maya Engal, Shir Ovadia | 1532 | |||
BBa_K2520009 | Device | CMV-tTA-hGH | Noa Eden, Dana Kadosh, Maya Engal, Shir Ovadia | 1860 |
![](https://static.igem.org/mediawiki/2017/f/f9/T--TECHNION-ISRAEL--newUpAB.png)