Difference between revisions of "Team:TECHNION-ISRAEL/Improve"

 
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<h1> Parts Improvement </h1>
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We have improved two existing parts in an attempt to increase their utility to the iGEM community. <br>
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We improved the existing part (<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1119008">BBa_K1119008</a>) - a device containing the GFP reporter gene under the CMV promoter with an hGH poly A terminator. By replacing the CMV promoter with TRE promoter (<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520006">BBa_K2520006</a>) we have created a convenient and important positive control for TRE based inducible systems.
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Additionally, in our project we used the existing tTA transactivator part (<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1061015">BBa_K1061015</a>)  to test our <a target="_blank" href="https://2017.igem.org/Team:TECHNION-ISRAEL/delay_mechanism" >Delay Mechanism</a>. To use this basic part, we had to clone it into a mammalian expression vector, requiring time and effort. <br>In order to save future teams time, we have improved this part (<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520009">BBa_K2520009</a>) by adding a promoter (CMV) and a terminator (hGH poly A). Additionally, we tested this part and characterized it.
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This is the full list of our parts:
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<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520006" target="_blank">BBa_K2520006</a>
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<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520006" target="_blank">BBa_K2520006</a>
 
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                             <td>Device</td>
 
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                             <td>TRE-GFP-hGH</td>
 
                             <td>TRE-GFP-hGH</td>
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                             <td>Noa Eden, Dana Kadosh, Maya Engal</td>
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                             <td>Noa Eden, Dana Kadosh, Maya Engal, Shir Ovadia</td>
 
<td>1532</td>
 
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<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520009" target="_blank">BBa_K2520009</a>
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<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520009" target="_blank">BBa_K2520009</a>
 
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                             <td>Device</td>
 
                             <td>Device</td>
 
                             <td>CMV-tTA-hGH</td>
 
                             <td>CMV-tTA-hGH</td>
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                             <td>Noa Eden, Dana Kadosh, Maya Engal</td>
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                             <td>Noa Eden, Dana Kadosh, Maya Engal, Shir Ovadia</td>
 
<td>1860</td>
 
<td>1860</td>
 
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Latest revision as of 02:29, 2 November 2017

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part improvements

Part Improvements




We have improved two existing parts in an attempt to increase their utility to the iGEM community.
We improved the existing part (BBa_K1119008) - a device containing the GFP reporter gene under the CMV promoter with an hGH poly A terminator. By replacing the CMV promoter with TRE promoter (BBa_K2520006) we have created a convenient and important positive control for TRE based inducible systems.

Additionally, in our project we used the existing tTA transactivator part (BBa_K1061015) to test our Delay Mechanism. To use this basic part, we had to clone it into a mammalian expression vector, requiring time and effort.
In order to save future teams time, we have improved this part (BBa_K2520009) by adding a promoter (CMV) and a terminator (hGH poly A). Additionally, we tested this part and characterized it.




Name Type Description Designer Length
BBa_K2520006 Device TRE-GFP-hGH Noa Eden, Dana Kadosh, Maya Engal, Shir Ovadia 1532
BBa_K2520009 Device CMV-tTA-hGH Noa Eden, Dana Kadosh, Maya Engal, Shir Ovadia 1860
My First Website

Department of Biotechnology & Food Engineering
Technion – Israel Institute of Technology
Haifa 32000, Israel

    • igem.technion.2017@gmail.com