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{{Team:Lethbridge/assets/reset.css}} | {{Team:Lethbridge/assets/reset.css}} | ||
+ | {{Team:Lethbridge/assets/sidemenuremove.css}} | ||
{{Team:Lethbridge/navbar}} | {{Team:Lethbridge/navbar}} | ||
+ | {{Team:Lethbridge/assets/allpages.css}} | ||
+ | {{Team:Lethbridge/top}} | ||
<html> | <html> | ||
− | |||
<style> | <style> | ||
− | /* | + | /*RESET AND REMOVAL CSS IS PART OF RESET AND SIDEMENUREMOVE TEMPLATE*/ |
− | + | /* RULES FOR ALL PAGES ARE PART OF THE ALLPAGES.CSS TEMPLATE*/ | |
− | # | + | /* RULES FOR THIS PAGE ONLY */ |
− | # | + | .contentDiv {display: inline-block; vertical-align: middle;} |
− | # | + | |
− | # | + | /* PROTOCOL TABLE BEGINS */ |
− | /*# | + | #protocolTable tr {font-size: calc(8px + 0.75vw) !important; line-height: calc(1em + 0.5vw) !important; text-align: center !important; vertical-align: top;} /*width: 800px;*/ |
− | + | #protocolTable td {align-content:center; padding: 15px;} /*width: 400px*/ | |
− | + | #protocolTable tr:nth-child(even) {background-color: white;} | |
− | + | #protocolTable a:link {color: #041f39;} | |
+ | #protocolTable a:active, #protocolTable a:hover {color: #f5af3e;} | ||
+ | #protocolTable a:visited {color: #377b7e;} | ||
+ | /* PROTOCOL TABLE ENDS */ | ||
+ | |||
+ | /* PRODUCT REAGENT TABLE BEGINS */ | ||
+ | #proreaTable td, th {border: 1px solid black; text-align: center; padding: 6px;} | ||
+ | #proreaTable tr td {font-size: calc(8px + 0.5vw) !important; line-height: calc(1em + 0.5vw) !important; text-align: center !important; vertical-align: top;} | ||
+ | #proreaTable tr:nth-child(even) {background-color: white;} | ||
+ | /* PRODUCT REAGENT TABLE ENDS */ | ||
+ | |||
+ | @media only screen and (max-width: 500px) { | ||
+ | #allContent {margin-left: 1%; margin-right: 1%;} | ||
+ | #proreaTable td, th {border: 1px solid black; text-align: center; padding: 4px;} | ||
+ | #proreaTable tr td {font-size: calc(6px + 0.25vw) !important; line-height: calc(1em + 0.5vw) !important; text-align: center !important; vertical-align: top;} | ||
+ | } | ||
</style> | </style> | ||
− | |||
<body> | <body> | ||
− | <div><img src="https://static.igem.org/mediawiki/2017/f/f4/Banner_LAexperiments.png" class="center | + | <div><img src="https://static.igem.org/mediawiki/2017/f/f4/Banner_LAexperiments.png" class="bannerImg"></div> |
+ | <div id="allContent"><!--div to set margin for all content but header and footer image --> | ||
+ | <br> | ||
+ | |||
+ | <div class="segmentDiv"><h2 class="segmentHeader">Protocols</h2></div> | ||
+ | |||
+ | <center> | ||
+ | <div> | ||
+ | |||
+ | <table id="protocolTable"> | ||
+ | <tr> | ||
+ | <td><a href="https://static.igem.org/mediawiki/2017/5/5f/Agarose_Gel_Extraction.pdf" target="_blank">Agarose Gel Extraction</a></td> | ||
+ | <td><a href="https://static.igem.org/mediawiki/2017/6/66/Colony_PCR.pdf" target="_blank">Colony PCR</a></td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><a href="https://static.igem.org/mediawiki/2017/6/6f/DNA_Purification_from_Enzymatic_Reactions.pdf" target="_blank">DNA Purification from Enzymatic Reactions</a></td> | ||
+ | <td><a href="https://static.igem.org/mediawiki/2017/5/51/In_vitro_transcription.pdf" target="_blank"><i>In vitro</i> Transcription</a></td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><a href="https://static.igem.org/mediawiki/2017/b/ba/LB_Media.pdf" target="_blank">LB Media</a></td> | ||
+ | <td><a href="https://static.igem.org/mediawiki/2017/9/9d/Ligation.pdf" target="_blank">Ligations</a></td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><a href="https://static.igem.org/mediawiki/2017/6/68/PCR_Amplification.pdf" target="_blank">PCR Amplification</a></td> | ||
+ | <td><a href="https://static.igem.org/mediawiki/2017/e/e5/PCR_Product_Purification.pdf" target="_blank">PCR Product Purification</a></td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><a href="https://static.igem.org/mediawiki/2017/f/f2/PhenolFChloroform_extraction_%26_ethanol_percipitation.pdf" target="_blank">Phenol/Chloroform Extraction and Ethanol Precipitation</a></td> | ||
+ | <td><a href="https://static.igem.org/mediawiki/2017/e/eb/PJET_Cloning.pdf" target="_blank">pJET Cloning</a></td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><a href="https://static.igem.org/mediawiki/2017/0/08/Plasmid_Miniprep.pdf" target="_blank">Plasmid Miniprep</a></td> | ||
+ | <td><a href="https://static.igem.org/mediawiki/2017/7/78/Preparation_of_Chemical_Competent_Cells.pdf" target="_blank">Preparation of Chemical Competent Cells</a></td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><a href="https://static.igem.org/mediawiki/2017/2/2c/Protein_Overexpression_.pdf" target="_blank">Protein Overexpression</a></td> | ||
+ | <td><a href="https://static.igem.org/mediawiki/2017/5/5f/PURExpress.pdf" target="_blank">PURExpress NEB Kit</a></td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><a href="https://static.igem.org/mediawiki/2017/f/fc/Purification_of_Poly-Histidine_Tagged_Proteins.pdf" target="_blank">Purification of HexaHistidine Tagged Proteins</a></td> | ||
+ | <td><a href="https://static.igem.org/mediawiki/2017/1/10/Restriction_Enzyme_Digest_and_Dephosphorylation.pdf" target="_blank">Restriction Enzyme Digest and Dephosphorylation</a></td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><a href="https://static.igem.org/mediawiki/2017/2/2a/T7-RNA_Polymerase_Purification.pdf" target="_blank">T7- RNA Polymerase Purification</a></td> | ||
+ | <td><a href="https://static.igem.org/mediawiki/2017/a/a6/Transformation.pdf" target="_blank">Transformation</a></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="https://static.igem.org/mediawiki/2017/c/cd/MS2-tRNA_Complex_Purification.pdf" target="_blank">MS2-tRNA Affinity Chromatography Purification</a></td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | </div> | ||
+ | </center> | ||
+ | <br> | ||
+ | |||
+ | <div class="segmentDiv"> | ||
+ | <h2 class="segmentHeader">Products/Reagents</h2> | ||
+ | </div> | ||
+ | |||
+ | <center> | ||
+ | <table id="proreaTable"> | ||
+ | <tr> | ||
+ | <th style="background-color: #377b7e; color: #ffffff; font-weight: bold;">Enzymes</th> | ||
+ | <th style="background-color: #377b7e; color: #ffffff; font-weight: bold;">Buffers/Reagents</th> | ||
+ | <th style="background-color: #377b7e; color: #ffffff; font-weight: bold;">Other Products</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Shrimp Alkaline Phosphatase</td> | ||
+ | <td>Affinity Chromatography Buffers</td> | ||
+ | <td>Ni²+ Affinity Resin</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>DNA Blunting Enzyme</td> | ||
+ | <td>IPTG</td> | ||
+ | <td>Prefix Forward: 5’GAATTCGCGGCCGCTTCTAG3’</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Phusion Polymerase Master Mix</td> | ||
+ | <td>Kanomycin / Chloramphenicol / Ampicillin </td> | ||
+ | <td>Prefix Reverse: 5’CTGCAGCGGCCGCTACTAGTA3’</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>PFU Polymerase</td> | ||
+ | <td>Dimethyl Sulfur Dioxide</td> | ||
+ | <td>Prefix Forward +6:5’GGCTCAGAATTCGCGGCCGCTTCTAGAG3’</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>T7 RNA Polymerase</td> | ||
+ | <td>Competent Cell Buffers</td> | ||
+ | <td>Suffix Reverse +6: 5’CTGGACCTGCAGCGGCCGCTACTAGTA3’</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>T7 DNA Polymerase</td> | ||
+ | <td>T7 RNA Polymerase Purification Buffers</td> | ||
+ | <td>Dialysis Tubing</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>PURExpressKit (New England Biolabs)</td> | ||
+ | <td>Phenol </td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>XbaI (NEB)</td> | ||
+ | <td>Chloroform</td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>SpeI (NEB)</td> | ||
+ | <td>10x PFU Buffer</td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>PstI (NEB)</td> | ||
+ | <td>NTP</td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>EcoRI (NEB)</td> | ||
+ | <td>GMP</td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Dream Taq DNA Polymerase</td> | ||
+ | <td>10x Taq Buffer</td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Taq DNA Polymerase</td> | ||
+ | <td>Ligase Buffer</td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>RNase A</td> | ||
+ | <td>SDS-PAGE Reagents</td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>TraB</td> | ||
+ | <td>Urea PAGE reagents</td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>iPPase</td> | ||
+ | <td>Agarose</td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>RNase Inhibitor</td> | ||
+ | <td>Agar</td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>DNaseI</td> | ||
+ | <td>Tryptone</td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td></td> | ||
+ | <td>Yeast Extract</td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td></td> | ||
+ | <td>TAKMz</td> | ||
+ | <td> </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td></td> | ||
+ | <td>BioBasic Plasmid DNA miniprep Kit</td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td></td> | ||
+ | <td>Imidazole</td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | </center> | ||
+ | |||
+ | <br> | ||
+ | <br> | ||
+ | </div><!-- closing #allContent --> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/7/7d/Banner_footer_blank.png" class="bannerImg"> | ||
</body> | </body> | ||
</html> | </html> |
Latest revision as of 03:07, 2 November 2017
Protocols
Products/Reagents
Enzymes | Buffers/Reagents | Other Products |
---|---|---|
Shrimp Alkaline Phosphatase | Affinity Chromatography Buffers | Ni²+ Affinity Resin |
DNA Blunting Enzyme | IPTG | Prefix Forward: 5’GAATTCGCGGCCGCTTCTAG3’ |
Phusion Polymerase Master Mix | Kanomycin / Chloramphenicol / Ampicillin | Prefix Reverse: 5’CTGCAGCGGCCGCTACTAGTA3’ |
PFU Polymerase | Dimethyl Sulfur Dioxide | Prefix Forward +6:5’GGCTCAGAATTCGCGGCCGCTTCTAGAG3’ |
T7 RNA Polymerase | Competent Cell Buffers | Suffix Reverse +6: 5’CTGGACCTGCAGCGGCCGCTACTAGTA3’ |
T7 DNA Polymerase | T7 RNA Polymerase Purification Buffers | Dialysis Tubing |
PURExpressKit (New England Biolabs) | Phenol | |
XbaI (NEB) | Chloroform | |
SpeI (NEB) | 10x PFU Buffer | |
PstI (NEB) | NTP | |
EcoRI (NEB) | GMP | |
Dream Taq DNA Polymerase | 10x Taq Buffer | |
Taq DNA Polymerase | Ligase Buffer | |
RNase A | SDS-PAGE Reagents | |
TraB | Urea PAGE reagents | |
iPPase | Agarose | |
RNase Inhibitor | Agar | |
DNaseI | Tryptone | |
Yeast Extract | ||
TAKMz | ||
BioBasic Plasmid DNA miniprep Kit | ||
Imidazole |