Difference between revisions of "Team:Lethbridge/Experiments"

 
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<div><img src="https://static.igem.org/mediawiki/2017/f/f4/Banner_LAexperiments.png"  class="center fit"></div>
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<div><img src="https://static.igem.org/mediawiki/2017/f/f4/Banner_LAexperiments.png"  class="bannerImg"></div>
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<p><a href=protocol">Agarose Gel Extraction</a></p>
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<div id="allContent"><!--div to set margin for all content but header and footer image -->
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<br>
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<div class="segmentDiv"><h2 class="segmentHeader">Protocols</h2></div>
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<center>
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    <div>
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    <table id="protocolTable">
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    <tr>
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        <td><a href="https://static.igem.org/mediawiki/2017/5/5f/Agarose_Gel_Extraction.pdf" target="_blank">Agarose Gel Extraction</a></td>
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        <td><a href="https://static.igem.org/mediawiki/2017/6/66/Colony_PCR.pdf" target="_blank">Colony PCR</a></td>
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    </tr>
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 +
    <tr>
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        <td><a href="https://static.igem.org/mediawiki/2017/6/6f/DNA_Purification_from_Enzymatic_Reactions.pdf" target="_blank">DNA Purification from Enzymatic Reactions</a></td>
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        <td><a href="https://static.igem.org/mediawiki/2017/5/51/In_vitro_transcription.pdf" target="_blank"><i>In vitro</i> Transcription</a></td>
 +
    </tr>
 +
   
 +
    <tr>
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        <td><a href="https://static.igem.org/mediawiki/2017/b/ba/LB_Media.pdf" target="_blank">LB Media</a></td>
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        <td><a href="https://static.igem.org/mediawiki/2017/9/9d/Ligation.pdf" target="_blank">Ligations</a></td>
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    </tr>
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 +
    <tr>
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        <td><a href="https://static.igem.org/mediawiki/2017/6/68/PCR_Amplification.pdf" target="_blank">PCR Amplification</a></td>
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        <td><a href="https://static.igem.org/mediawiki/2017/e/e5/PCR_Product_Purification.pdf" target="_blank">PCR Product Purification</a></td>
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    </tr>
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 +
    <tr>
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        <td><a href="https://static.igem.org/mediawiki/2017/f/f2/PhenolFChloroform_extraction_%26_ethanol_percipitation.pdf" target="_blank">Phenol/Chloroform Extraction and Ethanol Precipitation</a></td>
 +
        <td><a href="https://static.igem.org/mediawiki/2017/e/eb/PJET_Cloning.pdf" target="_blank">pJET Cloning</a></td>
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    </tr>
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 +
    <tr>
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        <td><a href="https://static.igem.org/mediawiki/2017/0/08/Plasmid_Miniprep.pdf" target="_blank">Plasmid Miniprep</a></td>
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        <td><a href="https://static.igem.org/mediawiki/2017/7/78/Preparation_of_Chemical_Competent_Cells.pdf" target="_blank">Preparation of Chemical Competent Cells</a></td>
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    </tr>
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    <tr>
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        <td><a href="https://static.igem.org/mediawiki/2017/2/2c/Protein_Overexpression_.pdf" target="_blank">Protein Overexpression</a></td>
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        <td><a href="https://static.igem.org/mediawiki/2017/5/5f/PURExpress.pdf" target="_blank">PURExpress NEB Kit</a></td>
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    </tr>
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 +
    <tr>
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        <td><a href="https://static.igem.org/mediawiki/2017/f/fc/Purification_of_Poly-Histidine_Tagged_Proteins.pdf" target="_blank">Purification of HexaHistidine Tagged Proteins</a></td>
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        <td><a href="https://static.igem.org/mediawiki/2017/1/10/Restriction_Enzyme_Digest_and_Dephosphorylation.pdf" target="_blank">Restriction Enzyme Digest and Dephosphorylation</a></td>
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    </tr>
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 +
    <tr>
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        <td><a href="https://static.igem.org/mediawiki/2017/2/2a/T7-RNA_Polymerase_Purification.pdf" target="_blank">T7- RNA Polymerase Purification</a></td>
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        <td><a href="https://static.igem.org/mediawiki/2017/a/a6/Transformation.pdf" target="_blank">Transformation</a></td>
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    </tr>
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    <tr>
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        <td><a href="https://static.igem.org/mediawiki/2017/c/cd/MS2-tRNA_Complex_Purification.pdf" target="_blank">MS2-tRNA Affinity Chromatography Purification</a></td>
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        <td></td>
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    </tr>
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</table>
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</div>
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<br>
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<div class="segmentDiv">
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<h2 class="segmentHeader">Products/Reagents</h2>
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<table id="proreaTable">
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  <tr>
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    <th style="background-color: #377b7e; color: #ffffff; font-weight: bold;">Enzymes</th>
 +
    <th style="background-color: #377b7e; color: #ffffff; font-weight: bold;">Buffers/Reagents</th>
 +
    <th style="background-color: #377b7e; color: #ffffff; font-weight: bold;">Other Products</th>
 +
  </tr>
 +
  <tr>
 +
    <td>Shrimp Alkaline Phosphatase</td>
 +
    <td>Affinity Chromatography Buffers</td>
 +
  <td>Ni²+ Affinity Resin</td>
 +
  </tr>
 +
  <tr>
 +
    <td>DNA Blunting Enzyme</td>
 +
    <td>IPTG</td>
 +
  <td>Prefix Forward: 5’GAATTCGCGGCCGCTTCTAG3’</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Phusion Polymerase Master Mix</td>
 +
    <td>Kanomycin / Chloramphenicol / Ampicillin </td>
 +
  <td>Prefix Reverse: 5’CTGCAGCGGCCGCTACTAGTA3’</td>
 +
  </tr>
 +
  <tr>
 +
    <td>PFU Polymerase</td>
 +
    <td>Dimethyl Sulfur Dioxide</td>
 +
  <td>Prefix Forward +6:5’GGCTCAGAATTCGCGGCCGCTTCTAGAG3’</td>
 +
  </tr>
 +
  <tr>
 +
    <td>T7 RNA Polymerase</td>
 +
    <td>Competent Cell Buffers</td>
 +
  <td>Suffix Reverse +6: 5’CTGGACCTGCAGCGGCCGCTACTAGTA3’</td>
 +
  </tr>
 +
  <tr>
 +
    <td>T7 DNA Polymerase</td>
 +
    <td>T7 RNA Polymerase Purification Buffers</td>
 +
    <td>Dialysis Tubing</td>
 +
  </tr>
 +
  <tr>
 +
    <td>PURExpressKit (New England Biolabs)</td>
 +
    <td>Phenol </td>
 +
  <td></td>
 +
  </tr>
 +
  <tr>
 +
    <td>XbaI (NEB)</td>
 +
    <td>Chloroform</td>
 +
  <td></td>
 +
  </tr>
 +
<tr>
 +
    <td>SpeI (NEB)</td>
 +
    <td>10x PFU Buffer</td>
 +
    <td></td>
 +
</tr>
 +
<tr>
 +
    <td>PstI (NEB)</td>
 +
    <td>NTP</td>
 +
    <td></td>
 +
</tr>
 +
<tr>
 +
      <td>EcoRI (NEB)</td>
 +
      <td>GMP</td>
 +
    <td></td>
 +
</tr>
 +
<tr>
 +
      <td>Dream Taq DNA Polymerase</td>
 +
      <td>10x Taq Buffer</td>
 +
      <td></td>
 +
</tr>
 +
<tr>
 +
      <td>Taq DNA Polymerase</td>
 +
    <td>Ligase Buffer</td>
 +
    <td></td>
 +
</tr>
 +
<tr>
 +
      <td>RNase A</td>
 +
      <td>SDS-PAGE Reagents</td>
 +
    <td></td>
 +
</tr>
 +
<tr>
 +
      <td>TraB</td>
 +
      <td>Urea PAGE reagents</td>
 +
      <td></td>
 +
</tr>
 +
<tr>
 +
      <td>iPPase</td>
 +
    <td>Agarose</td>
 +
    <td></td>
 +
</tr>
 +
<tr>
 +
    <td>RNase Inhibitor</td>
 +
    <td>Agar</td>
 +
    <td></td>
 +
</tr>
 +
<tr>
 +
    <td>DNaseI</td>
 +
    <td>Tryptone</td>
 +
    <td></td>
 +
</tr>
 +
<tr>
 +
    <td></td>
 +
    <td>Yeast Extract</td>
 +
    <td></td>
 +
</tr>
 +
<tr>
 +
    <td></td>
 +
    <td>TAKMz</td>
 +
    <td> </td>
 +
</tr>
 +
<tr>
 +
    <td></td>
 +
    <td>BioBasic Plasmid DNA miniprep Kit</td>
 +
    <td></td>
 +
</tr>
 +
<tr>
 +
    <td></td>
 +
    <td>Imidazole</td>
 +
    <td></td>
 +
</tr>
 +
</table>
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</center>
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Latest revision as of 03:07, 2 November 2017

top

Protocols


Products/Reagents

Enzymes Buffers/Reagents Other Products
Shrimp Alkaline Phosphatase Affinity Chromatography Buffers Ni²+ Affinity Resin
DNA Blunting Enzyme IPTG Prefix Forward: 5’GAATTCGCGGCCGCTTCTAG3’
Phusion Polymerase Master Mix Kanomycin / Chloramphenicol / Ampicillin Prefix Reverse: 5’CTGCAGCGGCCGCTACTAGTA3’
PFU Polymerase Dimethyl Sulfur Dioxide Prefix Forward +6:5’GGCTCAGAATTCGCGGCCGCTTCTAGAG3’
T7 RNA Polymerase Competent Cell Buffers Suffix Reverse +6: 5’CTGGACCTGCAGCGGCCGCTACTAGTA3’
T7 DNA Polymerase T7 RNA Polymerase Purification Buffers Dialysis Tubing
PURExpressKit (New England Biolabs) Phenol
XbaI (NEB) Chloroform
SpeI (NEB) 10x PFU Buffer
PstI (NEB) NTP
EcoRI (NEB) GMP
Dream Taq DNA Polymerase 10x Taq Buffer
Taq DNA Polymerase Ligase Buffer
RNase A SDS-PAGE Reagents
TraB Urea PAGE reagents
iPPase Agarose
RNase Inhibitor Agar
DNaseI Tryptone
Yeast Extract
TAKMz
BioBasic Plasmid DNA miniprep Kit
Imidazole