Difference between revisions of "Team:Lethbridge/Experiments"

 
(53 intermediate revisions by 4 users not shown)
Line 1: Line 1:
 
{{Team:Lethbridge/assets/reset.css}}
 
{{Team:Lethbridge/assets/reset.css}}
 +
{{Team:Lethbridge/assets/sidemenuremove.css}}
 
{{Team:Lethbridge/navbar}}
 
{{Team:Lethbridge/navbar}}
 +
{{Team:Lethbridge/assets/allpages.css}}
 +
{{Team:Lethbridge/top}}
 
<html>
 
<html>
−
<head>
 
 
<style>
 
<style>
−
/*SIDE MENU FOR IGEM STUFF*/
+
/*RESET AND REMOVAL CSS IS PART OF RESET AND SIDEMENUREMOVE TEMPLATE*/
−
#sideMenu{width: 200px;position: absolute;top: 20px;left: 1020px;z-index: 10;padding-top: 0px;padding-bottom: 15px;padding-left: 15px;padding-right: 15px;background-color: white;text-align: left;display: none;z-index: 9996;}
+
/* RULES FOR ALL PAGES ARE PART OF THE ALLPAGES.CSS TEMPLATE*/
−
#content{width: 100%;padding: 0px;margin-left: 0px;}
+
/* RULES FOR THIS PAGE ONLY */
−
#top_title{overflow: hidden;display: none;}
+
−
#top_menu_14{height: 20px;}
+
−
#top_menu_under{height: 0px;}
+
−
/*#globalWrapper{padding-bottom: 200px;}*/
+
−
 
+
−
/*VISIBLE CONTENT STYLING BEGINS HERE*/
+
−
body {padding-top: 3.89em; font-family: Arial, sans-serif; background-color:#F2F2F2; color: black !important;}
+
−
h1, h2 {text-align: center; color: black !important}
+
−
h3 {font-style: oblique;}
+
−
.userWrapper {margin-left: 7%; margin-right: 7%;}
+
−
.segmentHeader {font-size: 4vw; overflow: hidden; text-align: center;}
+
−
.segmentHeader:before, .segmentHeader:after {background-color: black; content: ""; display: inline-block; height: 3px; position: relative; vertical-align: middle; width: 50%;}
+
−
.segmentHeader:before {margin-left: -50%; right- 0.5 em;}
+
−
.segmentHeader:after {left: 0.5em; margin-right: -50%;}
+
−
.segmentDiv {margin-left: 5% !important; margin-right: 5% !important;}
+
−
.pageText {font-size: calc(12px + 0.5vw) !important; line-height: calc(0.25em + 0.5vw) !important; text-align: left !important; width:99%; font-weight: normal; padding: 5px;}
+
−
 
+
−
.segmentContainer p.segment2 {font-size: calc(22px + 0.5vw) !important; line-height: calc(1.1em + 0.5vw) !important;}
+
 
.contentDiv {display: inline-block; vertical-align: middle;}
 
.contentDiv {display: inline-block; vertical-align: middle;}
  
−
#protocolList {margin-left: 15%;}
+
/* PROTOCOL TABLE BEGINS */
−
a:link {color: #041f39; background-color: transparent; text-decoration: underline;}
+
#protocolTable tr {font-size: calc(8px + 0.75vw) !important; line-height: calc(1em + 0.5vw) !important; text-align: center !important; vertical-align: top;} /*width: 800px;*/
−
a:visited {color: #377b7e; background-color: transparent; text-decoration: underline;}
+
#protocolTable td {align-content:center; padding: 15px;} /*width: 400px*/
−
a:hover {color: #f5af3e; background-color: transparent; text-decoration: underline;}
+
#protocolTable tr:nth-child(even) {background-color: white;}
−
a:active {color: #f5af3e; background-color: transparent; text-decoration: underline;}
+
#protocolTable a:link {color: #041f39;}
 +
#protocolTable a:active, #protocolTable a:hover {color: #f5af3e;}
 +
#protocolTable a:visited {color: #377b7e;}
 +
/* PROTOCOL TABLE ENDS */
  
−
#proreaTable td, th {border: 1px solid black; text-align: center; padding: 8px;}
+
/* PRODUCT REAGENT TABLE BEGINS */
 +
#proreaTable td, th {border: 1px solid black; text-align: center; padding: 6px;}
 +
#proreaTable tr td {font-size: calc(8px + 0.5vw) !important; line-height: calc(1em + 0.5vw) !important; text-align: center !important; vertical-align: top;}
 
#proreaTable tr:nth-child(even) {background-color: white;}
 
#proreaTable tr:nth-child(even) {background-color: white;}
 +
/* PRODUCT REAGENT TABLE ENDS */
 +
 +
@media only screen and (max-width: 500px) {
 +
    #allContent {margin-left: 1%; margin-right: 1%;}
 +
    #proreaTable td, th {border: 1px solid black; text-align: center; padding: 4px;}
 +
    #proreaTable tr td {font-size: calc(6px + 0.25vw) !important; line-height: calc(1em + 0.5vw) !important; text-align: center !important; vertical-align: top;}
 +
    }
 
</style>
 
</style>
−
</head>
 
 
<body>
 
<body>
−
<div><img src="https://static.igem.org/mediawiki/2017/f/f4/Banner_LAexperiments.png"  class="center fit"></div>
+
<div><img src="https://static.igem.org/mediawiki/2017/f/f4/Banner_LAexperiments.png"  class="bannerImg"></div>
 +
<div id="allContent"><!--div to set margin for all content but header and footer image -->
 
<br>
 
<br>
  
−
<div class="segmentDiv">
+
<div class="segmentDiv"><h2 class="segmentHeader">Protocols</h2></div>
−
<h2 class="segmentHeader">Protocols</h2>
+
−
</div>
+
  
−
<div id="protocolList">
+
<center>
−
<p class="pageText"><a href="https://static.igem.org/mediawiki/2017/b/ba/LB_Media.pdf">LB Media</a></p>  
+
    <div>
−
<p class="pageText"><a href="https://static.igem.org/mediawiki/2017/5/5f/Agarose_Gel_Extraction.pdf">Agarose Gel Extraction</a></p>  
+
 
−
<p class="pageText"><a href="https://static.igem.org/mediawiki/2017/6/68/PCR_Amplification.pdf">PCR Amplification</a></p>  
+
    <table id="protocolTable">
−
<p class="pageText"><a href="https://static.igem.org/mediawiki/2017/6/66/Colony_PCR.pdf">Colony PCR</a></p>  
+
    <tr>
−
<p class="pageText"><a href="https://static.igem.org/mediawiki/2017/6/6f/DNA_Purification_from_Enzymatic_Reactions.pdf">DNA PUrification from Enzymatic Reactions</a></p>  
+
        <td><a href="https://static.igem.org/mediawiki/2017/5/5f/Agarose_Gel_Extraction.pdf" target="_blank">Agarose Gel Extraction</a></td>
−
<p class="pageText"><a href="https://static.igem.org/mediawiki/2017/e/e5/PCR_Product_Purification.pdf">PCR product Purification</a></p>  
+
        <td><a href="https://static.igem.org/mediawiki/2017/6/66/Colony_PCR.pdf" target="_blank">Colony PCR</a></td>
−
<p class="pageText"><a href="https://static.igem.org/mediawiki/2017/5/51/In_vitro_transcription.pdf">In Vitro Transcription</a></p>  
+
    </tr>
−
<p class="pageText"><a href="https://static.igem.org/mediawiki/2017/6/6c/Ligation_protocol.pdf">Ligations</a></p>  
+
   
−
<p class="pageText"><a href="https://static.igem.org/mediawiki/2017/d/df/PhenolChloroform_extraction_%26_ethanol_percipitation.pdf">Phenol/Chloroform Extraction and Ethanol Precipitation</a></p>  
+
    <tr>
−
<p class="pageText"><a href="https://static.igem.org/mediawiki/2017/e/eb/PJET_Cloning.pdf">pJET Cloning</a></p>  
+
        <td><a href="https://static.igem.org/mediawiki/2017/6/6f/DNA_Purification_from_Enzymatic_Reactions.pdf" target="_blank">DNA Purification from Enzymatic Reactions</a></td>
−
<p class="pageText"><a href="https://static.igem.org/mediawiki/2017/0/08/Plasmid_Miniprep.pdf">Plasmid Miniprep</a></p>  
+
        <td><a href="https://static.igem.org/mediawiki/2017/5/51/In_vitro_transcription.pdf" target="_blank"><i>In vitro</i> Transcription</a></td>
−
<p class="pageText"><a href="https://static.igem.org/mediawiki/2017/7/78/Preparation_of_Chemical_Competent_Cells.pdf">Preparation of Chemical Competent Cells</a></p>  
+
    </tr>
−
<p class="pageText"><a href="https://static.igem.org/mediawiki/2017/2/2c/Protein_Overexpression_.pdf">Protein Overexpression</a></p>  
+
   
−
<p class="pageText"><a href="https://static.igem.org/mediawiki/2017/5/5f/PURExpress.pdf">PURExpress NEB Kit</a></p>  
+
    <tr>
−
<p class="pageText"><a href="https://static.igem.org/mediawiki/2017/f/fc/Purification_of_Poly-Histidine_Tagged_Proteins.pdf">Purification of Poly-Histidine Tagged Proteins</a></p>  
+
        <td><a href="https://static.igem.org/mediawiki/2017/b/ba/LB_Media.pdf" target="_blank">LB Media</a></td>
−
<p class="pageText"><a href="https://static.igem.org/mediawiki/2017/1/10/Restriction_Enzyme_Digest_and_Dephosphorylation.pdf">Restriction Enzyme Digest and Dephosphorylation</a></p>  
+
        <td><a href="https://static.igem.org/mediawiki/2017/9/9d/Ligation.pdf" target="_blank">Ligations</a></td>
−
<p class="pageText"><a href="https://static.igem.org/mediawiki/2017/2/2a/T7-RNA_Polymerase_Purification.pdf">T7- RNA Polymerase Purification</a></p>  
+
    </tr>
−
<p class="pageText"><a href="https://static.igem.org/mediawiki/2017/a/a6/Transformation.pdf">Transformation</a></p>
+
   
 +
    <tr>
 +
        <td><a href="https://static.igem.org/mediawiki/2017/6/68/PCR_Amplification.pdf" target="_blank">PCR Amplification</a></td>
 +
        <td><a href="https://static.igem.org/mediawiki/2017/e/e5/PCR_Product_Purification.pdf" target="_blank">PCR Product Purification</a></td>
 +
    </tr>
 +
   
 +
    <tr>
 +
        <td><a href="https://static.igem.org/mediawiki/2017/f/f2/PhenolFChloroform_extraction_%26_ethanol_percipitation.pdf" target="_blank">Phenol/Chloroform Extraction and Ethanol Precipitation</a></td>
 +
        <td><a href="https://static.igem.org/mediawiki/2017/e/eb/PJET_Cloning.pdf" target="_blank">pJET Cloning</a></td>
 +
    </tr>
 +
   
 +
    <tr>
 +
        <td><a href="https://static.igem.org/mediawiki/2017/0/08/Plasmid_Miniprep.pdf" target="_blank">Plasmid Miniprep</a></td>
 +
        <td><a href="https://static.igem.org/mediawiki/2017/7/78/Preparation_of_Chemical_Competent_Cells.pdf" target="_blank">Preparation of Chemical Competent Cells</a></td>
 +
    </tr>
 +
   
 +
    <tr>
 +
        <td><a href="https://static.igem.org/mediawiki/2017/2/2c/Protein_Overexpression_.pdf" target="_blank">Protein Overexpression</a></td>
 +
        <td><a href="https://static.igem.org/mediawiki/2017/5/5f/PURExpress.pdf" target="_blank">PURExpress NEB Kit</a></td>
 +
    </tr>
 +
   
 +
    <tr>
 +
        <td><a href="https://static.igem.org/mediawiki/2017/f/fc/Purification_of_Poly-Histidine_Tagged_Proteins.pdf" target="_blank">Purification of HexaHistidine Tagged Proteins</a></td>
 +
        <td><a href="https://static.igem.org/mediawiki/2017/1/10/Restriction_Enzyme_Digest_and_Dephosphorylation.pdf" target="_blank">Restriction Enzyme Digest and Dephosphorylation</a></td>
 +
    </tr>
 +
   
 +
    <tr>
 +
        <td><a href="https://static.igem.org/mediawiki/2017/2/2a/T7-RNA_Polymerase_Purification.pdf" target="_blank">T7- RNA Polymerase Purification</a></td>
 +
        <td><a href="https://static.igem.org/mediawiki/2017/a/a6/Transformation.pdf" target="_blank">Transformation</a></td>
 +
    </tr>
 +
    <tr>
 +
        <td><a href="https://static.igem.org/mediawiki/2017/c/cd/MS2-tRNA_Complex_Purification.pdf" target="_blank">MS2-tRNA Affinity Chromatography Purification</a></td>
 +
        <td></td>
 +
    </tr>
 +
</table>
 
</div>
 
</div>
 +
</center>
 
<br>
 
<br>
  
Line 87: Line 116:
 
     <td>IPTG</td>
 
     <td>IPTG</td>
 
   <td>Prefix Forward: 5’GAATTCGCGGCCGCTTCTAG3’</td>
 
   <td>Prefix Forward: 5’GAATTCGCGGCCGCTTCTAG3’</td>
−
</td>
 
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
 
     <td>Phusion Polymerase Master Mix</td>
 
     <td>Phusion Polymerase Master Mix</td>
−
     <td>Kanomycin/Chloramphenicol/Ampicillin </td>
+
     <td>Kanomycin / Chloramphenicol / Ampicillin </td>
 
   <td>Prefix Reverse: 5’CTGCAGCGGCCGCTACTAGTA3’</td>
 
   <td>Prefix Reverse: 5’CTGCAGCGGCCGCTACTAGTA3’</td>
 
   </tr>
 
   </tr>
Line 107: Line 135:
 
     <td>T7 DNA Polymerase</td>
 
     <td>T7 DNA Polymerase</td>
 
     <td>T7 RNA Polymerase Purification Buffers</td>
 
     <td>T7 RNA Polymerase Purification Buffers</td>
−
     <td>Dialyzation Membrane</td>
+
     <td>Dialysis Tubing</td>
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
Line 145: Line 173:
 
</tr>
 
</tr>
 
<tr>
 
<tr>
−
       <td>RNAse A</td>
+
       <td>RNase A</td>
 
       <td>SDS-PAGE Reagents</td>
 
       <td>SDS-PAGE Reagents</td>
 
     <td></td>
 
     <td></td>
Line 160: Line 188:
 
</tr>
 
</tr>
 
<tr>
 
<tr>
−
     <td>RNAse Inhibitor</td>
+
     <td>RNase Inhibitor</td>
 
     <td>Agar</td>
 
     <td>Agar</td>
 
     <td></td>
 
     <td></td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
−
     <td>DNAseI</td>
+
     <td>DNaseI</td>
 
     <td>Tryptone</td>
 
     <td>Tryptone</td>
 
     <td></td>
 
     <td></td>
Line 178: Line 206:
 
     <td>TAKMz</td>
 
     <td>TAKMz</td>
 
     <td> </td>
 
     <td> </td>
−
</tr
+
</tr>
 
<tr>
 
<tr>
 
     <td></td>
 
     <td></td>
Line 194: Line 222:
 
<br>
 
<br>
 
<br>
 
<br>
−
<img src="https://static.igem.org/mediawiki/2017/7/7d/Banner_footer_blank.png" class="bannerImg" />
+
</div><!-- closing #allContent -->
 +
<img src="https://static.igem.org/mediawiki/2017/7/7d/Banner_footer_blank.png" class="bannerImg">
 
</body>
 
</body>
 
</html>
 
</html>

Latest revision as of 03:07, 2 November 2017

top

Protocols


Products/Reagents

Enzymes Buffers/Reagents Other Products
Shrimp Alkaline Phosphatase Affinity Chromatography Buffers Ni²+ Affinity Resin
DNA Blunting Enzyme IPTG Prefix Forward: 5’GAATTCGCGGCCGCTTCTAG3’
Phusion Polymerase Master Mix Kanomycin / Chloramphenicol / Ampicillin Prefix Reverse: 5’CTGCAGCGGCCGCTACTAGTA3’
PFU Polymerase Dimethyl Sulfur Dioxide Prefix Forward +6:5’GGCTCAGAATTCGCGGCCGCTTCTAGAG3’
T7 RNA Polymerase Competent Cell Buffers Suffix Reverse +6: 5’CTGGACCTGCAGCGGCCGCTACTAGTA3’
T7 DNA Polymerase T7 RNA Polymerase Purification Buffers Dialysis Tubing
PURExpressKit (New England Biolabs) Phenol
XbaI (NEB) Chloroform
SpeI (NEB) 10x PFU Buffer
PstI (NEB) NTP
EcoRI (NEB) GMP
Dream Taq DNA Polymerase 10x Taq Buffer
Taq DNA Polymerase Ligase Buffer
RNase A SDS-PAGE Reagents
TraB Urea PAGE reagents
iPPase Agarose
RNase Inhibitor Agar
DNaseI Tryptone
Yeast Extract
TAKMz
BioBasic Plasmid DNA miniprep Kit
Imidazole