Difference between revisions of "Team:XJTLU-CHINA/Protocols"
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<a href="https://2017.igem.org/Team:XJTLU-CHINA/Protocols_Electroporation"><h3>Electroporation for <i>Lactococcus lactis</i></h3></a> | <a href="https://2017.igem.org/Team:XJTLU-CHINA/Protocols_Electroporation"><h3>Electroporation for <i>Lactococcus lactis</i></h3></a> | ||
<a href="https://2017.igem.org/Team:XJTLU-CHINA/Protocols_Plasmid_DNA_isolation"><h3>Plasmid DNA isolation from <i>Lactococcus lactis</i></h3></a> | <a href="https://2017.igem.org/Team:XJTLU-CHINA/Protocols_Plasmid_DNA_isolation"><h3>Plasmid DNA isolation from <i>Lactococcus lactis</i></h3></a> | ||
| + | <a href="https://2017.igem.org/Team:XJTLU-CHINA/Protocols_Dot_blot"><h3>Dot Blot</h3></a> | ||
<hr> | <hr> | ||
<h1>Anti-microbial peptides assay:</h1> | <h1>Anti-microbial peptides assay:</h1> | ||
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<a href="https://2017.igem.org/Team:XJTLU-CHINA/Protocols_Testing_sensing_pathway"><h3>Testing of <i>S. aureus</i> sensing pathway</h3></a> | <a href="https://2017.igem.org/Team:XJTLU-CHINA/Protocols_Testing_sensing_pathway"><h3>Testing of <i>S. aureus</i> sensing pathway</h3></a> | ||
<a href="https://2017.igem.org/Team:XJTLU-CHINA/Protocols_Autolysis_test"><h3>Autolysis test</h3></a> | <a href="https://2017.igem.org/Team:XJTLU-CHINA/Protocols_Autolysis_test"><h3>Autolysis test</h3></a> | ||
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</div><!-- container --!> | </div><!-- container --!> | ||
Revision as of 10:41, 26 November 2017
Protocols
Basic Molecular Biology Techniques:
PCR using Q5 Hi-Fi DNA polymerase Master Mix
PCR purification using QIAquick PCR purification kit
Gel Extraction
Gibson Assembly
Digestion (NEB enzymes)
Ligation
Transformation of plasmids in E.coli DH5ɑ
Isolation of plasmids in E.coli DH5ɑ
Preparation of competent Lactococcus lactis
Electroporation for Lactococcus lactis
Plasmid DNA isolation from Lactococcus lactis
Dot Blot
Anti-microbial peptides assay:
Pre-experiment: CFU measurement
Inhibition ring
AMPs efficiency and MIC assay
Biofilm formation test
Growth curve measurement
Nisin induction of gene expression in Lactococcus lactis:
Testing of S. aureus sensing pathway
Autolysis test
Collaborators and Supporters
