Difference between revisions of "Team:Pasteur Paris/InterLab"

(Prototype team page)
 
 
(23 intermediate revisions by 4 users not shown)
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−
{{Pasteur_Paris}}
+
<html lang="en">
−
<html>
+
  <head>
 +
    <meta charset="utf-8">
 +
    <meta name="description" content="igem">
 +
    <meta name="keywords" content="igem, biology">
 +
    <meta name="copyright" content="© Copyright 2016 - iGEM Pasteur"% />
 +
    <meta name="author" content="Pasteur">
 +
    <meta name="location" content="Paris, FRANCE">
 +
    <meta http-equiv="x-ua-compatible" content="ie=edge">
 +
    <meta name="viewport" content="width=device-width, initial-scale=1">
 +
    <link rel="icon" type="image/png" href="./static/favicon.png" />
 +
<head>
 +
<link rel="stylesheet" href="https://cdnjs.cloudflare.com/ajax/libs/font-awesome/4.7.0/css/font-awesome.min.css">
 +
<link rel="stylesheet" href="https://fonts.googleapis.com/icon?family=Material+Icons">
  
  
−
<div class="column full_size judges-will-not-evaluate">
+
<script>
−
<h3>★  ALERT! </h3>
+
 
−
<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
+
/*Fonction apparition onglet après click sur le menu*/
−
<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
+
function apparitionTeam() {
 +
    document.getElementById("Team").style.display = "block";
 +
    document.getElementById("Project").style.display = "none";
 +
    document.getElementById("Design").style.display = "none";
 +
    document.getElementById("Labwork").style.display = "none";
 +
    document.getElementById("HumanPractice").style.display = "none";
 +
    document.getElementById("Achievements").style.display = "none";
 +
}
 +
function apparitionProject() {
 +
    document.getElementById("Project").style.display = "block";
 +
    document.getElementById("Team").style.display = "none";
 +
    document.getElementById("Design").style.display = "none";
 +
    document.getElementById("Labwork").style.display = "none";
 +
    document.getElementById("HumanPractice").style.display = "none";
 +
    document.getElementById("Achievements").style.display = "none";
 +
}
 +
function apparitionDesign() {
 +
    document.getElementById("Design").style.display = "block";
 +
    document.getElementById("Team").style.display = "none";
 +
    document.getElementById("Project").style.display = "none";
 +
    document.getElementById("Labwork").style.display = "none";
 +
    document.getElementById("HumanPractice").style.display = "none";
 +
    document.getElementById("Achievements").style.display = "none";
 +
}
 +
function apparitionLabWork() {
 +
    document.getElementById("Labwork").style.display = "block";
 +
    document.getElementById("Team").style.display = "none";
 +
    document.getElementById("Project").style.display = "none";
 +
    document.getElementById("Design").style.display = "none";
 +
    document.getElementById("HumanPractice").style.display = "none";
 +
    document.getElementById("Achievements").style.display = "none";
 +
}
 +
function apparitionHumanPractice() {
 +
    document.getElementById("HumanPractice").style.display = "block";
 +
    document.getElementById("Team").style.display = "none";
 +
    document.getElementById("Project").style.display = "none";
 +
    document.getElementById("Design").style.display = "none";
 +
    document.getElementById("Labwork").style.display = "none";
 +
    document.getElementById("Achievements").style.display = "none";
 +
}
 +
function apparitionAchievements() {
 +
    document.getElementById("Achievements").style.display = "block";
 +
    document.getElementById("Team").style.display = "none";
 +
    document.getElementById("Project").style.display = "none";
 +
    document.getElementById("Design").style.display = "none";
 +
    document.getElementById("HumanPractice").style.display = "none";
 +
    document.getElementById("Labwork").style.display = "none";
 +
}
 +
/*////////////////////////////////////////////*/
 +
 
 +
/* Fonction pour apparition barre rouge onglet*/
 +
 
 +
function apparitionbaton() {
 +
    document.getElementById("batonbleu").style.display = "block";
 +
}
 +
 
 +
function deletebaton() {
 +
    document.getElementById("batonbleu").style.display = "none";
 +
}
 +
 
 +
function apparitionbaton1() {
 +
    document.getElementById("batonbleu1").style.display = "block";
 +
}
 +
 
 +
function deletebaton1() {
 +
    document.getElementById("batonbleu1").style.display = "none";
 +
}
 +
 
 +
function apparitionbaton1b() {
 +
    document.getElementById("batonbleu1b").style.display = "block";
 +
}
 +
 
 +
function deletebaton1b() {
 +
    document.getElementById("batonbleu1b").style.display = "none";
 +
}
 +
 
 +
function apparitionbaton2() {
 +
    document.getElementById("batonbleu2").style.display = "block";
 +
}
 +
 
 +
function deletebaton2() {
 +
    document.getElementById("batonbleu2").style.display = "none";
 +
}
 +
 
 +
function apparitionbaton3() {
 +
    document.getElementById("batonbleu3").style.display = "block";
 +
}
 +
 
 +
function deletebaton3() {
 +
    document.getElementById("batonbleu3").style.display = "none";
 +
}
 +
 
 +
function apparitionbaton4() {
 +
    document.getElementById("batonbleu4").style.display = "block";
 +
}
 +
 
 +
function deletebaton4() {
 +
    document.getElementById("batonbleu4").style.display = "none";
 +
}
 +
 
 +
function apparitionbaton5() {
 +
    document.getElementById("batonbleu5").style.display = "block";
 +
}
 +
 
 +
function deletebaton5() {
 +
    document.getElementById("batonbleu5").style.display = "none";
 +
}
 +
 
 +
function apparitionbaton6() {
 +
    document.getElementById("batonbleu6").style.display = "block";
 +
}
 +
 
 +
function deletebaton6() {
 +
    document.getElementById("batonbleu6").style.display = "none";
 +
}
 +
 
 +
function apparitionbaton7() {
 +
    document.getElementById("batonbleu7").style.display = "block";
 +
}
 +
 
 +
function deletebaton7() {
 +
    document.getElementById("batonbleu7").style.display = "none";
 +
}
 +
 
 +
function apparitionbaton8() {
 +
    document.getElementById("batonbleu8").style.display = "block";
 +
}
 +
 
 +
function deletebaton8() {
 +
    document.getElementById("batonbleu8").style.display = "none";
 +
}
 +
 
 +
function apparitionbaton8b() {
 +
    document.getElementById("batonbleu8b").style.display = "block";
 +
}
 +
 
 +
function deletebaton8b() {
 +
    document.getElementById("batonbleu8b").style.display = "none";
 +
}
 +
 
 +
function apparitionbaton9() {
 +
    document.getElementById("batonbleu9").style.display = "block";
 +
}
 +
 
 +
function deletebaton9() {
 +
    document.getElementById("batonbleu9").style.display = "none";
 +
}
 +
 
 +
function apparitionbaton10() {
 +
    document.getElementById("batonbleu10").style.display = "block";
 +
}
 +
 
 +
function deletebaton10() {
 +
    document.getElementById("batonbleu10").style.display = "none";
 +
}
 +
 
 +
function apparitionbaton11() {
 +
    document.getElementById("batonbleu11").style.display = "block";
 +
}
 +
 
 +
function deletebaton11() {
 +
    document.getElementById("batonbleu11").style.display = "none";
 +
}
 +
 
 +
function apparitionbaton12() {
 +
    document.getElementById("batonbleu12").style.display = "block";
 +
}
 +
 
 +
function deletebaton12() {
 +
    document.getElementById("batonbleu12").style.display = "none";
 +
}
 +
 
 +
function apparitionbaton13() {
 +
    document.getElementById("batonbleu13").style.display = "block";
 +
}
 +
 
 +
function deletebaton13() {
 +
    document.getElementById("batonbleu13").style.display = "none";
 +
}
 +
 
 +
function apparitionbaton14() {
 +
    document.getElementById("batonbleu14").style.display = "block";
 +
}
 +
 
 +
function deletebaton14() {
 +
    document.getElementById("batonbleu14").style.display = "none";
 +
}
 +
 
 +
function apparitionbaton15() {
 +
    document.getElementById("batonbleu15").style.display = "block";
 +
}
 +
 
 +
function deletebaton15() {
 +
    document.getElementById("batonbleu15").style.display = "none";
 +
}
 +
 
 +
function apparitionbaton16() {
 +
    document.getElementById("batonbleu16").style.display = "block";
 +
}
 +
 
 +
function deletebaton16() {
 +
    document.getElementById("batonbleu16").style.display = "none";
 +
}
 +
 
 +
/*////////////////////////////////////////////*/
 +
 
 +
function CarnotNoiretBlanc() {
 +
  document.getElementById("CarnotBW").style.display = "block";
 +
  document.getElementById("Carnot").style.display = "none";
 +
}
 +
 
 +
function CarnotCouleur() {
 +
  document.getElementById("Carnot").style.display = "block";
 +
  document.getElementById("CarnotBW").style.display = "none";
 +
}
 +
 
 +
 
 +
function GlobalCareNoiretBlanc() {
 +
  document.getElementById("GlobalCareBW").style.display = "block";
 +
  document.getElementById("GlobalCare").style.display = "none";
 +
}
 +
 
 +
function GlobalCareCouleur() {
 +
  document.getElementById("GlobalCare").style.display = "block";
 +
  document.getElementById("GlobalCareBW").style.display = "none";
 +
}
 +
 
 +
function MettlerToledoNoiretBlanc() {
 +
  document.getElementById("MettlerToledoBW").style.display = "block";
 +
  document.getElementById("MettlerToledo").style.display = "none";
 +
}
 +
 
 +
function MettlerToledoCouleur() {
 +
  document.getElementById("MettlerToledo").style.display = "block";
 +
  document.getElementById("MettlerToledoBW").style.display = "none";
 +
}
 +
 
 +
function geniousNoiretBlanc() {
 +
  document.getElementById("geniousBW").style.display = "block";
 +
  document.getElementById("genious").style.display = "none";
 +
}
 +
 
 +
function geniousCouleur() {
 +
  document.getElementById("genious").style.display = "block";
 +
  document.getElementById("geniousBW").style.display = "none";
 +
}
 +
 
 +
function centraleNoiretBlanc() {
 +
  document.getElementById("CentraleBW").style.display = "block";
 +
  document.getElementById("Centrale").style.display = "none";
 +
}
 +
 
 +
function centraleCouleur() {
 +
  document.getElementById("Centrale").style.display = "block";
 +
  document.getElementById("CentraleBW").style.display = "none";
 +
}
 +
 
 +
function ambassadeNoiretBlanc() {
 +
  document.getElementById("ambassadeBW").style.display = "block";
 +
  document.getElementById("ambassade").style.display = "none";
 +
}
 +
 
 +
function ambassadeCouleur() {
 +
  document.getElementById("ambassade").style.display = "block";
 +
  document.getElementById("ambassadeBW").style.display = "none";
 +
}
 +
 
 +
function QuiagenNoiretBlanc() {
 +
  document.getElementById("QuiagenBW").style.display = "block";
 +
  document.getElementById("Quiagen").style.display = "none";
 +
}
 +
 
 +
function QuiagenCouleur() {
 +
  document.getElementById("Quiagen").style.display = "block";
 +
  document.getElementById("QuiagenBW").style.display = "none";
 +
}
 +
 
 +
function eurofinsNoiretBlanc() {
 +
  document.getElementById("eurofinsBW").style.display = "block";
 +
  document.getElementById("eurofins").style.display = "none";
 +
}
 +
 
 +
function eurofinsCouleur() {
 +
  document.getElementById("eurofins").style.display = "block";
 +
  document.getElementById("eurofinsBW").style.display = "none";
 +
}
 +
 
 +
function ThermofisherNoiretBlanc() {
 +
  document.getElementById("ThermofisherBW").style.display = "block";
 +
  document.getElementById("Thermofisher").style.display = "none";
 +
}
 +
 
 +
function ThermofisherCouleur() {
 +
  document.getElementById("Thermofisher").style.display = "block";
 +
  document.getElementById("ThermofisherBW").style.display = "none";
 +
}
 +
 
 +
function BarthenteimNoiretBlanc() {
 +
  document.getElementById("BarthenteimBW").style.display = "block";
 +
  document.getElementById("Barthenteim").style.display = "none";
 +
}
 +
 
 +
function BarthenteimCouleur() {
 +
  document.getElementById("Barthenteim").style.display = "block";
 +
  document.getElementById("BarthenteimBW").style.display = "none";
 +
}
 +
 
 +
</script>
 +
<style>
 +
 
 +
@font-face {
 +
  font-family: "CrimsonTextSemi";
 +
  src: url("https://static.igem.org/mediawiki/2017/7/75/T--Pasteur_Paris--CrimsonText-Semibold.ttf");
 +
}
 +
 
 +
@font-face {
 +
  font-family: "CrimsonTextRoman";
 +
  src: url("https://static.igem.org/mediawiki/2017/0/0e/T--Pasteur_Paris--CrimsonText-Roman.ttf");
 +
}
 +
 
 +
body{
 +
  margin:30px;
 +
  padding:30px;
 +
  background-color:white;
 +
  height:100%;
 +
}
 +
 
 +
a{
 +
  text-decoration: none;
 +
  color:black;
 +
}
 +
 
 +
li{
 +
  list-style:none;
 +
}
 +
 
 +
.left{
 +
  left: 30px;
 +
  margin-bottom: 7.5px;
 +
  padding-top: 37.5px;
 +
  position:fixed;
 +
  background-color: none;
 +
  top:0;
 +
}
 +
 
 +
.right{
 +
  right: 30px;
 +
  margin-bottom: 7.5px; 
 +
  padding-top: 37.5px;
 +
  position:fixed;
 +
  background-color: none;
 +
  top:0;
 +
}
 +
 
 +
.center{
 +
    margin-left: auto;
 +
    margin-right: auto;
 +
}
 +
 
 +
/*Position menu*/
 +
.menu { 
 +
    text-align: left;
 +
    width: 450px;
 +
    position: fixed;
 +
    left:30px;
 +
    line-height: 200%;
 +
    font-family: Helvetica, sans-serif;
 +
    font-weight: bold;
 +
    font-size: 7.5pt;
 +
    display: flex;
 +
    flex-direction: column-reverse;
 +
    text-align: bottom left;
 +
    bottom: 4%;
 +
}
 +
 
 +
.menu span{
 +
  cursor: pointer;
 +
}
 +
 
 +
.com{
 +
    display: flex;
 +
    flex-direction: column;
 +
    position:fixed;
 +
    text-align: left;
 +
    right:30px;
 +
    bottom: 4%;
 +
    color:black;
 +
}
 +
 
 +
/*Ensemble pour trait après le menu*/
 +
 
 +
.lastword {
 +
    width: 150px;
 +
}
 +
 
 +
.lastword img {
 +
    position:absolute;
 +
    opacity:0;
 +
}
 +
 
 +
.lastword:hover:after{
 +
    display:inline-block;
 +
    vertical-align: middle;
 +
    height:3px;
 +
    width:22.5px;
 +
    background: #3298CB;
 +
    content:""
 +
}
 +
 
 +
/*////////////////////////////////////////////*/
 +
 
 +
.com a:hover{
 +
  color: #3298CB;
 +
}
 +
 
 +
.com li{
 +
  line-height:200%;
 +
  vertical-align: middle;
 +
}
 +
 
 +
.underline{
 +
  display:block;
 +
  position:relative;
 +
  top:-15px;
 +
  left:0px;
 +
  border-bottom:4px dotted black;
 +
  width: 295px;
 +
}
 +
 
 +
.underline1{
 +
  display:block;
 +
  position:relative;
 +
  top:-15px;
 +
  left:0px;
 +
  border-bottom:4px dotted black;
 +
  width: 220px;
 +
}
 +
 
 +
.underline2{
 +
  display:block;
 +
  position:relative;
 +
  top:-15px;
 +
  left:0px;
 +
  border-bottom:4px dotted black;
 +
  width: 72px;
 +
}
 +
 
 +
.underline3{
 +
  display:block;
 +
  position:relative;
 +
  top:-15px;
 +
  left:0px;
 +
  border-bottom:4px dotted black;
 +
  width: 192px;
 +
}
 +
 
 +
#top_title{
 +
display:none;
 +
}
 +
 
 +
#sideMenu{
 +
display:none;
 +
position:fixed;
 +
right:120px;
 +
}
 +
 
 +
#content{
 +
  width:100%;
 +
  margin: 0px 0px 0px 0px;
 +
  padding: 0px 0px 0px 0px;
 +
}
 +
 
 +
.title{
 +
  font-size: 48.75pt;
 +
  margin-left:300px;
 +
  margin-top:112.5px;
 +
  cursor: default;
 +
}
 +
 
 +
.subtitle{
 +
  margin-left:12.25px;
 +
  margin-top:55px;
 +
  cursor: default;
 +
  font-size: 18pt;
 +
}
 +
 
 +
 
 +
.maintext{
 +
    font-size: 9pt;
 +
    font-family:Helvetica, sans-serif;
 +
    margin-top:25px;
 +
    text-align: justify;
 +
    cursor: default;
 +
    width:65%;
 +
    line-height:12pt;
 +
}
 +
 
 +
#subtitles{
 +
  position:relative;
 +
  margin-left: 287.75px;
 +
  top:10px;
 +
  font-size: 18pt;
 +
  font-weight: normal;
 +
  /*border-bottom:4px dotted black;*/
 +
  z-index:-1;
 +
  width: 570px;
 +
  border-bottom:4px dotted black;
 +
}
 +
 
 +
.maintextsuite{
 +
    font-size: 9pt;
 +
    margin-top:10px;
 +
    text-align: justify;
 +
    cursor: default;
 +
    font-family:Helvetica, sans-serif;
 +
    width:65%;
 +
    line-height:12pt;
 +
}
 +
 
 +
#title{
 +
  font-size: 60pt;
 +
  border-bottom:4px dotted black;
 +
  width: 205px;
 +
  z-index:-1;
 +
  margin-left: 287.75px;
 +
  margin-top: 90px;
 +
}
 +
 
 +
.maintextsuiteimage{
 +
    font-size: 9pt;
 +
    margin-top:10px;
 +
    text-align: justify;
 +
    cursor: default;
 +
    font-family:Helvetica, sans-serif;
 +
    width:40%;
 +
    line-height:12pt;
 +
}
 +
 
 +
.text {
 +
  position:relative;
 +
  margin-left: 287.75px;
 +
}
 +
 
 +
#logo{
 +
  font-size:18px;
 +
text-decoration:none;
 +
color:black;
 +
}
 +
 
 +
#logo:hover{
 +
  color: #3298CB;
 +
}
 +
 
 +
/*Flèche de remontée*/
 +
 
 +
#toparrow{
 +
    justify-content: center;
 +
    position:fixed;
 +
    vertical-align:middle;
 +
    z-index:10;
 +
    width:2%;
 +
    top:42%;
 +
    right:30px;
 +
}
 +
 
 +
#toparrow a:hover{
 +
  color: #3298CB;
 +
}
 +
 
 +
.Chili{
 +
  z-index:-1;
 +
  margin-left: 287.75px;
 +
  top:90px;
 +
  width:56vw;
 +
  height: 55vh;
 +
}
 +
 
 +
table{
 +
  border-collapse: collapse;
 +
  }
 +
 
 +
.outer {
 +
  position: fixed;
 +
  left: 40px;
 +
  top: 28%;
 +
}
 +
 
 +
.outer1 {
 +
  position: fixed;
 +
  left: 40px;
 +
  top: 38%;
 +
}
 +
 
 +
.outer1b {
 +
  position: fixed;
 +
  left: 40px;
 +
  top: 47.5%;
 +
}
 +
 
 +
.outer2 {
 +
  position: fixed;
 +
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 +
/*////////////////////////////////////////////*/
 +
 
 +
 
 +
.rotate {
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  -webkit-transform: rotate(180deg);
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 +
 
 +
#Team, #Design, #Project, #HumanPractice, #Achievements, #Labwork{
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 +
    font-weight: bold;
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    font-size: 8pt;
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 +
/*Trait rouge des onglets*/
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 +
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 +
 
 +
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 +
    position:relative;
 +
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 +
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 +
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 +
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 +
    width:22.5px;
 +
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 +
 
 +
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 +
    position:relative;
 +
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 +
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 +
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 +
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 +
    width:22.5px;
 +
    background: #3298CB;
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 +
 
 +
#batonbleu16{
 +
    position:relative;
 +
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 +
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 +
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 +
}
 +
 
 +
li{margin:5px}
 +
 
 +
/*////////////////////////////////////////////*/
 +
 
 +
</style>
 +
  </head>
 +
 
 +
 
 +
  <body>
 +
  <a class="left" href="https://2017.igem.org/Team:Pasteur_Paris"><img src="https://static.igem.org/mediawiki/2017/d/d8/T--Pasteur_Paris--aether.png"; width="220px"; height="70px"></img></a>
 +
  <a class="right" href="https://2017.igem.org/Team:Pasteur_Paris"><img src="https://static.igem.org/mediawiki/2017/1/10/T--Pasteur_Paris--iGEM.png"; width="90px"; height="70px"></img></a>
 +
<nav class="menu">
 +
  <ul>
 +
    <li><FONT size ="2.25pt" color=#00000><span class="lastword"; onclick="apparitionTeam()">Team </span></FONT></li>
 +
    <li><FONT size ="2.25pt" color=#00000><span class="lastword"; onclick="apparitionProject()">Project </span></FONT></li>
 +
    <li><FONT size ="2.25pt" color=#00000><span class="lastword"; onclick="apparitionDesign()">Design </span></FONT></li>
 +
    <li><FONT size ="2.25pt" color=#00000><span class="lastword"; onclick="apparitionLabWork()">Lab work </span></FONT></li>
 +
    <li><FONT size ="2.25pt" color=#00000><span class="lastword"; onclick="apparitionHumanPractice()">Human Practice </span></FONT></li>
 +
    <li><FONT size ="2.25pt" color=#00000><span class="lastword"; onclick="apparitionAchievements()">Achievements </span></FONT></li>
 +
  </ul>
 +
</nav>
 +
<div id="Team" style="display:none">
 +
  <div class="outer">
 +
    <div class="inner"; onmouseover="apparitionbaton()"; onmouseout="deletebaton()">
 +
      <div class="list rotate">
 +
        <a href="https://2017.igem.org/Team:Pasteur_Paris/Team">Our Team </a>
 +
        <div id="batonbleu">
 +
        </div>
 +
      </div>
 +
    </div>
 +
  </div>
 +
  <div class="outer1">
 +
    <div class="inner"; onmouseover="apparitionbaton1()"; onmouseout="deletebaton1()">
 +
      <div class="list rotate">
 +
        <a href="https://2017.igem.org/Team:Pasteur_Paris/Team#pagesponsors">Sponsors </a>
 +
        <div id="batonbleu1">
 +
        </div>
 +
      </div>
 +
    </div>
 +
  </div>
 +
  <div class="outer1b">
 +
    <div class="inner"; onmouseover="apparitionbaton1b()"; onmouseout="deletebaton1b()">
 +
      <div class="list rotate">
 +
        <a href="https://2017.igem.org/Team:Pasteur_Paris/Attributions">Attributions </a>
 +
        <div id="batonbleu1b">
 +
        </div>
 +
      </div>
 +
    </div>
 +
  </div>
 
</div>
 
</div>
−
<div class="clear"></div>
 
  
−
<div class="column full_size">
+
<div id="Project" style="display:none">
−
<h1>InterLab</h1>
+
  <div class="outer2">
−
<h3>Bronze Medal Criterion #4</h3>
+
    <div class="inner"; onmouseover="apparitionbaton2()"; onmouseout="deletebaton2()">
−
<p><b>Standard Tracks:</b> Participate in the Interlab Measurement Study and/or improve the characterization of an existing BioBrick Part or Device and enter this information on that part's Main Page in the Registry. The part that you are characterizing must NOT be from a 2017 part number range.
+
      <div class="list rotate">
−
<br><br>
+
        <a href="https://2017.igem.org/Team:Pasteur_Paris/Project">Context</a>
−
For teams participating in the <a href="https://2017.igem.org/Competition/InterLab_Study">InterLab study</a>, all work must be shown on this page.
+
        <div id="batonbleu2">
 +
        </div>
 +
      </div>
 +
    </div>
 +
  </div>
 +
  <div class="outer3">
 +
    <div class="inner"; onmouseover="apparitionbaton3()"; onmouseout="deletebaton3()">
 +
      <div class="list rotate">
 +
        <a href="https://2017.igem.org/Team:Pasteur_Paris/Entrepreneurship">Entrepreneurship</a>
 +
        <div id="batonbleu3">
 +
        </div>
 +
      </div>
 +
  </div>
 +
  </div>
 +
    <div class="outer3a">
 +
    <div class="inner"; onmouseover="apparitionbaton3a()"; onmouseout="deletebaton3a()">
 +
      <div class="list rotate">
 +
        <a href="https://2017.igem.org/Team:Pasteur_Paris/Engagement">Engagement</a>
 +
        <div id="batonbleu3a">
 +
        </div>
 +
      </div>
 +
    </div>
 +
  </div>
 +
  <div class="outer3b">
 +
    <div class="inner"; onmouseover="apparitionbaton3b()"; onmouseout="deletebaton3b()">
 +
      <div class="list rotate">
 +
        <a href="https://2017.igem.org/Team:Pasteur_Paris/Model">Model</a>
 +
        <div id="batonbleu3b">
 +
        </div>
 +
      </div>
 +
    </div>
 +
  </div>
 +
</div>
  
−
</p>
+
<div id="Design" style="display:none">
 +
  <div class="outer4">
 +
    <div class="inner"; onmouseover="apparitionbaton4()"; onmouseout="deletebaton4()">
 +
      <div class="list rotate">
 +
        <a href="https://2017.igem.org/Team:Pasteur_Paris/Design">Science</a>
 +
        <div id="batonbleu4">
 +
        </div>
 +
      </div>
 +
    </div>
 +
  </div>
 +
  <div class="outer5">
 +
    <div class="inner"; onmouseover="apparitionbaton5()"; onmouseout="deletebaton5()">
 +
      <div class="list rotate">
 +
        <a href="https://2017.igem.org/Team:Pasteur_Paris/Design">Product</a>
 +
        <div id="batonbleu5">
 +
        </div>
 +
      </div>
 +
    </div>
 +
  </div>
 +
  <div class="outer6">
 +
    <div class="inner"; onmouseover="apparitionbaton6()"; onmouseout="deletebaton6()">
 +
      <div class="list rotate">
 +
        <a href="https://2017.igem.org/Team:Pasteur_Paris/Design">Scenario</a>
 +
        <div id="batonbleu6">
 +
        </div>
 +
      </div>
 +
    </div>
 +
  </div>
 +
  <div class="outer7">
 +
    <div class="inner"; onmouseover="apparitionbaton7()"; onmouseout="deletebaton7()">
 +
      <div class="list rotate">
 +
        <a href="https://2017.igem.org/Team:Pasteur_Paris/Applied_Design">Applied<br>Design</a>
 +
        <div id="batonbleu7">
 +
        </div>
 +
      </div>
 +
    </div>
 +
  </div>
 
</div>
 
</div>
  
 +
<div id="Labwork" style="display:none">
 +
  <div class="outer8">
 +
    <div class="inner"; onmouseover="apparitionbaton8()"; onmouseout="deletebaton8()">
 +
      <div class="list rotate">
 +
        <a href="https://2017.igem.org/Team:Pasteur_Paris/Notebook">Notebook</a>
 +
        <div id="batonbleu8">
 +
        </div>
 +
      </div>
 +
    </div>
 +
  </div>
 +
  <div class="outer8b">
 +
    <div class="inner"; onmouseover="apparitionbaton8b()"; onmouseout="deletebaton8b()">
 +
      <div class="list rotate">
 +
        <a href="https://2017.igem.org/Team:Pasteur_Paris/Demonstrate">Demonstrate</a>
 +
        <div id="batonbleu8b">
 +
        </div>
 +
      </div>
 +
    </div>
 +
  </div>
 +
  <div class="outer9">
 +
    <div class="inner"; onmouseover="apparitionbaton9()"; onmouseout="deletebaton9()">
 +
      <div class="list rotate">
 +
        <a href="https://2017.igem.org/Team:Pasteur_Paris/InterLab">InterLab</a>
 +
        <div id="batonbleu9">
 +
        </div>
 +
      </div>
 +
    </div>
 +
  </div>
 +
  <div class="outer10">
 +
    <div class="inner"; onmouseover="apparitionbaton10()"; onmouseout="deletebaton10()">
 +
      <div class="list rotate">
 +
        <a href="https://2017.igem.org/Team:Pasteur_Paris/Protocol">Protocol</a>
 +
        <div id="batonbleu10">
 +
        </div>
 +
      </div>
 +
    </div>
 +
  </div>
 +
  <div class="outer11">
 +
    <div class="inner"; onmouseover="apparitionbaton11()"; onmouseout="deletebaton11()">
 +
      <div class="list rotate">
 +
        <a href="https://2017.igem.org/Team:Pasteur_Paris/Safety">Safety<br>Security</a>
 +
        <div id="batonbleu11">
 +
        </div>
 +
      </div>
 +
    </div>
 +
  </div>
 +
</div>
  
 +
<div id="HumanPractice" style="display:none">
 +
  <div class="outer12">
 +
    <div class="inner"; onmouseover="apparitionbaton12()"; onmouseout="deletebaton12()">
 +
      <div class="list rotate">
 +
      <a href="https://2017.igem.org/Team:Pasteur_Paris/HP/Law">Law</a>
 +
      <div id="batonbleu12"></div>
 +
      </div>
 +
    </div>
 +
  </div>
 +
  <div class="outer13">
 +
    <div class="inner"; onmouseover="apparitionbaton13()"; onmouseout="deletebaton13()">
 +
      <div class="list rotate">
 +
        <a href="https://2017.igem.org/Team:Pasteur_Paris/Collaborations">Collaborations</a>
 +
        <div id="batonbleu13">
 +
        </div>
 +
      </div>
 +
    </div>
 +
  </div>
 +
  <div class="outer13b">
 +
    <div class="inner"; onmouseover="apparitionbaton13b()"; onmouseout="deletebaton13b()">
 +
      <div class="list rotate">
 +
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        <div id="batonbleu14">
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        </div>
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    </div>
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    <div class="inner"; onmouseover="apparitionbaton16()"; onmouseout="deletebaton16()">
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      <div class="list rotate">
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        <div id="batonbleu16">
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        </div>
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      </div>
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</div>
  
  
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<div style="height:75%">
 +
  <img src="https://static.igem.org/mediawiki/2017/2/2d/HQ_interlab_2016.jpg"; style="position:absolute; left:390px; height: 66.5vh;width:auto;max-width: 60vw; z-index:-2;top:-70px"/>
 +
  <p id="title" style="font-size:50pt; font-family: CrimsonTextRoman, serif;">Interlab</p>
 +
  <div class="Chili"></div>
 +
  <p id="subtitles" style=" font-family: CrimsonTextRoman, serif;font-size: 18pt">A Brief History of the InterLab (from iGEM official website)</p>
 +
</div>
 +
 +
 +
<div class="text">
 +
<p class="maintext"> Over the past three years, iGEM has advanced the frontiers of science with the two biggest interlaboratory studies ever done in synthetic biology. These studies established a baseline for replicability of fluorescence measurements and identified likely key sources of error, and have now been published as an open-access journal article in PLOS ONE. </p>
 +
 +
<div class="subtitle">
 +
  <p style="font-family: CrimsonTextRoman, serif;font-size:18pt">The 2017 iGEM Interlab Study</p>
 +
  <div class="underline"></div>
 +
</div>
 +
 +
<p class="maintext"> The aim of this year’s Interlab Study is to establish a GFP measurement protocol based on engineering principles that anyone with a plate reader can use in their lab. Teams were once more asked to use the same exact protocol around the world to produce common, comparable units for measuring GFP with different plate readers. Teams who had access to flow cytometry systems were also encouraged to make flow cytometry measurements, namely counting cells.</p>
 +
 +
<div class="subtitle">
 +
  <p style="font-family: CrimsonTextRoman, serif;font-size:18pt">Materials and methods</p>
 +
  <div class="underline1"></div>
 +
</div>
 +
 +
<p class="maintext"> The 8 plasmids (Positive Control, Negative Control, Test Device 1, Test Device 2, Test Device 3, Test Device 4, Test Device 5, and Test Device 6 - locations listed below) from Kit Plate 6 were transformed into <i>E. coli</i> DH5-alpha cells. We used this <a href="https://static.igem.org/mediawiki/2017/4/41/T--Pasteur_Paris--InterlabProtocol_pdf.pdf" target="_blank" style="text-decoration:underline">transformation protocol</a></p>
 +
 +
<p class="maintextsuite"> The machines we used are given below. Using these machines was a great opportunity to discover laboratories and people at the Institut Pasteur, and to learn new biological methods.</p>
 +
 +
<p class="maintextsuite" style="margin-left:13px"> -Incubator: Unitron Infors AGCH-4103 Bottmingen</p>
 +
 +
<p class="maintextsuite" style="margin-left:13px"> -Plate reader: VaroSkan Lux Thermo Scientific</p>
 +
 +
<p class="maintextsuite" style="margin-left:13px"> -Flow cytometer : Cytoflex S Beckman Coulter</p>
 +
 +
 +
<div class="subtitle">
 +
  <p style="font-family: CrimsonTextRoman, serif;font-size:18pt">Results</p>
 +
  <div class="underline2"></div>
 +
</div>
 +
 +
<p class="maintext"> We conducted both the experiments with the plate reader and the flow cytometer. The results can be downloaded with the links below:</p>
 +
 +
<p class="maintextsuite" style="margin-left:13px"><a href="https://static.igem.org/mediawiki/2017/b/b2/T--Pasteur_Paris--InterlabMeasurements2.xls" target="_blank" style="text-decoration:underline"> -Plate reader</a></p>
 +
 +
<p class="maintextsuite" style="margin-left:13px"><a href="https://static.igem.org/mediawiki/2017/f/f7/T--Pasteur_Paris--FlowCytometrie.xls" target="_blank" style="text-decoration:underline"> -Flow cytometry</a></p>
 +
 +
<div class="subtitle">
 +
  <p style="font-family: CrimsonTextRoman, serif;font-size:18pt">Acknowledgements</p>
 +
  <div class="underline3"></div>
 +
</div>
 +
 +
<p class="maintext">The members of our team who conducted the Interlab Study experiments were not at all familiar with the equipment used. Thus, nothing would have been possible without the precious help of a few researchers at the Pasteur Institut. We would like to thank warmly: </p>
 +
 +
<p class="maintextsuite" style="margin-left:13px">- Dr Sandrine Schmutz for giving us access to the flow cytometer and teaching us how to use it.</p>
 +
 +
<p class="maintextsuite" style="margin-left:13px">- Pierre-Henri Commere platform engineer who also taught us how to use and work on the flow cytometer</p>
 +
 +
<p class="maintextsuite" style="margin-left:13px">- Finally Maxime Chazal for explaining to us how to use the plate reader</p>
 +
 +
 +
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Latest revision as of 21:35, 15 December 2017

Interlab

A Brief History of the InterLab (from iGEM official website)

Over the past three years, iGEM has advanced the frontiers of science with the two biggest interlaboratory studies ever done in synthetic biology. These studies established a baseline for replicability of fluorescence measurements and identified likely key sources of error, and have now been published as an open-access journal article in PLOS ONE. 

The 2017 iGEM Interlab Study

The aim of this year’s Interlab Study is to establish a GFP measurement protocol based on engineering principles that anyone with a plate reader can use in their lab. Teams were once more asked to use the same exact protocol around the world to produce common, comparable units for measuring GFP with different plate readers. Teams who had access to flow cytometry systems were also encouraged to make flow cytometry measurements, namely counting cells.

Materials and methods

The 8 plasmids (Positive Control, Negative Control, Test Device 1, Test Device 2, Test Device 3, Test Device 4, Test Device 5, and Test Device 6 - locations listed below) from Kit Plate 6 were transformed into E. coli DH5-alpha cells. We used this transformation protocol

The machines we used are given below. Using these machines was a great opportunity to discover laboratories and people at the Institut Pasteur, and to learn new biological methods.

-Incubator: Unitron Infors AGCH-4103 Bottmingen

-Plate reader: VaroSkan Lux Thermo Scientific

-Flow cytometer : Cytoflex S Beckman Coulter

Results

We conducted both the experiments with the plate reader and the flow cytometer. The results can be downloaded with the links below:

-Plate reader

-Flow cytometry

Acknowledgements

The members of our team who conducted the Interlab Study experiments were not at all familiar with the equipment used. Thus, nothing would have been possible without the precious help of a few researchers at the Pasteur Institut. We would like to thank warmly:

- Dr Sandrine Schmutz for giving us access to the flow cytometer and teaching us how to use it.

- Pierre-Henri Commere platform engineer who also taught us how to use and work on the flow cytometer

- Finally Maxime Chazal for explaining to us how to use the plate reader