Difference between revisions of "Team:Austin UTexas/Parts"

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<h2>Registry Parts</h2>
 
<h2>Registry Parts</h2>
 
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<p> We submitted three parts to the registry. These parts are described below.  The link to each page is shown in bold text.</p>
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<p> We submitted thwo PhytoBrick parts to the registry. Each part is described below.  The link to each page is provided in bold text.</p>
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<h3>pH Sensors</h3>
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<h4>CpxA-CpxR</h4>
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<p>CpxA-CpxR is a two-component mechanism that is activated at pH 7.4 and repressed at pH 6.0. CpxA is an intermembrane protein that autophosphorylates at a certain external pH, CpxR (a kinase) then gets phosphorylated by CpxA and acts as a transcription factor. This system originally is a transcription factor for the virF gene, but virF was replaced with a reporter. The original sequence was found in <i>Shigella sonnei</i>, but E. coli has a homolog of these proteins so all that is required on the construct is the appropriate prefix/suffix and CpxR binding site. <b>The BioBrick can be found on the iGEM registry as: <a href="http://parts.igem.org/Part:BBa_K2097000">K2097000</a></b>, as well as on our results page <a href="https://2016.igem.org/Team:Austin_UTexas/Results#section6">here</a>.</p>
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Revision as of 18:41, 22 October 2017

Registry Parts


We submitted thwo PhytoBrick parts to the registry. Each part is described below. The link to each page is provided in bold text.

Composite Parts


Blue Chromoprotein

This is a composite part made up of BBa_K592009 (blue chromoprotein) and BBa_K608002 (a strong RBS and promoter). The protein exhibits a strong blue/purple color when expressed. BBa_K608002 is made up of a promoter, BBa_J23104, and an RBS, BBa_B0034. The BioBrick can be found on the iGEM registry as: K2097001, as well as on our results page here.


Yellow-Green Chromoprotein

This is a composite part made up of BBa_K1033916 (amajLime, a yellow-green chromoprotein) and BBa_K608006 (a medium promoter and RBS). It is made up of a medium promoter, BBa_J23110, and a medium RBS, BBa_B0032. The BioBrick can be found on the iGEM registry as: K2097002, as well as on our results page here

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