Difference between revisions of "Team:Lethbridge/Experiments"

Enzymes	Buffers/Reagents	Other Products
Shrimp Alkaline Phosphatase	Affinity Chromatography Buffers	Ni²+ Affinity Resin
DNA Blunting Enzyme	IPTG	Prefix Forward: 5’GAATTCGCGGCCGCTTCTAG3’
Phusion Polymerase Master Mix	Kanomycin/Chloramphenicol/Ampicillin	Prefix Reverse: 5’CTGCAGCGGCCGCTACTAGTA3’
PFU Polymerase	Dimethyl Sulfur Dioxide	Prefix Forward +6:5’GGCTCAGAATTCGCGGCCGCTTCTAGAG3’
T7 RNA Polymerase	Competent Cell Buffers	Suffix Reverse +6: 5’CTGGACCTGCAGCGGCCGCTACTAGTA3’
T7 DNA Polymerase	T7 RNA Polymerase Purification Buffers	Dialyzation Membrane
PURExpressKit (New England Biolabs)	Phenol
XbaI (NEB)	Chloroform
SpeI (NEB)	10x PFU Buffer
PstI (NEB)	NTP
EcoRI (NEB)	GMP
Dream Taq DNA Polymerase	10x Taq Buffer
Taq DNA Polymerase	Ligase Buffer
RNAse A	SDS-PAGE Reagents
TraB	Urea PAGE reagents
iPPase	Agarose
RNAse Inhibitor	Agar
DNAseI	Tryptone
	Yeast Extract
	TAKMz
	BioBasic Plasmid DNA miniprep Kit
	Imidazole

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 <h2 class="segmentHeader">Protocols</h2>
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 <p><a href="https://static.igem.org/mediawiki/2017/b/ba/LB_Media.pdf">LB Media</a></p>
 <p><a href="https://static.igem.org/mediawiki/2017/5/5f/Agarose_Gel_Extraction.pdf">Agarose Gel Extraction</a></p>
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 <p><a href="https://static.igem.org/mediawiki/2017/2/2a/T7-RNA_Polymerase_Purification.pdf">T7- RNA Polymerase Purification</a></p>
 <p><a href="https://static.igem.org/mediawiki/2017/a/a6/Transformation.pdf">Transformation</a></p>
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+</div>
 <br><br><br><br>