Keithaiken (Talk | contribs) |
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a:active {color: #f5af3e; background-color: transparent; text-decoration: underline;} | a:active {color: #f5af3e; background-color: transparent; text-decoration: underline;} | ||
+ | /* PRODUCT REAGENT TABLE BEGINS */ | ||
#proreaTable td, th {border: 1px solid black; text-align: center; padding: 8px;} | #proreaTable td, th {border: 1px solid black; text-align: center; padding: 8px;} | ||
#proreaTable tr:nth-child(even) {background-color: white;} | #proreaTable tr:nth-child(even) {background-color: white;} | ||
+ | /* PRODUCT REAGENT TABLE ENDS */ | ||
</style> | </style> | ||
</head> | </head> | ||
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<div id="protocolList"> | <div id="protocolList"> | ||
− | <p class="pageText"><a href="https://static.igem.org/mediawiki/2017/b/ba/LB_Media.pdf">LB Media</a></p> | + | <p class="pageText"><a href="https://static.igem.org/mediawiki/2017/b/ba/LB_Media.pdf" target="_blank">LB Media</a></p> |
− | <p class="pageText"><a href="https://static.igem.org/mediawiki/2017/5/5f/Agarose_Gel_Extraction.pdf">Agarose Gel Extraction</a></p> | + | <p class="pageText"><a href="https://static.igem.org/mediawiki/2017/5/5f/Agarose_Gel_Extraction.pdf" target="_blank">Agarose Gel Extraction</a></p> |
− | <p class="pageText"><a href="https://static.igem.org/mediawiki/2017/6/68/PCR_Amplification.pdf">PCR Amplification</a></p> | + | <p class="pageText"><a href="https://static.igem.org/mediawiki/2017/6/68/PCR_Amplification.pdf" target="_blank">PCR Amplification</a></p> |
− | <p class="pageText"><a href="https://static.igem.org/mediawiki/2017/6/66/Colony_PCR.pdf">Colony PCR</a></p> | + | <p class="pageText"><a href="https://static.igem.org/mediawiki/2017/6/66/Colony_PCR.pdf" target="_blank">Colony PCR</a></p> |
− | <p class="pageText"><a href="https://static.igem.org/mediawiki/2017/6/6f/DNA_Purification_from_Enzymatic_Reactions.pdf">DNA PUrification from Enzymatic Reactions</a></p> | + | <p class="pageText"><a href="https://static.igem.org/mediawiki/2017/6/6f/DNA_Purification_from_Enzymatic_Reactions.pdf" target="_blank">DNA PUrification from Enzymatic Reactions</a></p> |
− | <p class="pageText"><a href="https://static.igem.org/mediawiki/2017/e/e5/PCR_Product_Purification.pdf">PCR product Purification</a></p> | + | <p class="pageText"><a href="https://static.igem.org/mediawiki/2017/e/e5/PCR_Product_Purification.pdf" target="_blank">PCR product Purification</a></p> |
− | <p class="pageText"><a href="https://static.igem.org/mediawiki/2017/5/51/In_vitro_transcription.pdf">In Vitro Transcription</a></p> | + | <p class="pageText"><a href="https://static.igem.org/mediawiki/2017/5/51/In_vitro_transcription.pdf" target="_blank">In Vitro Transcription</a></p> |
− | <p class="pageText"><a href="https://static.igem.org/mediawiki/2017/6/6c/Ligation_protocol.pdf">Ligations</a></p> | + | <p class="pageText"><a href="https://static.igem.org/mediawiki/2017/6/6c/Ligation_protocol.pdf" target="_blank">Ligations</a></p> |
− | <p class="pageText"><a href="https://static.igem.org/mediawiki/2017/d/df/PhenolChloroform_extraction_%26_ethanol_percipitation.pdf">Phenol/Chloroform Extraction and Ethanol Precipitation</a></p> | + | <p class="pageText"><a href="https://static.igem.org/mediawiki/2017/d/df/PhenolChloroform_extraction_%26_ethanol_percipitation.pdf" target="_blank">Phenol/Chloroform Extraction and Ethanol Precipitation</a></p> |
<p class="pageText"><a href="https://static.igem.org/mediawiki/2017/e/eb/PJET_Cloning.pdf">pJET Cloning</a></p> | <p class="pageText"><a href="https://static.igem.org/mediawiki/2017/e/eb/PJET_Cloning.pdf">pJET Cloning</a></p> | ||
<p class="pageText"><a href="https://static.igem.org/mediawiki/2017/0/08/Plasmid_Miniprep.pdf">Plasmid Miniprep</a></p> | <p class="pageText"><a href="https://static.igem.org/mediawiki/2017/0/08/Plasmid_Miniprep.pdf">Plasmid Miniprep</a></p> |
Revision as of 20:06, 28 October 2017
Protocols
DNA PUrification from Enzymatic Reactions
Phenol/Chloroform Extraction and Ethanol Precipitation
Preparation of Chemical Competent Cells
Purification of Poly-Histidine Tagged Proteins
Restriction Enzyme Digest and Dephosphorylation
Products/Reagents
Enzymes | Buffers/Reagents | Other Products |
---|---|---|
Shrimp Alkaline Phosphatase | Affinity Chromatography Buffers | Ni²+ Affinity Resin |
DNA Blunting Enzyme | IPTG | Prefix Forward: 5’GAATTCGCGGCCGCTTCTAG3’ |
Phusion Polymerase Master Mix | Kanomycin/Chloramphenicol/Ampicillin | Prefix Reverse: 5’CTGCAGCGGCCGCTACTAGTA3’ |
PFU Polymerase | Dimethyl Sulfur Dioxide | Prefix Forward +6:5’GGCTCAGAATTCGCGGCCGCTTCTAGAG3’ |
T7 RNA Polymerase | Competent Cell Buffers | Suffix Reverse +6: 5’CTGGACCTGCAGCGGCCGCTACTAGTA3’ |
T7 DNA Polymerase | T7 RNA Polymerase Purification Buffers | Dialyzation Membrane |
PURExpressKit (New England Biolabs) | Phenol | |
XbaI (NEB) | Chloroform | |
SpeI (NEB) | 10x PFU Buffer | |
PstI (NEB) | NTP | |
EcoRI (NEB) | GMP | |
Dream Taq DNA Polymerase | 10x Taq Buffer | |
Taq DNA Polymerase | Ligase Buffer | |
RNAse A | SDS-PAGE Reagents | |
TraB | Urea PAGE reagents | |
iPPase | Agarose | |
RNAse Inhibitor | Agar | |
DNAseI | Tryptone | |
Yeast Extract | ||
TAKMz | BioBasic Plasmid DNA miniprep Kit | |
Imidazole |