Difference between revisions of "Team:Lethbridge/Parts"
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| − | <p class="text12">The N<i>ex</i>t <i>vivo</i> system is made up of >40 unique parts encoding both protein and RNA components. The main focus of our team this year was to submit all 38 TX-TL protein components to the registry as a collection of parts | + | <p class="text12">The N<i>ex</i>t <i>vivo</i> system is made up of >40 unique parts encoding both protein and RNA components. The main focus of our team this year was to submit all 38 TX-TL protein components to the registry as a <a href="https://2017.igem.org/wiki/index.php?title=Team:Lethbridge/Part_Collection">collection of parts</a> that anyone could use to produce their own N<i>ex</i>t <i>vivo</i> system. Several of these TX-TL components were already in the registry but were under different expression control, lacked purification tags, and/or were not codon optimized for expression in <i>E. coli</i>. As such we sought to <a href="https://2017.igem.org/Team:Lethbridge/Improve">improve these parts</a> (LINK TO IMPROVED PARTS PAGE) for future use. |
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<h2 class="segmentHeader">Part Collection</h2> | <h2 class="segmentHeader">Part Collection</h2> | ||
| − | <p> | + | <p class="text12">The <a href="https://2017.igem.org/wiki/index.php?title=Team:Lethbridge/Part_Collection">N<i>ex</i>t <i>vivo</i> part collection</a> consists of the 38 TX-TL proteins required for RNA transcription and protein synthesis. Each part in the collection has a hexahistidine tag for simultaneous purification of all components, the T7 promoter (BBa_I719005), medium RBS (BBa_B0034), and double terminator (BBa_B0015). |
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Revision as of 20:22, 29 October 2017
The Next vivo system is made up of >40 unique parts encoding both protein and RNA components. The main focus of our team this year was to submit all 38 TX-TL protein components to the registry as a collection of parts that anyone could use to produce their own Next vivo system. Several of these TX-TL components were already in the registry but were under different expression control, lacked purification tags, and/or were not codon optimized for expression in E. coli. As such we sought to improve these parts (LINK TO IMPROVED PARTS PAGE) for future use.
In addition to the TX-TL proteins, we also sought to BioBrick the ribosomal RNA genes and twenty tRNA genes. As a proof of concept for our novel tRNA purification design we only synthesized one representative tRNA gene before ordering them all. Additionally, the Next vivo system makes use of a previously submitted MS2BP part (BBA_K1791003) LINK?!?!?!.
Part Collection
The Next vivo part collection consists of the 38 TX-TL proteins required for RNA transcription and protein synthesis. Each part in the collection has a hexahistidine tag for simultaneous purification of all components, the T7 promoter (BBa_I719005), medium RBS (BBa_B0034), and double terminator (BBa_B0015).
Composite Parts
General Protein Construct
General RNA Construct
Contains all 38 proteins essential for prokaryotic transcription and translation (Tx/Tl) as well 3 additional constructs for tRNA purification and Tx/Tl validation.
Basic Parts
All basic parts pages created on the registry of standard biological parts. Contains all 38 proteins essential for prokaryotic transcription and translation (Tx/Tl) as well as the parts for our tRNA purification and Tx/Tl validation construct.
