Difference between revisions of "Team:Lethbridge/Part Collection"
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<h2 class="segmentHeader">Part Design Overview</h2> | <h2 class="segmentHeader">Part Design Overview</h2> | ||
| − | <p class=" | + | <p class="text14">Each part in our collection was designed with several features in mind:</p> |
| − | <ul class="text12" style="list-style: disc"> | + | <ul class="text12" style="list-style: disc"> |
| − | <li class="text12">Optimized for expression in <i>E. coli</i></li> | + | <li class="text12">Optimized for expression in <i>E. coli</i></li> |
| − | <li class="text12">N- or C-terminal hexahistidine affinity tag</li> | + | <li class="text12">N- or C-terminal hexahistidine affinity tag</li> |
| − | <li class="text12">Constructs contain the T7 promoter (<a href="http://partsregistry.org/Part:BBa_I719005" id="pageLink">BBa_I719005</a>), medium RBS (<a href="http://parts.igem.org/Part:BBa_B0034" id="pageLink">BBa_B0034</a>), and double terminator (<a href="http://parts.igem.org/Part:BBa_B0015" id="pageLink">BBa_B0015</a>)</li> | + | <li class="text12">Constructs contain the T7 promoter (<a href="http://partsregistry.org/Part:BBa_I719005" id="pageLink">BBa_I719005</a>), medium RBS (<a href="http://parts.igem.org/Part:BBa_B0034" id="pageLink">BBa_B0034</a>), and double terminator (<a href="http://parts.igem.org/Part:BBa_B0015" id="pageLink">BBa_B0015</a>)</li> |
</ul> | </ul> | ||
Revision as of 22:25, 30 October 2017
Overview
The Next vivo system is made up of >40 unique parts encoding both protein.
Part Design Overview
Each part in our collection was designed with several features in mind:
- Optimized for expression in E. coli
- N- or C-terminal hexahistidine affinity tag
- Constructs contain the T7 promoter (BBa_I719005), medium RBS (BBa_B0034), and double terminator (BBa_B0015)
Part Collection
The Next vivo system is made up of >40 unique parts encoding both protein and RNA components. The main focus of our team this year was to submit all 38 TX-TL protein components to the registry as a collection of parts that anyone could use to produce their own Next vivo system. Several of these TX-TL components were already in the registry but were under different expression control, lacked purification tags, and/or were not codon optimized for expression in E. coli. As such we sought to improve these parts for future use.
Future Use for iGEM
Our Parts in the Registry
