Difference between revisions of "Team:Lethbridge/Part Collection"

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<p class="text12j">The N<i>ex</i>t <i>vivo</i> system is made up of >40 unique parts encoding both protein.</p>
 
  
 
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Revision as of 01:31, 31 October 2017





Part Design Overview

Each part in our collection was designed with several features in mind:

  • Optimized for expression in E. coli
  • N- or C-terminal hexahistidine affinity tag
  • Constructs contain the T7 promoter (BBa_I719005), medium RBS (BBa_B0034), and double terminator (BBa_B0015)

Part Collection

The Next vivo system is made up of >40 unique parts encoding both protein and RNA components. The main focus of our team this year was to submit all 38 TX-TL protein components to the registry as a collection of parts that anyone could use to produce their own Next vivo system. Several of these TX-TL components were already in the registry but were under different expression control, lacked purification tags, and/or were not codon optimized for expression in E. coli. As such we sought to improve these parts for future use.



Future Use for iGEM



Our Parts in the Registry



<groupparts>iGEM2017 Lethbridge</groupparts>