Difference between revisions of "Team:XJTLU-CHINA/Protocols"
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<a href="https://2017.igem.org/Team:XJTLU-CHINA/Protocols_Ligation"><h3>Ligation</h3></a> | <a href="https://2017.igem.org/Team:XJTLU-CHINA/Protocols_Ligation"><h3>Ligation</h3></a> | ||
<a href="https://2017.igem.org/Team:XJTLU-CHINA/Protocols_Transformation"><h3>Transformation of plasmids in <i>E.coli</i> DH5ɑ</h3></a> | <a href="https://2017.igem.org/Team:XJTLU-CHINA/Protocols_Transformation"><h3>Transformation of plasmids in <i>E.coli</i> DH5ɑ</h3></a> | ||
| − | <a href="https://2017.igem.org/Team:XJTLU-CHINA/Protocols_Isolation"><h3>Isolation of | + | <a href="https://2017.igem.org/Team:XJTLU-CHINA/Protocols_Isolation"><h3>Isolation of plasmids in <i>E.coli</i> DH5ɑ</h3></a> |
<a href="https://2017.igem.org/Team:XJTLU-CHINA/Protocols_Preparation_Competent"><h3>Preparation of competent <i>Lactococcus lactis</i></h3></a> | <a href="https://2017.igem.org/Team:XJTLU-CHINA/Protocols_Preparation_Competent"><h3>Preparation of competent <i>Lactococcus lactis</i></h3></a> | ||
<a href="https://2017.igem.org/Team:XJTLU-CHINA/Protocols_Electroporation"><h3>Electroporation for <i>L. lactis</i></h3></a> | <a href="https://2017.igem.org/Team:XJTLU-CHINA/Protocols_Electroporation"><h3>Electroporation for <i>L. lactis</i></h3></a> | ||
Revision as of 10:27, 26 November 2017
Protocols
Basic Molecular Biology Techniques:
PCR using Q5 Hi-Fi DNA polymerase Master Mix
PCR purification using QIAquick PCR purification kit
Gel extraction
Gibson Assembly
Digestion (NEB enzymes)
Ligation
Transformation of plasmids in E.coli DH5ɑ
Isolation of plasmids in E.coli DH5ɑ
Preparation of competent Lactococcus lactis
Electroporation for L. lactis
Plasmid DNA isolation from Lactococcus lactis
Anti-microbial peptides assay:
Pre-experiment: CFU measurement
Inhibition ring
AMPs efficiency and MIC assay
Biofilm formation test
Growth curve measurement
Nisin induction of gene expression in Lactococcus lactis:
Testing of S. aureus sensing pathway
Autolysis test
Collaborators and Supporters
