Difference between revisions of "Team:Queens Canada"

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<a href="https://2016.igem.org/Team:UofC_Calgary" class="c-logo">                                        
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<h1> Welcome to iGEM 2017! </h1>
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<p>Meow</p>
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<h5>Before you start: </h5>
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<p> Please read the following pages:</p>
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<li>  <a href="https://2017.igem.org/Competition">Competition Hub</a> </li>
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<li> <a href="https://2017.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
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<li> <a href="https://2017.igem.org/Resources/Template_Documentation">Template documentation</a></li>
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<h3 class="c-center c-font-uppercase c-font-bold" style="color:#EBDEBE">Giant Jamboree Results</h3>
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<h5> Project Description </h5>
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<p>Biofilms are often maligned because of their roles in antibiotic-resistant infections and dental plaque. However, biofilms also offer an attractive platform for the design of self- assembling biomaterials programmed for specific functionality. The amyloid protein CsgA accounts for the majority of the proteinaceous component, curli nanofibres, of <em>Escherichia coli</em> biofilms. CsgA has been shown to be relatively tolerant of C-terminal fusions, allowing CsgA endowed with diverse peptide domains to be secreted and self-assembled outside the cell similar to normal curli nanofibres. Here, QGEM presents the genetic engineering of CsgA to create a bifunctional biofilm that both binds ice and degrades hydrocarbons. A type I antifreeze protein, AFP8, will be fused to CsgA for ice binding, and a PA-14 adhesin domain will be fused, via the SpyTag-SpyCatcher system, to bind the hydrocarbon-degrading bacterium <em>Marinobacter hydrocarbonoclasticus</em>. Thus, the end-product will be a bifunctional biofilm with the capacity to establish itself on Arctic ice for the purpose of degrading toxic hydrocarbons present in oil spills. Our modelling team will be compiling a code that calculates optimal promoter strengths for a desired biofilm composition (e.g. antifreeze protein to adhesin Csg ratio), with the ultimate goal of using logic gates to design responsive biofilms that can alter functionality when exposed to different environmental cues.</p>  
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                                            <p style="color: #FFFFFF">We are honoured to announce that the UofC_Calgary 2016 iGEM was incredibly successful at this year's iGEM Giant Jamboree. We received a <u>gold medal</u>, a nomination for <u>Best Applied Design</u>, and the award for <u>Best Integrated Human Practices</u>! We are beyond thrilled to continue the University of Calgary's tradition of excellence at iGEM, and extend our deepest gratitude to those who aided us in any form during this journey.
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</p><div class="col-md-4"><center><img src="https://static.igem.org/mediawiki/2016/a/a3/T--UofC_Calgary--Gold%28%2B%29.png" style="width:20vw;" alt="Gold Medal didn't load. Please Refresh Page."></center></div><div class="col-md-8" style="padding-top: 50px;"><center> <img width="500px" height="375px" src="https://static.igem.org/mediawiki/2016/5/5a/T--UofC_Calgary--boston_photo.jpg"></center>
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<h5> Wiki template information </h5>
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                            <h3 class="c-center c-font-uppercase c-font-bold" style="color:#EBDEBE">Our Project</h3>
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2017.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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                                            <p style="color: #FFFFFF">Mars and the cosmos are a tantalizingly close goal for Mankind to reach in the next century; however, there are several glaring problems that we must overcome to reach such an objective. Radiation is chief among them. On Earth, individuals annually receive 2.4 mSv of ionizing radiation (IR), which is easily tolerated by the human body. Unfortunately, without the protection of the magnetosphere, astronauts in space are exposed to high levels of IR in the range of 50 to 2000 mSv. This level of radiation causes the accumulation of deleterious double strand breaks in DNA. Despite current research into methods of IR protection, many solutions such as radiation shield coating are quite expensive, especially to transport to space. Even existing biological solutions are insufficient, such as the use of ingestible or injectable radioprotectors, which are subject to sinusuoidal pharmacokinetics. Our project is based on the administration of the naturally occuring peptide Bowman-Birk Protease Inhibitor (BBI), which has been shown to confer protection against DNA damage following radiation exposure. (Dittmann <i>et al.</i>, 2003). </p>
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<p style="color: #FFFFFF">References</p>
 
  
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<p style="color: #FFFFFF"><font size="2">Dittmann, K.H., Mayer, C., and Rodemann, H.P. (2003). Radioprotection of normal tissue to improve radiotherapy: the effect of the Bowman Birk protease inhibitor. <i>Current Medicinal Chemistry - Anticancer Agents, 3</i>(5), 360-363.</font </p>
 
  
  
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<h5> Editing your wiki </h5>
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
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<p> <a href="https://2017.igem.org/wiki/index.php?title=Team:Example&action=edit"> </a>Use WikiTools - Edit in the black menu bar to edit this page</p>
  
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                            <h3 class="c-center c-font-uppercase c-font-bold" style="color:#273236 !important">OUR CHASSIS</h3>
 
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                                            <p style="color: #273236">To express mBBI, we had to choose a biological system that was not only capable of high expression but was robust, adaptable, and easily genetically manipulated for ease of transport and use in remote and extreme environments. After creating a list of different types of cells which could be used for the expression of our peptide, we found that <i>Bacillus subtilis</i> was especially suited for our patch design. This is due to many reasons. <i>B. subtilis</i> is able to express high amounts of recombinant protein which can be secreted into surrounding media by its own well-characterized secretion system, making it ideal for producing and delivering the correct dosage of mBBI into the user. <i>B. subtilis</i> is capable of forming spores—which have been found in arctic permafrost dating tens of thousands of years and yet are still viable—making it easy to store in a desiccated or frozen manner. Additionally, <i>B. subtilis</i> expresses high amounts of recombinase A, a protein which allows genes to be stably integrated into the chromosome.</p>
 
  
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<p style="color: #273236">Our strain of <i>B. subtilis</i> (WB800) is particularly suited for high production because it is deficient in eight extracellular proteases which could lead to the premature degradation our radioprotective peptide. Our strain will allow the peptide to be stable in the media within the patch. We conducted tests on the growth patterns of <i>B. subtilis</i> in the novel environment of a patch and optimized patch media to simulate an ideal growth conditions. We have also designed a genetic construct which provides inducible competency to the <i>B. subtilis</i> strain upon the addition of xylose, making this bacterium much easier to work with for future teams. </p>  
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<h5>Tips</h5>
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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<li>Be clear about what you are doing and how you plan to do this.</li>
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2017.igem.org/Calendar">iGEM 2017 calendar</a> </li>
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<li>Have lots of fun! </li>
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                                        <br> <br> <br> <br> <br><p style="color: #FFFFFF">We designed our project to overcome common issues in existing technologies such as cost, portability, and durability. Our patch is portable, lightweight, safe to use, can be stored long-term, and produces and delivers peptides at a steady rate to maintain a constant concentration in the body. We worked with professionals within NASA and the CSA to integrate our solution with current space travel infrastructure, and with companies specializing in transdermal delivery to optimize the patch system. We also consulted with professionals and researchers in biomedical fields to better understand the unknown effects our delivery system would have when in use. Using this system, our project has future applications here on Earth for other occupations and individuals exposed to high levels of ionizing radiation, such as pilots, flight attendants, and miners. Our project can also be used to deliver hormones and other small molecules for use in space, serving as a "biological pharmacy." </p> <br> <br> <br> <br> <br> <br> <br> <br> <br>
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<h5>Inspiration</h5>
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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        <a href="https://2016.igem.org/Team:UofC_Calgary/Policy">
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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                                  <a href="https://2016.igem.org/Team:UofC_Calgary/Experiments"><img class="box-img" src="https://static.igem.org/mediawiki/2016/4/4b/T--UofC_Calgary--chassis.jpg"> </a></img> <p style="color:#FFFFFF; text-align: center; font-size: 16px;"> Our Experiments </p>     
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Transdermal Microneedle Drug Delivery Patch
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NOSHIN
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3M Microneedles
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The 2016 U of C Calgary iGEM team is a multidisciplinary team based out of the University of Calgary. Our team is made up of undergraduate students from all years of study, hailing from the broad backgrounds of biology, microbiology, biomedical sciences, bioinformatics, and engineering. We are based out of the O'Brien Centre Labs within the U of C's Health Science Centre.
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<a href="http://www.ucalgary.ca/"><img class="c-img-pos" src="https://static.igem.org/mediawiki/2016/3/35/T--UofC_Calgary--uofc.png" alt="" style="width: 180px; height:140px;"></a>
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<a href="https://www.mindfuel.ca/?gclid=CJ_GjpW0384CFZSIfgodOW4F0A"><img class="c-img-pos" src="https://static.igem.org/mediawiki/2016/1/1f/T--UofC_Calgary--mindfuel.png" alt="" style="width: 180px; height:140px;"></a>
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<a href="http://www.albertatechfutures.ca/Research/BasicResearch/geekStarter.aspx"><img class="c-img-pos" src="https://static.igem.org/mediawiki/2016/7/7d/T--UofC_Calgary--geekstarter.png" alt="" style="width: 180px; height:140px;"></a>
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<a href="http://store.biobasic.com/"><img class="c-img-pos" src="https://static.igem.org/mediawiki/2016/a/a6/T--UofC_Calgary--biobasic.png" alt="" style="width: 180px; height:140px;"></a>
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<h5> Uploading pictures and files </h5>
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<p> You can upload your pictures and files to the iGEM 2017 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
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When you upload, set the "Destination Filename" to <br><code>T--YourOfficialTeamName--NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)<br><br>
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UPLOAD FILES
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<h3 class="c-font-bold c-font-white">i<span class="c-theme-font">GEM</span></h3>
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<div class="c-line-left hide">
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<p class="c-text">
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iGEM is an international competition promoting synthetic biology as a means to solve social, economic and humanitarian problems around the globe. The iGEM Jamboree is held in Boston annually. In 2016, over 300 teams are competing against each other.
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<h3 class="c-font-uppercase c-font-bold c-font-white">Latest Entries</h3>
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<h4 class="c-post-title"><a href="#">Fully Trained!</a></h4>
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Our entire team received a full BioSafety education from the University of Calgary! This entailed going to classes to prepare for a final quiz that tested our ability to be safe in the lab. Several of our members also had radiation training and clearance to ensure that work done with radiation was safe!
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<a href="#" class="btn btn-md c-btn-border-1x c-theme-btn c-btn-uppercase c-btn-square c-btn-bold c-read-more hide">Read More</a>
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<a href="https://www.instagram.com/igemcalgary/"><img src="https://static.igem.org/mediawiki/2016/2/2a/T--UofC_Calgary--wikiinsat2.jpg" class="img-responsive" alt=""/></a>
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<a href="#" class="btn btn-md c-btn-border-1x c-theme-btn c-btn-uppercase c-btn-square c-btn-bold c-read-more hide">View More</a>
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Located in Calgary, Alberta, Canada.
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<i class="icon-pointer c-theme-font"></i> University of Calgary
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<i class="icon-envelope c-theme-font"></i> igem.calgary@gmail.com
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Copyright &copy; University of Calgary 2016.
 
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Revision as of 17:19, 31 July 2017