Difference between revisions of "Team:Lethbridge/Improve"

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                     <h2>Part BBa_K1791001</h2>
 
                     <h2>Part BBa_K1791001</h2>
 
<p>Part BBa_K1791001 contains two modules, a standard ribosome binding site (RBS) expressing MS2 coat protein and a theophylline ribozyme with a MS2 aptamer at the 3’ end. To express MS2 coat  protein the part utilizes expression of c-terminal 6x His tagged MS2 coat protein under the regulation of  a T7 promoter and a standard Shine Dalgarno sequence followed by a double terminator (BBa_0015).  Downstream of the RBS is a T7 promoter followed by a theophylline ribozyme and a MS2 aptamer at the  3’ end. Coexpression of the two modules results in MS2 binding to the 3’ end of the theophylline ribozyme module which allows for MS2-RNA purification by nickel affinity chromatography, RNA can  then be eluted from the MS2-RNA complex by addition of theophylline.  </p>
 
<p>Part BBa_K1791001 contains two modules, a standard ribosome binding site (RBS) expressing MS2 coat protein and a theophylline ribozyme with a MS2 aptamer at the 3’ end. To express MS2 coat  protein the part utilizes expression of c-terminal 6x His tagged MS2 coat protein under the regulation of  a T7 promoter and a standard Shine Dalgarno sequence followed by a double terminator (BBa_0015).  Downstream of the RBS is a T7 promoter followed by a theophylline ribozyme and a MS2 aptamer at the  3’ end. Coexpression of the two modules results in MS2 binding to the 3’ end of the theophylline ribozyme module which allows for MS2-RNA purification by nickel affinity chromatography, RNA can  then be eluted from the MS2-RNA complex by addition of theophylline.  </p>
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<img src="https://static.igem.org/mediawiki/2015/8/84/Uleth15_StandardRPA.png" height="30%" width="30%">
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<p>Check it out on the <a href="http://parts.igem.org/Part:BBa_K1791001">Registry</a></p>
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                     <h2>Part BBa_K1791002</h2>
 
                     <h2>Part BBa_K1791002</h2>
 
<p>Part BBa_K17910012 contains two modules, a low efficiency ribosome binding site (RBS)  expressing MS2 coat protein and a theophylline ribozyme with a MS2 aptamer at the 3’ end. To express  MS2 coat protein the part utilizes expression of c-terminal 6x His tagged MS2 coat protein under a T7  promoter and a standard Shine Dalgarno sequence followed by a double terminator (BBa_0015).  Downstream of the RBS is a T7 promoter followed by a theophylline ribozyme and a MS2 aptamer at the  3’ end. Coexpression of the two modules results in MS2 binding to the 3’ end of the theophylline  ribozyme construct which allows for MS2-RNA purification by nickel affinity chromatography, RNA can  then be eluted from the MS2-RNA complex by addition of theophylline.</p>
 
<p>Part BBa_K17910012 contains two modules, a low efficiency ribosome binding site (RBS)  expressing MS2 coat protein and a theophylline ribozyme with a MS2 aptamer at the 3’ end. To express  MS2 coat protein the part utilizes expression of c-terminal 6x His tagged MS2 coat protein under a T7  promoter and a standard Shine Dalgarno sequence followed by a double terminator (BBa_0015).  Downstream of the RBS is a T7 promoter followed by a theophylline ribozyme and a MS2 aptamer at the  3’ end. Coexpression of the two modules results in MS2 binding to the 3’ end of the theophylline  ribozyme construct which allows for MS2-RNA purification by nickel affinity chromatography, RNA can  then be eluted from the MS2-RNA complex by addition of theophylline.</p>
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<img src="https://static.igem.org/mediawiki/2015/e/e5/Uleth_ConstructSchematic.png" height="30%" width="30%">
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<p>Check it out on the <a href="http://parts.igem.org/Part:BBa_K1791002">Registry</a></p>
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                 </div>
 
                 </div>

Revision as of 05:36, 19 October 2017

Composite Parts

Part BBa_K1791001

Part BBa_K1791001 contains two modules, a standard ribosome binding site (RBS) expressing MS2 coat protein and a theophylline ribozyme with a MS2 aptamer at the 3’ end. To express MS2 coat protein the part utilizes expression of c-terminal 6x His tagged MS2 coat protein under the regulation of a T7 promoter and a standard Shine Dalgarno sequence followed by a double terminator (BBa_0015). Downstream of the RBS is a T7 promoter followed by a theophylline ribozyme and a MS2 aptamer at the 3’ end. Coexpression of the two modules results in MS2 binding to the 3’ end of the theophylline ribozyme module which allows for MS2-RNA purification by nickel affinity chromatography, RNA can then be eluted from the MS2-RNA complex by addition of theophylline.


Part BBa_K1791002

Part BBa_K17910012 contains two modules, a low efficiency ribosome binding site (RBS) expressing MS2 coat protein and a theophylline ribozyme with a MS2 aptamer at the 3’ end. To express MS2 coat protein the part utilizes expression of c-terminal 6x His tagged MS2 coat protein under a T7 promoter and a standard Shine Dalgarno sequence followed by a double terminator (BBa_0015). Downstream of the RBS is a T7 promoter followed by a theophylline ribozyme and a MS2 aptamer at the 3’ end. Coexpression of the two modules results in MS2 binding to the 3’ end of the theophylline ribozyme construct which allows for MS2-RNA purification by nickel affinity chromatography, RNA can then be eluted from the MS2-RNA complex by addition of theophylline.