Difference between revisions of "Team:Lethbridge/Improve"

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                 <div class="content_box">
 
                 <div class="content_box">
 
      
 
      
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                     <h1>Composite Parts</h1>
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                     <h1>Improved Parts</h1>
 
      
 
      
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                     <h2>Part BBa_K1791001</h2>
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                     <h2>BBa_K2443037 - T7 RNA Polymerase</h2>
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<p>Part BBa_K1791001 contains two modules, a standard ribosome binding site (RBS) expressing MS2 coat protein and a theophylline ribozyme with a MS2 aptamer at the 3’ end. To express MS2 coat  protein the part utilizes expression of c-terminal 6x His tagged MS2 coat protein under the regulation of  a T7 promoter and a standard Shine Dalgarno sequence followed by a double terminator (BBa_0015). Downstream of the RBS is a T7 promoter followed by a theophylline ribozyme and a MS2 aptamer at the  3’ end. Coexpression of the two modules results in MS2 binding to the 3’ end of the theophylline ribozyme module which allows for MS2-RNA purification by nickel affinity chromatography, RNA can  then be eluted from the MS2-RNA complex by addition of theophylline. </p>
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<p>Original part: BBa_I2032</p>
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<p>Submitted by MIT in 2006 and designed by: Bartholomew Canton</p>
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<p>Encodes only for the coding region of T7 RNA polymerase. In order to improve it and incorporate it into our Next<i>Vivo</i> we have codon optimized it and attached a 6 X His tag for easy purification. </P>  
  
  

Revision as of 05:44, 19 October 2017

Improved Parts

BBa_K2443037 - T7 RNA Polymerase

Original part: BBa_I2032

Submitted by MIT in 2006 and designed by: Bartholomew Canton

Encodes only for the coding region of T7 RNA polymerase. In order to improve it and incorporate it into our NextVivo we have codon optimized it and attached a 6 X His tag for easy purification.


Part BBa_K1791002

Part BBa_K17910012 contains two modules, a low efficiency ribosome binding site (RBS) expressing MS2 coat protein and a theophylline ribozyme with a MS2 aptamer at the 3’ end. To express MS2 coat protein the part utilizes expression of c-terminal 6x His tagged MS2 coat protein under a T7 promoter and a standard Shine Dalgarno sequence followed by a double terminator (BBa_0015). Downstream of the RBS is a T7 promoter followed by a theophylline ribozyme and a MS2 aptamer at the 3’ end. Coexpression of the two modules results in MS2 binding to the 3’ end of the theophylline ribozyme construct which allows for MS2-RNA purification by nickel affinity chromatography, RNA can then be eluted from the MS2-RNA complex by addition of theophylline.