Difference between revisions of "Team:Lethbridge/Improve"
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<h2>BBa_K2443037 - T7 RNA Polymerase</h2> | <h2>BBa_K2443037 - T7 RNA Polymerase</h2> | ||
| − | <p>Original part: BBa_I2032</p> | + | <p><b>Original part: BBa_I2032</b></p> |
| − | <p>Submitted by MIT in 2006 and designed by: Bartholomew Canton</p> | + | <p><b>Submitted by:</b> MIT in 2006 and <b>designed by:<b/> Bartholomew Canton</p> |
<p>Encodes only for the coding region of T7 RNA polymerase. In order to improve it and incorporate it into our Next<i>Vivo</i> system we have codon optimized it and attached a 6 X His tag for easy purification. </P> | <p>Encodes only for the coding region of T7 RNA polymerase. In order to improve it and incorporate it into our Next<i>Vivo</i> system we have codon optimized it and attached a 6 X His tag for easy purification. </P> | ||
Revision as of 05:45, 19 October 2017
Improved Parts
BBa_K2443037 - T7 RNA Polymerase
Original part: BBa_I2032
Submitted by: MIT in 2006 and designed by:<b/> Bartholomew Canton</p>
<p>Encodes only for the coding region of T7 RNA polymerase. In order to improve it and incorporate it into our NextVivo system we have codon optimized it and attached a 6 X His tag for easy purification. </P>
Part BBa_K1791002
<p>Part BBa_K17910012 contains two modules, a low efficiency ribosome binding site (RBS) expressing MS2 coat protein and a theophylline ribozyme with a MS2 aptamer at the 3’ end. To express MS2 coat protein the part utilizes expression of c-terminal 6x His tagged MS2 coat protein under a T7 promoter and a standard Shine Dalgarno sequence followed by a double terminator (BBa_0015). Downstream of the RBS is a T7 promoter followed by a theophylline ribozyme and a MS2 aptamer at the 3’ end. Coexpression of the two modules results in MS2 binding to the 3’ end of the theophylline ribozyme construct which allows for MS2-RNA purification by nickel affinity chromatography, RNA can then be eluted from the MS2-RNA complex by addition of theophylline.</p>
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