Difference between revisions of "Team:Lethbridge/Improve"
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<h2>BBa_K2443037 - T7 RNA Polymerase</h2> | <h2>BBa_K2443037 - T7 RNA Polymerase</h2> | ||
| + | <p><b>Original part:</b> BBa_I2032</p> | ||
| + | <p><b>Submitted by:</b> MIT in 2006</p> | ||
| + | <p><b>designed by:</b> Bartholomew Canton</p> | ||
| + | <p><b>Rational behind improvements:</b></p> | ||
| + | <p>BBa_I2032 encodes exclusively for the coding region of T7 RNA polymerase. In order to improve it and incorporate it into our Next<i>Vivo</i> system we have Codon optimized for use in <i>Escherichia coli</i> and attached a N-terminal 6 X His tag with a serine glycine linker for easy purification. We have also optimized our T7 RNA polymerase to be overexpressed in BL21DE3 gold cells by putting it under the control of a T7 promoter (BBa_I719005), RBS (BBa_B0034) and double terminator (BBa_B0015)</P> | ||
| + | |||
| + | <h2>BBa_K2443037 - T7 RNA Polymerase</h2> | ||
<p><b>Original part:</b> BBa_I2032</p> | <p><b>Original part:</b> BBa_I2032</p> | ||
<p><b>Submitted by:</b> MIT in 2006</p> | <p><b>Submitted by:</b> MIT in 2006</p> | ||
Revision as of 05:57, 19 October 2017
Improved Parts
BBa_K2443037 - T7 RNA Polymerase
Original part: BBa_I2032
Submitted by: MIT in 2006
designed by: Bartholomew Canton
Rational behind improvements:
BBa_I2032 encodes exclusively for the coding region of T7 RNA polymerase. In order to improve it and incorporate it into our NextVivo system we have Codon optimized for use in Escherichia coli and attached a N-terminal 6 X His tag with a serine glycine linker for easy purification. We have also optimized our T7 RNA polymerase to be overexpressed in BL21DE3 gold cells by putting it under the control of a T7 promoter (BBa_I719005), RBS (BBa_B0034) and double terminator (BBa_B0015)
BBa_K2443037 - T7 RNA Polymerase
Original part: BBa_I2032
Submitted by: MIT in 2006
designed by: Bartholomew Canton
Rational behind improvements:
BBa_I2032 encodes exclusively for the coding region of T7 RNA polymerase. In order to improve it and incorporate it into our NextVivo system we have Codon optimized for use in Escherichia coli and attached a N-terminal 6 X His tag with a serine glycine linker for easy purification. We have also optimized our T7 RNA polymerase to be overexpressed in BL21DE3 gold cells by putting it under the control of a T7 promoter (BBa_I719005), RBS (BBa_B0034) and double terminator (BBa_B0015)
