Difference between revisions of "Team:Manchester/Improve"
| Line 64: | Line 64: | ||
<p><i> Please visit our <a href="https://2017.igem.org/Team:Manchester/Part_Collection"><b>Part Collection</b></a> page for ALL PARTS.</i></p> | <p><i> Please visit our <a href="https://2017.igem.org/Team:Manchester/Part_Collection"><b>Part Collection</b></a> page for ALL PARTS.</i></p> | ||
| − | |||
<p> The Manchester iGEM 2017 team have improved part <a href="http://partsregistry.org/Part:BBa_K562001"><b>BBa_K562001</b></a>, originally submitted by team <a href="https://2011.igem.org/Team:Dundee"></b>Dundee 2011</b></a>.</p> | <p> The Manchester iGEM 2017 team have improved part <a href="http://partsregistry.org/Part:BBa_K562001"><b>BBa_K562001</b></a>, originally submitted by team <a href="https://2011.igem.org/Team:Dundee"></b>Dundee 2011</b></a>.</p> | ||
Revision as of 20:04, 22 October 2017
Improved Parts
Please visit our Part Collection page for ALL PARTS.
The Manchester iGEM 2017 team have improved part BBa_K562001, originally submitted by team Dundee 2011.
We have removed the illegal XbaI site at 179 in BBa_K562001 to make it biobrick compatible.
We have also further improved this part by adding different Anderson promoters (Low BBa_J23105, Medium BBa_J23108, High BBa_J23104) to control the expression level of the localisation tag. This enables optimisation of localisation for specific purposes. For example, when targeting a particularly large protein, it may be advantageous to use a lowly expressing promoter to prevent any disturbance to micro-compartment formation.
| Part Name | BioBrick | Description | Part Type |
|---|---|---|---|
| LowPromoter_PduD(1-20)_mCherry | BBa_K2213006 | Part Description | Basic |
| MediumPromoter_PduD(1-20)_mCherry | BBa_K2213007 | Part Description. | Basic |
| HighPromoter_PduD(1-20)_mCherry | BBa_K2213008 | Part Description. | Basic |
