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+ | <meta charset="UTF-8"> | ||
+ | <title>A F C M</title> | ||
+ | <meta name="description" content=""> | ||
+ | <meta name="author" content="Web Domus Italia"> | ||
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+ | <body> | ||
+ | <center><span style="color: #ffffff; font-size: 300%;"><span style="text-decoration: underline;">Parts</span><br /><br /></span></center><center><span style="color: #ffffff; font-size: x-large;">We submitted the basic parts constituting our gene fragments, then<br /> we designed the composite parts constituting these synthesised fragments<br /> and finally we assembled these composite parts into our two main circuits<br /> designed in order to compare their function at regulating non-coding RNAs.</span></center> | ||
+ | <p style="text-align: center;"></p> | ||
+ | <center><span style="color: #000000; font-size: 300%;"><span style="text-decoration: underline;"><br /><span style="color: #ffffff; text-decoration: underline;">Basic Parts</span></span><br /><br /></span></center> | ||
+ | <table style="margin-left: auto; margin-right: auto;"> | ||
+ | <tbody> | ||
+ | <tr> | ||
+ | <th>Part Name</th> | ||
+ | <th>Type</th> | ||
+ | <th style="text-align: center;">Description</th> | ||
+ | <th>Length</th> | ||
+ | <th>Link</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>BBa_K2217000</td> | ||
+ | <td>Basic</td> | ||
+ | <td>CMV Enhancer</td> | ||
+ | <td>304</td> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2217000">HERE</a></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>BBa_K2217001</td> | ||
+ | <td>Basic</td> | ||
+ | <td>Circular RNA hsa-circ-000064</td> | ||
+ | <td>139</td> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2217001">HERE</a></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>BBa_K2217002</td> | ||
+ | <td>Basic</td> | ||
+ | <td>U6 Promoter for Cas9 gRNA</td> | ||
+ | <td>204</td> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2217002">HERE</a></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>BBa_K2217003</td> | ||
+ | <td>Basic</td> | ||
+ | <td>gRNA for Cas9 Knock in</td> | ||
+ | <td>20</td> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2217003">HERE</a></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>BBa_K2217004</td> | ||
+ | <td>Basic</td> | ||
+ | <td>gRNA scaffold for Cas9</td> | ||
+ | <td>76</td> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2217004">HERE</a></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>BBa_K2217005</td> | ||
+ | <td>Basic</td> | ||
+ | <td>SV40 Terminator</td> | ||
+ | <td>122</td> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2217005">HERE</a></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>BBa_K2217006</td> | ||
+ | <td>Basic</td> | ||
+ | <td>CMV Promoter</td> | ||
+ | <td>204</td> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2217006">HERE</a></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>BBa_K2217007</td> | ||
+ | <td>Basic</td> | ||
+ | <td>T7 Promoter</td> | ||
+ | <td>19</td> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2217007">HERE</a></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>BBa_K2217008</td> | ||
+ | <td>Basic</td> | ||
+ | <td>CYC1 Terminator</td> | ||
+ | <td>250</td> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2217008">HERE</a></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>BBa_K2217009</td> | ||
+ | <td>Basic</td> | ||
+ | <td>HDR Template of BBa_K2217001</td> | ||
+ | <td>139</td> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2217009">HERE</a></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>BBa_K2217010</td> | ||
+ | <td>Basic</td> | ||
+ | <td>Chicken B-actin Promoter</td> | ||
+ | <td>278</td> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2217010">HERE</a></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>BBa_K2217012</td> | ||
+ | <td>Basic</td> | ||
+ | <td>laci</td> | ||
+ | <td>1080</td> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2217012">HERE</a></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>BBa_K2217014</td> | ||
+ | <td>Basic</td> | ||
+ | <td>YFP</td> | ||
+ | <td>720</td> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2217014">HERE</a></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>BBa_K2217015</td> | ||
+ | <td>Basic</td> | ||
+ | <td>bGH poly(A) signal</td> | ||
+ | <td>225</td> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2217015">HERE</a></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>BBa_K2217016</td> | ||
+ | <td>Basic</td> | ||
+ | <td>lac_operator</td> | ||
+ | <td>17</td> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2217016">HERE</a></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>BBa_K2217017</td> | ||
+ | <td>Basic</td> | ||
+ | <td>lac_promoter</td> | ||
+ | <td>31</td> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2217017">HERE</a></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>BBa_K2217021</td> | ||
+ | <td>Basic</td> | ||
+ | <td>miRNA-1825 binding site on hsa-circ-00064</td> | ||
+ | <td>20</td> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K2217021">HERE</a></td> | ||
+ | </tr> | ||
+ | </tbody> | ||
+ | </table> | ||
+ | <p><br /><br /><br /><br /><br /><br /></p> | ||
+ | </body> | ||
</html> | </html> | ||
+ | {{Template:AFCM-Egypt/footer}} |
Revision as of 16:09, 26 October 2017
we designed the composite parts constituting these synthesised fragments
and finally we assembled these composite parts into our two main circuits
designed in order to compare their function at regulating non-coding RNAs.
Basic Parts
Part Name | Type | Description | Length | Link |
---|---|---|---|---|
BBa_K2217000 | Basic | CMV Enhancer | 304 | HERE |
BBa_K2217001 | Basic | Circular RNA hsa-circ-000064 | 139 | HERE |
BBa_K2217002 | Basic | U6 Promoter for Cas9 gRNA | 204 | HERE |
BBa_K2217003 | Basic | gRNA for Cas9 Knock in | 20 | HERE |
BBa_K2217004 | Basic | gRNA scaffold for Cas9 | 76 | HERE |
BBa_K2217005 | Basic | SV40 Terminator | 122 | HERE |
BBa_K2217006 | Basic | CMV Promoter | 204 | HERE |
BBa_K2217007 | Basic | T7 Promoter | 19 | HERE |
BBa_K2217008 | Basic | CYC1 Terminator | 250 | HERE |
BBa_K2217009 | Basic | HDR Template of BBa_K2217001 | 139 | HERE |
BBa_K2217010 | Basic | Chicken B-actin Promoter | 278 | HERE |
BBa_K2217012 | Basic | laci | 1080 | HERE |
BBa_K2217014 | Basic | YFP | 720 | HERE |
BBa_K2217015 | Basic | bGH poly(A) signal | 225 | HERE |
BBa_K2217016 | Basic | lac_operator | 17 | HERE |
BBa_K2217017 | Basic | lac_promoter | 31 | HERE |
BBa_K2217021 | Basic | miRNA-1825 binding site on hsa-circ-00064 | 20 | HERE |