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− | {{AFCM-Egypt}} | + | {{Template:AFCM-Egypt/bootstrap}} |
+ | {{Template:AFCM-Egypt/main}} | ||
+ | {{Template:AFCM-Egypt/header}} | ||
<html> | <html> | ||
+ | <head> | ||
+ | <meta charset="UTF-8"> | ||
+ | <title>A F C M</title> | ||
+ | <meta name="description" content=""> | ||
+ | <meta name="author" content="Web Domus Italia"> | ||
+ | <meta http-equiv="X-UA-Compatible" content="IE=edge,chrome=1"> | ||
+ | <meta name="viewport" content="width=device-width, initial-scale=1.0"> | ||
+ | <style> | ||
+ | .PARTS {border: 1px solid #fff; | ||
+ | background: rgba(255,255,255, 0.3);} | ||
+ | table { | ||
+ | font-family: arial, sans-serif; | ||
+ | font-color: #fff | ||
+ | border-collapse: collapse; | ||
+ | width: Auto; | ||
+ | background-color: #fff; | ||
+ | } | ||
− | + | td, th { | |
− | + | border: 1px solid #dddddd; | |
− | + | text-align: left; | |
− | + | padding: 8px; | |
− | + | } | |
− | + | ||
+ | tr:nth-child(even) { | ||
+ | background-color: #eca808; | ||
+ | font-color: #fff | ||
+ | } | ||
+ | </style> | ||
+ | </head> | ||
+ | <body> | ||
+ | <p style="text-align: center;"></p> | ||
+ | <table style="width: 842px; border-color: #e01010; margin-left: auto; margin-right: auto;" align="center"> | ||
+ | <tbody> | ||
+ | <tr> | ||
+ | <th style="width: 110px;">Part Name</th> | ||
+ | <th style="width: 451px;">Description</th> | ||
+ | <th style="width: 54px;">Length</th> | ||
+ | <th style="width: 153px;">Link</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td style="width: 110px;">BBa_K2217019</td> | ||
+ | <td style="width: 451px;">Cas9 Knock-in</td> | ||
+ | <td style="width: 54px;">6035</td> | ||
+ | <td style="width: 153px;"><a href="http://parts.igem.org/Part:BBa_K2217019">HERE</a></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td style="width: 110px;">BBa_K2217020</td> | ||
+ | <td style="width: 451px;">BBa_K2217001 ceRNA hsa-circ-0000064</td> | ||
+ | <td style="width: 54px;">867</td> | ||
+ | <td style="width: 153px;"><a href="http://parts.igem.org/Part:BBa_K2217020">HERE</a></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td style="width: 110px;">BBa_K2217022</td> | ||
+ | <td style="width: 451px;">laci + miRNA-1825 binding site on hsa-circ-0000064 Production</td> | ||
+ | <td style="width: 54px;">1732d</td> | ||
+ | <td style="width: 153px;"><a href="http://parts.igem.org/Part:BBa_K2217022">HERE</a></td> | ||
+ | </tr> | ||
+ | </tbody> | ||
+ | </table> | ||
+ | <p><br /><br /><br /><br /></p> | ||
+ | <center> | ||
+ | <span style="color: #ffffff; font-size: xx-large;"> | ||
+ | Composite Parts representing Synthesized <br /> | ||
+ | Fragments | ||
+ | </span> | ||
+ | </center> | ||
− | <div class=" | + | <div class="col-md-6 text-center" style="float:none;margin:auto"> |
− | < | + | <span style="color: #000000; font-size: x-large;"> |
− | + | <span style="color: #ffffff;"> | |
− | + | <br /> | |
− | + | These composite parts were synthesised as separate gene fragments then assembled to form | |
− | + | </span> | |
− | </ | + | <span style="color: #ff9900;">BBa_K2217025 |
− | + | </span> | |
− | + | <span style="color: #ffffff;">and</span> | |
− | + | <span style="color: #ff9900;">BBa_K2217026</span> | |
− | < | + | <span style="color: #ffffff;"> representing ceRNA and CRISPR circuits respectively.</span><br /><br /><br /> |
− | + | </span> | |
− | + | ||
− | + | ||
− | + | ||
− | < | + | |
− | + | ||
− | <br | + | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
</div> | </div> | ||
− | + | <table style="border-color: #b80b0b; margin-left: auto; margin-right: auto;" align="center"> | |
− | </ | + | <tbody> |
− | + | <tr style="height: 18px;"> | |
+ | <th style="height: 18px; width: 110px;">Part Name</th> | ||
+ | <th style="height: 18px; width: 547px;">Description</th> | ||
+ | <th style="height: 18px; width: 54px;">Length</th> | ||
+ | <th style="height: 18px; width: 39px;">Link</th> | ||
+ | </tr> | ||
+ | <tr style="height: 18px;"> | ||
+ | <td style="height: 18px; width: 110px;">BBa_K2217024</td> | ||
+ | <td style="height: 18px; width: 547px;">CMV Enhancer + CMV Promoter + T7</td> | ||
+ | <td style="height: 18px; width: 54px;">542</td> | ||
+ | <td style="height: 18px; width: 39px;"><a href="http://parts.igem.org/Part:BBa_K2217024">HERE</a></td> | ||
+ | </tr> | ||
+ | <tr style="height: 18px;"> | ||
+ | <td style="height: 18px; width: 110px;">BBa_K2217027</td> | ||
+ | <td style="height: 18px; width: 547px;">laci + miRNA-1825 binding site synthesized fragment</td> | ||
+ | <td style="height: 18px; width: 54px;">1210</td> | ||
+ | <td style="height: 18px; width: 39px;"><a href="http://parts.igem.org/Part:BBa_K2217027">HERE</a></td> | ||
+ | </tr> | ||
+ | <tr style="height: 18px;"> | ||
+ | <td style="height: 18px; width: 110px;">BBa_K2217028</td> | ||
+ | <td style="height: 18px; width: 547px;">BBa_K2217001 ceRNA hsa-circ-0000064 + sv_poly(A) Synthesized Fragment</td> | ||
+ | <td style="height: 18px; width: 54px;">269</td> | ||
+ | <td style="height: 18px; width: 39px;"><a href="http://parts.igem.org/Part:BBa_K2217028">HERE</a></td> | ||
+ | </tr> | ||
+ | <tr style="height: 18px;"> | ||
+ | <td style="height: 18px; width: 110px;">BBa_K2217013</td> | ||
+ | <td style="height: 18px; width: 547px;">CAG Promoter</td> | ||
+ | <td style="height: 18px; width: 54px;">590</td> | ||
+ | <td style="height: 18px; width: 39px;"><a href="http://parts.igem.org/Part:BBa_K2217013">HERE</a></td> | ||
+ | </tr> | ||
+ | <tr style="height: 18px;"> | ||
+ | <td style="height: 18px; width: 110px;">BBa_K2217023</td> | ||
+ | <td style="height: 18px; width: 547px;">YFP for ceRNA circuit characterization</td> | ||
+ | <td style="height: 18px; width: 54px;">1015</td> | ||
+ | <td style="height: 18px; width: 39px;"><a href="http://parts.igem.org/Part:BBa_K2217023">HERE</a></td> | ||
+ | </tr> | ||
+ | <tr style="height: 18px;"> | ||
+ | <td style="height: 18px; width: 110px;">BBa_K2217018</td> | ||
+ | <td style="height: 18px; width: 547px;">gRNA for Cas9 Knock-in</td> | ||
+ | <td style="height: 18px; width: 54px;">483</td> | ||
+ | <td style="height: 18px; width: 39px;"><a href="http://parts.igem.org/Part:BBa_K2217018">HERE</a></td> | ||
+ | </tr> | ||
+ | <tr style="height: 28px;"> | ||
+ | <td style="height: 28px; width: 110px;">BBa_K2217029</td> | ||
+ | <td style="height: 28px; width: 547px;">CMV Enhancer + CMV Promoter</td> | ||
+ | <td style="height: 28px; width: 54px;">516</td> | ||
+ | <td style="height: 28px; width: 39px;"><a href="http://parts.igem.org/Part:BBa_K2217029">HERE</a></td> | ||
+ | </tr> | ||
+ | </tbody> | ||
+ | </table> | ||
+ | <p style="text-align: center;"><br /><br /><br /></p> | ||
+ | </body> | ||
</html> | </html> | ||
+ | {{Template:AFCM-Egypt/footer}} |
Revision as of 16:18, 26 October 2017
Part Name | Description | Length | Link |
---|---|---|---|
BBa_K2217019 | Cas9 Knock-in | 6035 | HERE |
BBa_K2217020 | BBa_K2217001 ceRNA hsa-circ-0000064 | 867 | HERE |
BBa_K2217022 | laci + miRNA-1825 binding site on hsa-circ-0000064 Production | 1732d | HERE |
Fragments
These composite parts were synthesised as separate gene fragments then assembled to form BBa_K2217025 and BBa_K2217026 representing ceRNA and CRISPR circuits respectively.
Part Name | Description | Length | Link |
---|---|---|---|
BBa_K2217024 | CMV Enhancer + CMV Promoter + T7 | 542 | HERE |
BBa_K2217027 | laci + miRNA-1825 binding site synthesized fragment | 1210 | HERE |
BBa_K2217028 | BBa_K2217001 ceRNA hsa-circ-0000064 + sv_poly(A) Synthesized Fragment | 269 | HERE |
BBa_K2217013 | CAG Promoter | 590 | HERE |
BBa_K2217023 | YFP for ceRNA circuit characterization | 1015 | HERE |
BBa_K2217018 | gRNA for Cas9 Knock-in | 483 | HERE |
BBa_K2217029 | CMV Enhancer + CMV Promoter | 516 | HERE |