Difference between revisions of "Team:AFCM-Egypt/Part Collection"

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Revision as of 17:35, 26 October 2017

A F C M

A F C M
Parts

We submitted the basic parts constituting our gene fragments, then
we designed the composite parts constituting these synthesised fragments
and finally we assembled these composite parts into our two main circuits
designed in order to compare their function at regulating non-coding RNAs.


Basic Parts

Part Name Type Description Length Link
BBa_K2217000 Basic CMV Enhancer 304 HERE
BBa_K2217001 Basic Circular RNA hsa-circ-000064 139 HERE
BBa_K2217002 Basic U6 Promoter for Cas9 gRNA 204 HERE
BBa_K2217003 Basic gRNA for Cas9 Knock in 20 HERE
BBa_K2217004 Basic gRNA scaffold for Cas9 76 HERE
BBa_K2217005 Basic SV40 Terminator 122 HERE
BBa_K2217006 Basic CMV Promoter 204 HERE
BBa_K2217007 Basic T7 Promoter 19 HERE
BBa_K2217008 Basic CYC1 Terminator 250 HERE
BBa_K2217009 Basic HDR Template of BBa_K2217001 139 HERE
BBa_K2217010 Basic Chicken B-actin Promoter 278 HERE
BBa_K2217012 Basic laci 1080 HERE
BBa_K2217014 Basic YFP 720 HERE
BBa_K2217015 Basic bGH poly(A) signal 225 HERE
BBa_K2217016 Basic lac_operator 17 HERE
BBa_K2217017 Basic lac_promoter 31 HERE
BBa_K2217021 Basic miRNA-1825 binding site on hsa-circ-00064 20 HERE







Composite Parts

Part Name Description Length Link
BBa_K2217019 Cas9 Knock-in 6035 HERE
BBa_K2217020 BBa_K2217001 ceRNA hsa-circ-0000064 867 HERE
BBa_K2217022 laci + miRNA-1825 binding site on hsa-circ-0000064 Production 1732d HERE





Composite Parts representing Synthesized
Fragments

These composite parts were synthesised as separate gene fragments then assembled to form BBa_K2217025 and BBa_K2217026 representing ceRNA and CRISPR circuits respectively.

Part Name Description Length Link
BBa_K2217024 CMV Enhancer + CMV Promoter + T7 542 HERE
BBa_K2217027 laci + miRNA-1825 binding site synthesized fragment 1210 HERE
BBa_K2217028 BBa_K2217001 ceRNA hsa-circ-0000064 + sv_poly(A) Synthesized Fragment 269 HERE
BBa_K2217013 CAG Promoter 590 HERE
BBa_K2217023 YFP for ceRNA circuit characterization 1015 HERE
BBa_K2217018 gRNA for Cas9 Knock-in 483 HERE
BBa_K2217029 CMV Enhancer + CMV Promoter 516 HERE