Difference between revisions of "Team:AFCM-Egypt/Improve"

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<h3>★ ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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<p style="text-align: center;"><span style="font-size: 18.0pt; line-height: 107%;"><br /><span style="color: #ffffff;">Improved parts</span></span></p>
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<p><span style="font-size: 18pt; line-height: 107%; color: #ffffff;">&nbsp;</span></p>
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<li><span style="color: #ffffff;"><span style="font-size: 14.0pt; line-height: 107%; font-family: 'Arial','sans-serif';">BBa_K2217013:</span> <span style="font-size: 14pt; line-height: 107%; font-family: Arial, sans-serif; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">We tried to improve CAG promoter at&nbsp;</span><a style="color: #ffffff;" href="http://parts.igem.org/Part:BBa_K1179069"><span style="font-size: 14pt; line-height: 107%; font-family: Arial, sans-serif;">BBa_K1179069</span></a><span style="font-size: 14pt; line-height: 107%; font-family: Arial, sans-serif; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">&nbsp;</span><span style="font-size: 14pt; line-height: 107%; font-family: Arial, sans-serif; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">by making it&nbsp;</span><a style="color: #ffffff;" href="http://tools.ietf.org/html/rfc10"><span style="font-size: 14pt; line-height: 107%; font-family: Arial, sans-serif;">RFC 10</span></a><span style="font-size: 14pt; line-height: 107%; font-family: Arial, sans-serif; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">&nbsp;</span><span style="font-size: 14pt; line-height: 107%; font-family: Arial, sans-serif; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;"> CMV enhancer + chicken beta-actin promoter, the part was improved by sequence adjustment to gene block synthesis requirements The sequence GGC was repeated 6 consecutive times starting at base 538 and a homopolymer run of 16 G bases is present starting at base 413 was reduced less than 9.<br /><br /><br /></span></span></li>
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<li><span style="color: #ffffff;"><span style="font-size: 14.0pt; line-height: 107%; font-family: 'Arial','sans-serif';">BBa_K2217024: We </span><span style="font-size: 14pt; line-height: 107%; font-family: Arial, sans-serif; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">improved this part</span><a style="color: #ffffff;" href="http://parts.igem.org/Part:BBa_K1119006"><span style="font-size: 14pt; line-height: 107%; font-family: Arial, sans-serif;">BBa_K1119006</span></a><span style="font-size: 14pt; line-height: 107%; font-family: Arial, sans-serif; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">&nbsp;</span><span style="font-size: 14pt; line-height: 107%; font-family: Arial, sans-serif; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">by Fusing CMV enhancer to CMV promoter in one composite part to enhance its transcription activity</span><a style="color: #ffffff;" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2217024"><span style="font-size: 14pt; line-height: 107%; font-family: Arial, sans-serif;">BBa_K2217024</span></a><span style="font-size: 14pt; line-height: 107%; font-family: Arial, sans-serif; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">. Part characterization and usage can be found at&nbsp;</span><a style="color: #ffffff;" href="http://parts.igem.org/Part:BBa_K2217024:Experience"><span style="font-size: 14pt; line-height: 107%; font-family: Arial, sans-serif;">Part Experience</span></a><span style="font-size: 14.0pt; line-height: 107%; font-family: 'Arial','sans-serif';">.<br /><br /></span></span></li>
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<li><span style="color: #ffffff;"><span style="font-size: 14.0pt; line-height: 107%; font-family: 'Arial','sans-serif';">&nbsp;</span><span style="font-size: 14.0pt; font-family: 'Arial','sans-serif';">BBa_K2217027: </span><span style="font-size: 14pt; font-family: Arial, sans-serif;">tried to improve laci function at&nbsp;</span><a style="color: #ffffff;" href="http://parts.igem.org/Part:BBa_K1179062"><span style="font-size: 14pt; font-family: Arial, sans-serif;">BBa_K1179062</span></a><span style="font-size: 14pt; font-family: Arial, sans-serif;">&nbsp;</span><span style="font-size: 14pt; font-family: Arial, sans-serif;">regarding inhibition of non-coding RNAs by fusing laci the binding site of the miRNA mir-1825 to hsa_circ_0000064 in one composite part to help controlling circular RNA hsa_circ_0000064 transcription at BBa_K2217001 in response to miRNA-1825 binding to this sensor binding site. Part characterization and usage can be found at&nbsp;</span><a style="color: #ffffff;" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2217027"><span style="font-size: 14pt; font-family: Arial, sans-serif;">BBa_K2217027_Experience</span></a></span><span style="font-size: 14.0pt; font-family: 'Arial','sans-serif'; color: #282828;"><span style="color: #ffffff;">.</span><br /><br /><span style="color: #ffffff;">&nbsp;Laci registered at&nbsp;<a style="color: #ffffff;" href="http://parts.igem.org/Part:BBa_K2217012"><span style="font-size: 14pt; font-family: Arial, sans-serif;">(BBa_K2217012)</span></a>, was fused to the miRNA binding site of the miRNA mir-1825 as determined computationally using circinteractome database, where we hypothesized its function as a sensor for miRNA binding.</span><br /><br /><br /><br /></span></li>
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<h1>Improve</h1>
 
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<p>For teams seeking to improve upon a previous part or project, you should document all of your work on this page. Please remember to include all part measurement and characterization data on the part page on the Regisrty. Please include a link to your improved part on this page.</p>
 
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<h3>Gold Medal Criterion #2</h3>
 
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<p><b>Standard Tracks:</b> Improve the function of an existing BioBrick Part. The original part must NOT be from your 2017 part number range. If you change the original part sequence, you must submit a new part. In addition, both the new and original part pages must reference each other. This working part must be different from the part documented in bronze #4 and silver #1.
 
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<b>Special Tracks:</b> Improve the function of an existing iGEM project (that your current team did not originally create) and display your achievement on your wiki.</p>
 
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Revision as of 17:53, 26 October 2017

A F C M

A F C M


Improved parts

 

  1. BBa_K2217013: We tried to improve CAG promoter at BBa_K1179069 by making it RFC 10  CMV enhancer + chicken beta-actin promoter, the part was improved by sequence adjustment to gene block synthesis requirements The sequence GGC was repeated 6 consecutive times starting at base 538 and a homopolymer run of 16 G bases is present starting at base 413 was reduced less than 9.


  2. BBa_K2217024: We improved this partBBa_K1119006 by Fusing CMV enhancer to CMV promoter in one composite part to enhance its transcription activityBBa_K2217024. Part characterization and usage can be found at Part Experience.

  3.  BBa_K2217027: tried to improve laci function at BBa_K1179062 regarding inhibition of non-coding RNAs by fusing laci the binding site of the miRNA mir-1825 to hsa_circ_0000064 in one composite part to help controlling circular RNA hsa_circ_0000064 transcription at BBa_K2217001 in response to miRNA-1825 binding to this sensor binding site. Part characterization and usage can be found at BBa_K2217027_Experience.

     Laci registered at (BBa_K2217012), was fused to the miRNA binding site of the miRNA mir-1825 as determined computationally using circinteractome database, where we hypothesized its function as a sensor for miRNA binding.