Difference between revisions of "Team:AFCM-Egypt/Improve"
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| − | <li><span style="color: #ffffff;"><span style="font-size: 14.0pt; line-height: 107%; font-family: 'Arial','sans-serif';">BBa_K2217013:</span> <span style="font-size: 14pt; line-height: 107%; font-family: Arial, sans-serif; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">We tried to improve CAG promoter at </span>< | + | <li><span style="color: #ffffff;"><span style="font-size: 14.0pt; line-height: 107%; font-family: 'Arial','sans-serif';">BBa_K2217013:</span> <span style="font-size: 14pt; line-height: 107%; font-family: Arial, sans-serif; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">We tried to improve CAG promoter at </span><span style="text-decoration: underline;"><a href="http://parts.igem.org/Part:BBa_K1179069"><span style="font-size: 14pt; line-height: 107%; font-family: Arial, sans-serif; color: #ffff99; text-decoration: underline;">BBa_K1179069</span><span style="font-size: 14pt; line-height: 107%; font-family: Arial, sans-serif; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;"> </span></a></span><span style="font-size: 14pt; line-height: 107%; font-family: Arial, sans-serif; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">by making it </span><span style="color: #ffff99;"><a style="color: #ffff99;" href="http://tools.ietf.org/html/rfc10"><span style="font-size: 14pt; line-height: 107%; font-family: Arial, sans-serif;">RFC 10</span></a><span style="font-size: 14pt; line-height: 107%; font-family: Arial, sans-serif; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;"> </span></span><span style="font-size: 14pt; line-height: 107%; font-family: Arial, sans-serif; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;"> CMV enhancer + chicken beta-actin promoter, the part was improved by sequence adjustment to gene block synthesis requirements The sequence GGC was repeated 6 consecutive times starting at base 538 and a homopolymer run of 16 G bases is present starting at base 413 was reduced less than 9.<br /><br /><br /></span></span></li> |
| − | <li><span style="color: #ffffff;"><span style="font-size: 14.0pt; line-height: 107%; font-family: 'Arial','sans-serif';">BBa_K2217024: We </span><span style="font-size: 14pt; line-height: 107%; font-family: Arial, sans-serif; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">improved this part</span><a style="color: # | + | <li><span style="color: #ffffff;"><span style="font-size: 14.0pt; line-height: 107%; font-family: 'Arial','sans-serif';">BBa_K2217024: We </span><span style="font-size: 14pt; line-height: 107%; font-family: Arial, sans-serif; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">improved this part </span><span style="color: #ffff99;"><a style="color: #ffff99;" href="http://parts.igem.org/Part:BBa_K1119006"><span style="font-size: 14pt; line-height: 107%; font-family: Arial, sans-serif;">BBa_K1119006</span></a></span><span style="font-size: 14pt; line-height: 107%; font-family: Arial, sans-serif; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;"> </span><span style="font-size: 14pt; line-height: 107%; font-family: Arial, sans-serif; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">by Fusing CMV enhancer to CMV promoter in one composite part to enhance its transcription activity </span><span style="color: #ffff99;"><a style="color: #ffff99;" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2217024"><span style="font-size: 14pt; line-height: 107%; font-family: Arial, sans-serif;">BBa_K2217024</span></a></span><span style="font-size: 14pt; line-height: 107%; font-family: Arial, sans-serif; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">. Part characterization and usage can be found at </span><span style="color: #ffff99;"><a style="color: #ffff99;" href="http://parts.igem.org/Part:BBa_K2217024:Experience"><span style="font-size: 14pt; line-height: 107%; font-family: Arial, sans-serif;">Part Experience</span></a></span><span style="font-size: 14.0pt; line-height: 107%; font-family: 'Arial','sans-serif';"><a href="http://parts.igem.org/Part:BBa_K2217024:Experience"><span style="color: #ffff99;">.</span></a><br /><br /></span></span></li> |
| − | <li><span style="color: #ffffff;"><span style="font-size: 14.0pt; line-height: 107%; font-family: 'Arial','sans-serif';"> </span><span style="font-size: 14.0pt; font-family: 'Arial','sans-serif';">BBa_K2217027: </span><span style="font-size: 14pt; font-family: Arial, sans-serif;">tried to improve laci function at </span><a style="color: # | + | <li><span style="color: #ffffff;"><span style="font-size: 14.0pt; line-height: 107%; font-family: 'Arial','sans-serif';"> </span><span style="font-size: 14.0pt; font-family: 'Arial','sans-serif';">BBa_K2217027: </span><span style="font-size: 14pt; font-family: Arial, sans-serif;">tried to improve laci function at </span><span style="color: #ffff99;"><a style="color: #ffff99;" href="http://parts.igem.org/Part:BBa_K1179062"><span style="font-size: 14pt; font-family: Arial, sans-serif;">BBa_K1179062</span></a></span><span style="font-size: 14pt; font-family: Arial, sans-serif;"> </span><span style="font-size: 14pt; font-family: Arial, sans-serif;">regarding inhibition of non-coding RNAs by fusing laci the binding site of the miRNA mir-1825 to hsa_circ_0000064 in one composite part to help controlling circular RNA hsa_circ_0000064 transcription at BBa_K2217001 in response to miRNA-1825 binding to this sensor binding site. Part characterization and usage can be found at </span><span style="color: #ffff99;"><a style="color: #ffff99;" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2217027"><span style="font-size: 14pt; font-family: Arial, sans-serif;">BBa_K2217027_Experience</span></a></span></span><span style="font-size: 14.0pt; font-family: 'Arial','sans-serif'; color: #282828;"><span style="color: #ffff99;">.</span><br /><br /><span style="color: #ffffff;"> Laci registered at <a style="color: #ffffff;" href="http://parts.igem.org/Part:BBa_K2217012"><span style="font-size: 14pt; font-family: Arial, sans-serif;">(<span style="color: #ffff99;">BBa_K2217012</span>)</span></a>, was fused to the miRNA binding site of the miRNA mir-1825 as determined computationally using circinteractome database, where we hypothesized its function as a sensor for miRNA binding.</span><br /><br /><br /><br /></span></li> |
</ol> | </ol> | ||
</div> | </div> | ||
Revision as of 18:00, 26 October 2017
Improved parts
- BBa_K2217013: We tried to improve CAG promoter at BBa_K1179069 by making it RFC 10 CMV enhancer + chicken beta-actin promoter, the part was improved by sequence adjustment to gene block synthesis requirements The sequence GGC was repeated 6 consecutive times starting at base 538 and a homopolymer run of 16 G bases is present starting at base 413 was reduced less than 9.
- BBa_K2217024: We improved this part BBa_K1119006 by Fusing CMV enhancer to CMV promoter in one composite part to enhance its transcription activity BBa_K2217024. Part characterization and usage can be found at Part Experience.
- BBa_K2217027: tried to improve laci function at BBa_K1179062 regarding inhibition of non-coding RNAs by fusing laci the binding site of the miRNA mir-1825 to hsa_circ_0000064 in one composite part to help controlling circular RNA hsa_circ_0000064 transcription at BBa_K2217001 in response to miRNA-1825 binding to this sensor binding site. Part characterization and usage can be found at BBa_K2217027_Experience.
Laci registered at (BBa_K2217012), was fused to the miRNA binding site of the miRNA mir-1825 as determined computationally using circinteractome database, where we hypothesized its function as a sensor for miRNA binding.
