Difference between revisions of "Team:Lethbridge/Improve"

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<div><img src="https://static.igem.org/mediawiki/2017/9/95/Banner_PAimproved.png"  class="center fit"></div>
 
<div><img src="https://static.igem.org/mediawiki/2017/9/95/Banner_PAimproved.png"  class="center fit"></div>
 
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                <div class="content_box">
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<div class="segmentDiv">
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                    <h1>Improved Parts</h1>
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            <h2 class="segmentHeader">Characterization</h2>
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                    <h2>BBa_K2443037 - T7 RNA Polymerase</h2>
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<h2 align= "left"> Methionine Synthetase (MetRS)</h2>
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<p><b>Original part:</b> BBa_I2032</p>  
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<p class="pageText"> <b> Original part:</b> BBa_K567015
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<p><b>Submitted by:</b> MIT 2006</p>
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</p>
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<p><b>designed by:</b> Bartholomew Canton</p>
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<p class="pageText"> <b> Submitted by:</b> SJTU-BioX-Shanghai 2011
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<p><b>Rational behind improvements:</b></p>
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</p>
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<p>BBa_I2032 encodes exclusively for the coding region of T7 RNA polymerase. In order to improve it and incorporate it into our Next<i>Vivo</i> system we have Codon optimized for use in <i>Escherichia coli</i> and attached a N-terminal 6 X His tag with a serine glycine linker for easy purification. We have also optimized our T7 RNA polymerase to be overexpressed in BL21DE3 gold cells by putting it under the control of a T7 promoter (BBa_I719005), RBS (BBa_B0034) and double terminator (BBa_B0015)</P>  
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<p class="pageText"> <b> Designed by:</b> You Wang
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</p>
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<p class="pageText"> <b>Rational behind improvements:</b>
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</p>
 +
 
 +
<br>
 +
 
 +
<h2 align= "left"> T7 RNA Polymerase</h2>
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<p class="pageText"> <b> Original part:</b> BBa_I2032
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</p>
 +
<p class="pageText"> <b> Submitted by:</b> MIT
 +
</p>
 +
<p class="pageText"> <b> Designed by:</b> Bartholomew Canton
 +
</p>
 +
<p class="pageText"> <b> Rational behind improvements:</b> BBa_I2032 encodes exclusively for the coding region of T7 RNA polymerase. In order to improve it and incorporate it into our Next<i>Vivo</i> system we have Codon optimized for use in <i>Escherichia coli</i> and attached a N-terminal 6 X His tag with a serine glycine linker for easy purification. We have also optimized our T7 RNA polymerase to be overexpressed in BL21DE3 gold cells by putting it under the control of a T7 promoter (BBa_I719005), RBS (BBa_B0034) and double terminator (BBa_B0015)
 +
</p>
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 +
<br>
 +
 
 +
<h2 align= "left"> Elongation Factor Thermo Stable (EF-Ts)</h2>
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<p class="pageText"> <b> Original part:</b> BBa_K1906000
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</p>
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<p class="pageText"> <b> Submitted by:</b> XJTLU-China 2016
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</p>
 +
<p class="pageText"> <b> Designed by:</b> Wenbo Xu
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</p>
 +
<p class="pageText"> <b> Rational behind improvements:</b>
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</p>
  
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<h2>BBa_K2443028 - EF-Ts </h2>
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<br>
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<p><b>Original part:</b>BBa_K1906000</p>
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<p><b>Submitted by:</b> XJTLU-China 2016</p>
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−
<p><b>designed by:</b> Wenbo Xu</p>
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−
<p><b>Rational behind improvements:</b></p>
+
−
<p>BBa_I2032 encodes exclusively for the coding region of T7 RNA polymerase. In order to improve it and incorporate it into our Next<i>Vivo</i> system we have Codon optimized for use in <i>Escherichia coli</i> and attached a N-terminal 6 X His tag with a serine glycine linker for easy purification. We have also optimized our T7 RNA polymerase to be overexpressed in BL21DE3 gold cells by putting it under the control of a T7 promoter (BBa_I719005), RBS (BBa_B0034) and double terminator (BBa_B0015)</P> 
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<h2>BBa_K2443027 - EF-Tu</h2>
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<h2 align= "left"> Elongation Factor Thermo Unstable (EF-Tu)</h2>
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<p><b>Original part:</b> BBa_K1906004</p>  
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<p class="pageText"> <b> Original part:</b> BBa_K1906004
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<p><b>Submitted by:</b> XJTLU-China 2016</p>
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</p>
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<p><b>designed by:</b>Yuwei Han </p>
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<p class="pageText"> <b> Submitted by:</b> XJTLU-China 2016
−
<p><b>Rational behind improvements:</b></p>
+
</p>
−
<p>BBa_I2032 encodes exclusively for the coding region of T7 RNA polymerase. In order to improve it and incorporate it into our Next<i>Vivo</i> system we have Codon optimized for use in <i>Escherichia coli</i> and attached a N-terminal 6 X His tag with a serine glycine linker for easy purification. We have also optimized our T7 RNA polymerase to be overexpressed in BL21DE3 gold cells by putting it under the control of a T7 promoter (BBa_I719005), RBS (BBa_B0034) and double terminator (BBa_B0015)</P> 
+
<p class="pageText"> <b> Designed by:</b>Yuwei Han
 +
</p>
 +
<p class="pageText"> <b> Rational behind improvements:</b>
 +
</p>
  
−
<h2>BBa_K2443013 - Met-RS</h2>
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<br>
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<p><b>Original part:</b>BBa_K567015</p>
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−
<p><b>Submitted by:</b> SJTU-BioX-Shanghai 2011</p>
+
−
<p><b>designed by:</b>You Wang</p>
+
−
<p><b>Rational behind improvements:</b></p>
+
−
<p>BBa_I2032 encodes exclusively for the coding region of T7 RNA polymerase. In order to improve it and incorporate it into our Next<i>Vivo</i> system we have Codon optimized for use in <i>Escherichia coli</i> and attached a N-terminal 6 X His tag with a serine glycine linker for easy purification. We have also optimized our T7 RNA polymerase to be overexpressed in BL21DE3 gold cells by putting it under the control of a T7 promoter (BBa_I719005), RBS (BBa_B0034) and double terminator (BBa_B0015)</P> 
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Revision as of 22:01, 27 October 2017

Characterization

Methionine Synthetase (MetRS)

Original part: BBa_K567015

Submitted by: SJTU-BioX-Shanghai 2011

Designed by: You Wang

Rational behind improvements:


T7 RNA Polymerase

Original part: BBa_I2032

Submitted by: MIT

Designed by: Bartholomew Canton

Rational behind improvements: BBa_I2032 encodes exclusively for the coding region of T7 RNA polymerase. In order to improve it and incorporate it into our NextVivo system we have Codon optimized for use in Escherichia coli and attached a N-terminal 6 X His tag with a serine glycine linker for easy purification. We have also optimized our T7 RNA polymerase to be overexpressed in BL21DE3 gold cells by putting it under the control of a T7 promoter (BBa_I719005), RBS (BBa_B0034) and double terminator (BBa_B0015)


Elongation Factor Thermo Stable (EF-Ts)

Original part: BBa_K1906000

Submitted by: XJTLU-China 2016

Designed by: Wenbo Xu

Rational behind improvements:


Elongation Factor Thermo Unstable (EF-Tu)

Original part: BBa_K1906004

Submitted by: XJTLU-China 2016

Designed by:Yuwei Han

Rational behind improvements: