Difference between revisions of "Team:Lethbridge/Improve"

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<p class="pageText"> <b> Designed by:</b> Bartholomew Canton
 
<p class="pageText"> <b> Designed by:</b> Bartholomew Canton
 
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<p class="pageText"> <b> Rational behind improvements:</b> BBa_I2032 encodes exclusively for the coding region of T7 RNA polymerase. In order to improve it and incorporate it into our Next<i>Vivo</i> system we have Codon optimized for use in <i>Escherichia coli</i> and attached a N-terminal 6 X His tag with a serine glycine linker for easy purification. We have also optimized our T7 RNA polymerase to be overexpressed in BL21DE3 gold cells by putting it under the control of a T7 promoter (BBa_I719005), RBS (BBa_B0034) and double terminator (BBa_B0015)
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<p class="pageText"> <b> Rational behind improvements:</b> BBa_I2032 encodes exclusively for the coding region of T7 RNA polymerase. In order to improve it and incorporate it into our N<i>ex</i>t <i>vivo</i> system we have Codon optimized for use in <i>Escherichia coli</i> and attached a N-terminal 6 X His tag with a serine glycine linker for easy purification. We have also optimized our T7 RNA polymerase to be overexpressed in BL21DE3 gold cells by putting it under the control of a T7 promoter (BBa_I719005), RBS (BBa_B0034) and double terminator (BBa_B0015)
 
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Revision as of 22:10, 27 October 2017

Characterization

Methionine Synthetase (MetRS)

Original part: BBa_K567015

Submitted by: SJTU-BioX-Shanghai 2011

Designed by: You Wang

Rational behind improvements:


T7 RNA Polymerase (T7 RNAP)

Original part: BBa_I2032

Submitted by: MIT

Designed by: Bartholomew Canton

Rational behind improvements: BBa_I2032 encodes exclusively for the coding region of T7 RNA polymerase. In order to improve it and incorporate it into our Next vivo system we have Codon optimized for use in Escherichia coli and attached a N-terminal 6 X His tag with a serine glycine linker for easy purification. We have also optimized our T7 RNA polymerase to be overexpressed in BL21DE3 gold cells by putting it under the control of a T7 promoter (BBa_I719005), RBS (BBa_B0034) and double terminator (BBa_B0015)


Elongation Factor Thermo Stable (EF-Ts)

Original part: BBa_K1906000

Submitted by: XJTLU-China 2016

Designed by: Wenbo Xu

Rational behind improvements:


Elongation Factor Thermo Unstable (EF-Tu)

Original part: BBa_K1906004

Submitted by: XJTLU-China 2016

Designed by:Yuwei Han

Rational behind improvements: