Collaboration with SIAT-SCIE

In the collaboration with SIAT-SCIE, we held a workshop to communicate our projects. We also help them with the molecular experiment.

After several online contacts, we invited SIAT-SCIE to our lab and held the first workshop in SUSTech. At first, half part of the workshop were introducing our own project, and we prepared a brief speech concerning our design and some background knowledge like microinjection and microfluidics. After our presentation, SIAT-SCIE also proposed their idea about the project and showed some supporting papers.

What’s more, beyond only talking about project, we also deeply exchanged the ideas about how to manage such a project on our own. And we made an agreement that working in smaller groups and having group meeting is a good way to coordinate work progress and form a good working pattern.

Fig 14: SIAT-SCIE project introduction

Fig 14.5: Workshop with SIAT-SCIE

Besides exchanging ideas, collaboration was also meaningful by giving help to other iGEM teams, who faced difficulties. For example, we taught them how to construct plasmids for subcloning and helped SIAT-SCIE construct their core plasmid in their project.

Fig 15: SIAT-SCIE core plasmid

We also participated in South China iGEMer Meeting up held by SIAT-SCIE. The aim of this meeting up was iGEM team communication between high schools. We also attended the meeting up and shared our ideas with other high school teams.

Fig 16: South China iGEMer Meeting up

STAT-SCIE Wiki

Collaboration with Shenzhen_SFLS

In the early July, Shenzhen_SFLS contacted us for some help. They wanted to get instructions on modeling and also faced problems of protein engineering project.

In their protein-engineering project, they found their interested protein was coated in inclusion body by BL21. So they cannot extract enough proteins out of engineering bacteria. So we contacted Prof. Hongmin Zhang in SUSTech and held a seminar with them to discuss how to deal with inclusion body. Prof. Zhang introduced the protocol of inclusion body washing, dissolution and renaturation, which helped them avoid expensive protein synthesis.

Fig 17: Seminar with Prof. Zhang and Shenzhen_SFLS

We also held a workshop to exchange project ideas. And our teammate Yuejian Mo was invited to give some instructions for their model.

Shenzhen_SFL Wiki

Fig 18: Introduce C.elegans to Shenzhen_SFLS