Difference between revisions of "Team:Lethbridge/Experiments"

Enzymes	Buffers/Reagents	Other Products
Shrimp Alkaline Phosphatase	Affinity Chromatography Buffers	Ni²+ Affinity Resin
DNA Blunting Enzyme	IPTG	Prefix Forward: 5’GAATTCGCGGCCGCTTCTAG3’
Phusion Polymerase Master Mix	Kanomycin/Chloramphenicol/Ampicillin	Prefix Reverse: 5’CTGCAGCGGCCGCTACTAGTA3’
PFU Polymerase	Dimethyl Sulfur Dioxide	Prefix Forward +6:5’GGCTCAGAATTCGCGGCCGCTTCTAGAG3’
T7 RNA Polymerase	Competent Cell Buffers	Suffix Reverse +6: 5’CTGGACCTGCAGCGGCCGCTACTAGTA3’
T7 DNA Polymerase	T7 RNA Polymerase Purification Buffers	Dialyzation Membrane
PURExpressKit (New England Biolabs)	Phenol
XbaI (NEB)	Chloroform
SpeI (NEB)	10x PFU Buffer
PstI (NEB)	NTP
EcoRI (NEB)	GMP
Dream Taq DNA Polymerase	10x Taq Buffer
Taq DNA Polymerase	Ligase Buffer
RNAse A	SDS-PAGE Reagents
TraB	Urea PAGE reagents
iPPase	Agarose
RNAse Inhibitor	Agar
DNAseI	Tryptone
	Yeast Extract
	TAKMz
	BioBasic Plasmid DNA miniprep Kit
	Imidazole

@@ Line 78: / Line 78: @@
 <p><a href="https://static.igem.org/mediawiki/2017/5/51/In_vitro_transcription.pdf">In Vitro Transcription</a></p>
-<p><a href="protocol">Ligations</a></p>
+<p><a href="https://static.igem.org/mediawiki/2017/6/6c/Ligation_protocol.pdf">Ligations</a></p>
-<p><a href="protocol">Phenol/Chloroform Extraction and Ethanol Precipitation</a></p>
+<p><a href="https://static.igem.org/mediawiki/2017/d/df/PhenolChloroform_extraction_%26_ethanol_percipitation.pdf">Phenol/Chloroform Extraction and Ethanol Precipitation</a></p>
-<p><a href="protocol">pJET Cloning</a></p>
+<p><a href="https://static.igem.org/mediawiki/2017/e/eb/PJET_Cloning.pdf">pJET Cloning</a></p>
-<p><a href="protocol">Plasmid Miniprep</a></p>
+<p><a href="https://static.igem.org/mediawiki/2017/0/08/Plasmid_Miniprep.pdf">Plasmid Miniprep</a></p>
-<p><a href="protocol">Preparation of Chemical Competent Cells</a></p>
+<p><a href="https://static.igem.org/mediawiki/2017/7/78/Preparation_of_Chemical_Competent_Cells.pdf">Preparation of Chemical Competent Cells</a></p>
-<p><a href="protocol">Protein Overexpression</a></p>
+<p><a href="https://static.igem.org/mediawiki/2017/2/2c/Protein_Overexpression_.pdf">Protein Overexpression</a></p>
-<p><a href="protocol">PURExpress NEB Kit</a></p>
+<p><a href="https://static.igem.org/mediawiki/2017/5/5f/PURExpress.pdf">PURExpress NEB Kit</a></p>
-<p><a href="protocol">Purification of Poly-Histidine Tagged Proteins</a></p>
+<p><a href="https://static.igem.org/mediawiki/2017/f/fc/Purification_of_Poly-Histidine_Tagged_Proteins.pdf">Purification of Poly-Histidine Tagged Proteins</a></p>
-<p><a href="protocol">Restriction Enzyme Digest and Dephosphorylation</a></p>
+<p><a href="https://static.igem.org/mediawiki/2017/1/10/Restriction_Enzyme_Digest_and_Dephosphorylation.pdf">Restriction Enzyme Digest and Dephosphorylation</a></p>
-<p><a href="protocol">T7- RNA Polymerase Purification</a></p>
+<p><a href="https://static.igem.org/mediawiki/2017/2/2a/T7-RNA_Polymerase_Purification.pdf">T7- RNA Polymerase Purification</a></p>
-<p><a href="protocol">Transformation</a></p>
+<p><a href="https://static.igem.org/mediawiki/2017/a/a6/Transformation.pdf">Transformation</a></p>
 <br><br><br><br>