Difference between revisions of "Team:Lethbridge/InterLab"
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| + | <p class="pageText"> The ability for parts to provide repeatable outputs is key to engineering applications, including synthetic biology. The purpose of the 2017 interlab study was to address how reproducible and at what levels protein expression could be driven by standardized promoters and ribosomal binding sites. Constructs were provided by the iGEM measurement committee that utilized green fluorescent protein as a reporter. This allowed interlab study contributors to easily follow protein expression driven by the standardized promoters and ribosomal binding sites. | ||
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| + | In order to better compare expression levels between research groups, the 2017 interlab study incorporated calibration protocols to allow for reporting of fluorescence levels in absolute levels. Specifically, fluorescence levels from biological samples were calibrated against fluorescence from a fluorescein standard curve, allowing for fluorescence results to be reported in terms of the amount of fluorescence produced by a certain concentration of fluorescein. | ||
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Revision as of 16:41, 28 October 2017
Overview
The ability for parts to provide repeatable outputs is key to engineering applications, including synthetic biology. The purpose of the 2017 interlab study was to address how reproducible and at what levels protein expression could be driven by standardized promoters and ribosomal binding sites. Constructs were provided by the iGEM measurement committee that utilized green fluorescent protein as a reporter. This allowed interlab study contributors to easily follow protein expression driven by the standardized promoters and ribosomal binding sites.
In order to better compare expression levels between research groups, the 2017 interlab study incorporated calibration protocols to allow for reporting of fluorescence levels in absolute levels. Specifically, fluorescence levels from biological samples were calibrated against fluorescence from a fluorescein standard curve, allowing for fluorescence results to be reported in terms of the amount of fluorescence produced by a certain concentration of fluorescein.
