ShirOvadia (Talk | contribs) |
|||
Line 143: | Line 143: | ||
<td>Device</td> | <td>Device</td> | ||
<td>TRE-GFP-hGH</td> | <td>TRE-GFP-hGH</td> | ||
− | <td>Noa Eden, Dana Kadosh, Maya Engal</td> | + | <td>Noa Eden, Dana Kadosh, Maya Engal, Shir Ovadia</td> |
<td>1532</td> | <td>1532</td> | ||
</tr> | </tr> | ||
Line 184: | Line 184: | ||
<td>Device</td> | <td>Device</td> | ||
<td>CMV-tTA-hGH</td> | <td>CMV-tTA-hGH</td> | ||
− | <td>Noa Eden, Dana Kadosh, Maya Engal</td> | + | <td>Noa Eden, Dana Kadosh, Maya Engal, Shir Ovadia</td> |
<td>1860</td> | <td>1860</td> | ||
</tr> | </tr> |
Revision as of 00:25, 29 October 2017
Composite Parts
![](https://static.igem.org/mediawiki/2017/f/f9/T--TECHNION-ISRAEL--co-assay.png)
We have designed several complete devices in order to display multiple proteins on the membrane of mammalian cells using a single system. Our favorite composite part is TRE-Tri_display-hGH (BBa_K2520007). This composite part consists of the TRE promoter, our novel Tri-Display system and the hGH terminator. The Tri-Display is a modular display vector capable of displaying up to three different proteins on the membrane of mammalian cells in equimolar ratios. The Tri-Display contains three display segments connected by P2A (cleavage sites). Each display component consists of an Igk leader (signal peptide), a protein tag (for easy detection), a protein flanked by unique restriction enzymes (for easy exchange of the displayed protein) and a PDGFR (anchors proteins to the membrane). The proteins that we chose to express on the membrane are three different epitopes related to a specific disease (this part has specific epitopes that are known targets of multiple sclerosis). The addition of the TRE promoter allows inducible expression contingent upon the presence of doxycycline and a chosen activator protein (Tta or rTta).
Name | Type | Description | Designer | Length | |||
---|---|---|---|---|---|---|---|
BBa_K2520003 | Device | TRE-mono display:pro insulin-hGH | Noa Eden, Dana Kadosh | 1919 | |||
BBa_K2520004 | Device | TRE-mono display:MOG1-hGH | Noa Eden, Dana Kadosh | 1139 | |||
BBa_K2520005 | Device | TRE-mono display:MOG 3-hGH | Dana Kadosh, Noa Eden | 1106 | |||
BBa_K2520006 | Device | TRE-GFP-hGH | Noa Eden, Dana Kadosh, Maya Engal, Shir Ovadia | 1532 | |||
favorite | BBa_K2520007 | Device | TRE-Tri display-hGH | Dana Kadosh, Noa Eden | 2057 | ||
BBa_K2520008 | Device | CMV-Tri display-hGH | Dana Kadosh, Noa Eden | 2358 | |||
BBa_K2520009 | Device | CMV-tTA-hGH | Noa Eden, Dana Kadosh, Maya Engal, Shir Ovadia | 1860 | |||
BBa_K2520010 | Device | EF1a-Tri display-hGH | Dana Kadosh, Noa Eden | 2994 | |||
BBa_K2520011 | Device | EF1a-GFP-hGH | Dana Kadosh, Noa Eden | 2469 | |||
BBa_K2520012 | Device | EF1a promoter-mono pro insulin-hGH | Dana Kadosh, Noa Eden | 2856 | |||
BBa_K2520013 | Device | CMV-mono display:MOG 1-hGH | Noa Eden, Dana Kadosh | 1511 | |||
BBa_K2520014 | Device | CMV-mono display:MOG 3-hGH | Dana Kadosh, Noa Eden | 1407 | |||
BBa_K2520015 | Device | CMV-Mono Display:celiac 1-hGH | Dana Kadosh, Noa Eden | 1475 | |||
BBa_K2520016 | Device | CMV-Mono Display:celiac 3-hGHH | Dana Kadosh, Noa Eden | 1475 | |||
BBa_K2520017 | Device | CMV-mono Display:PLA 1-hGH | Noa Eden, Dana Kadosh | 1484 | |||
BBa_K2520018 | Device | CMV-Mono Display:PLA 3-hGH | Dana Kadosh, Noa Eden | 1502 | |||
BBa_K2520019 | Device | CMV-Mono Display:peanut 1-hGH | Dana Kadosh, Noa Eden | 1466 | |||
BBa_K2520020 | Device | CMV-Mono Display:peanut 3-hGH | Dana Kadosh, Noa Eden | 1466 |
![](https://static.igem.org/mediawiki/2016/5/5a/T--Technion_Israel--up_arrow.png)