Difference between revisions of "Team:MIT/protocols"
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<a href= “https://static.igem.org/mediawiki/2017/5/55/Culturing_%26_Splitting_Mammalian_Cells_%C2%B7_Benchling.pdf”>Culturing & Splitting HEK-293 Cells</a> | <a href= “https://static.igem.org/mediawiki/2017/5/55/Culturing_%26_Splitting_Mammalian_Cells_%C2%B7_Benchling.pdf”>Culturing & Splitting HEK-293 Cells</a> | ||
Revision as of 00:53, 29 October 2017
Protocols
Bacterial Lab
Transformation of E. coli Sequencing Sample Preparation for Flow Cytometry Resuspending Primers Resuspending_gBlocks Primer Design Pouring LB Agar Plates PCR Overnight liquid cultures Nanodrop Miniprep LR Reaction Golden Gate Assembly Glycerol Stock Gel Extraction Gel Extraction Diagnostic Restriction Digestion Annealing Oligonucleotides Pouring an Agarose GelTissue Culture
Culturing & Splitting HEK-293 Cells Culturing & Splitting NCI-H82 Cells DNA Transfection into HEK Cells Seeding Plates