Difference between revisions of "Team:MIT"

Line 4: Line 4:
 
<h1 style="color:#f20253; text-align: center; font-size: 40px; line-height: 40px;">Splice & Dice</h1>
 
<h1 style="color:#f20253; text-align: center; font-size: 40px; line-height: 40px;">Splice & Dice</h1>
  
−
<h2 style="color:#f20253; text-align: center; font-size: 20px; line-height: 40px;">Using alternative splicing to control alternative splicing in mammalian cells</h2>
+
<h2 style="color:#f20253; text-align: center; font-size: 20px; line-height: 40px;">Using dCas13a to control alternative splicing in mammalian cells</h2>
  
 
<p>Alternative splicing is a process that takes mRNA transcripts and modifies it in various ways to create a final mature mRNA molecule for translation. Some sequences known as introns are removed; other sequences known as exons remain to be translated. With such a process, a single gene can result in many types of proteins transcripts
 
<p>Alternative splicing is a process that takes mRNA transcripts and modifies it in various ways to create a final mature mRNA molecule for translation. Some sequences known as introns are removed; other sequences known as exons remain to be translated. With such a process, a single gene can result in many types of proteins transcripts

Revision as of 05:05, 29 October 2017

Splice & Dice

Using dCas13a to control alternative splicing in mammalian cells

Alternative splicing is a process that takes mRNA transcripts and modifies it in various ways to create a final mature mRNA molecule for translation. Some sequences known as introns are removed; other sequences known as exons remain to be translated. With such a process, a single gene can result in many types of proteins transcripts Our iGEM projects seeks to control alternative splicing of RNA, specifically exon skipping and inclusion, using a protein called Cas13a. This is a protein that attaches to RNA via a complementary guide RNA, then cuts the RNA strand. For our purposes, we're using a modified version of this protein, known as dCas13a, that can attach, but doesn't cut. By targeting certain portions of a fluorescent protein construct, we can determine whether or not we achieved the intended isoforms based on the presence or absence of the fluorescent protein in addition to sequencing.