Difference between revisions of "Team:Lethbridge/Improve"

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{{Team:Lethbridge/assets/reset.css}}
 
{{Team:Lethbridge/assets/reset.css}}
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{{Team:Lethbridge/assets/sidemenuremove.css}}
 
{{Team:Lethbridge/navbar}}
 
{{Team:Lethbridge/navbar}}
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{{Team:Lethbridge/assets/allpages.css}}
 
<html>
 
<html>
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<head>
 
 
<style>
 
<style>
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/*SIDE MENU FOR IGEM STUFF*/
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/*RESET AND REMOVAL CSS IS PART OF RESET AND SIDEMENUREMOVE TEMPLATE*/
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#sideMenu{width: 200px;position: absolute;top: 20px;left: 1020px;z-index: 10;padding-top: 0px;padding-bottom: 15px;padding-left: 15px;padding-right: 15px;background-color: white;text-align: left;display: none;z-index: 9996;}
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/* RULES FOR ALL PAGES ARE PART OF THE ALLPAGES.CSS TEMPLATE*/
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#content{width: 100%;padding: 0px;margin-left: 0px;}
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/* RULES FOR THIS PAGE ONLY */
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#top_title{overflow: hidden;display: none;}
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#top_menu_14{height: 20px;}
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#top_menu_under{height: 0px;}
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/*#globalWrapper{padding-bottom: 200px;}*/
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/*VISIBLE CONTENT STYLING BEGINS HERE*/
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body {padding-top: 3.89em; font-family: Arial, sans-serif; background-color:#F2F2F2; color: black !important;}
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.bannerImg {max-width: 100%; height:auto;}
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#allContent {margin-left: 5%; margin-right: 5%}
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h1, h2 {text-align: center; color: black !important}
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h3 {font-style: oblique;}
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.userWrapper {margin-left: 7%; margin-right: 7%;}
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.segmentHeader {font-size: 4vw; overflow: hidden; text-align: center;}
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.segmentHeader:before, .segmentHeader:after {background-color: black; content: ""; display: inline-block; height: 3px; position: relative; vertical-align: middle; width: 50%;}
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.segmentHeader:before {margin-left: -50%; right- 0.5 em;}
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.segmentHeader:after {left: 0.5em; margin-right: -50%;}
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.segmentDiv {margin-left: 5% !important; margin-right: 5% !important;}
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.pageText {font-size: calc(12px + 0.5vw) !important; line-height: calc(1.1em + 0.5vw) !important; text-align: left !important; width:99%; padding: 20px; font-weight: normal;}
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.segmentContainer p.segment2 {font-size: calc(22px + 0.5vw) !important; line-height: calc(1.1em + 0.5vw) !important;}
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.contentDiv {display: inline-block; vertical-align: middle;}
 
.contentDiv {display: inline-block; vertical-align: middle;}
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.dividerLine {margin-left: 20%; margin-right:20%; border: 1px solid darkgrey;}
 
  
 
</style>
 
</style>
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         <br><br><br><br>
 
         <br><br><br><br>
 
<h2 align= "left"> Methionine Synthetase (MetRS)</h2>
 
<h2 align= "left"> Methionine Synthetase (MetRS)</h2>
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<p class="pageText"> <b> Original part:</b> BBa_K567015
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<p class="text12"> <b> Original part:</b> BBa_K567015
 
<br> <b> Submitted by:</b>  SJTU-BioX-Shanghai 2011
 
<br> <b> Submitted by:</b>  SJTU-BioX-Shanghai 2011
 
<br> <b> Designed by:</b> You Wang
 
<br> <b> Designed by:</b> You Wang
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<h2 align= "left"> T7 RNA Polymerase (T7 RNAP)</h2>
 
<h2 align= "left"> T7 RNA Polymerase (T7 RNAP)</h2>
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<p class="pageText"> <b> Original part:</b> BBa_I2032
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<p class="text12"> <b> Original part:</b> BBa_I2032
 
<br><b> Submitted by:</b> MIT
 
<br><b> Submitted by:</b> MIT
 
<br> <b> Designed by:</b> Bartholomew Canton
 
<br> <b> Designed by:</b> Bartholomew Canton
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<h2 align= "left"> Elongation Factor Thermo Stable (EF-Ts)</h2>
 
<h2 align= "left"> Elongation Factor Thermo Stable (EF-Ts)</h2>
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<p class="pageText"> <b> Original part:</b> BBa_K1906000
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<p class="text12"> <b> Original part:</b> BBa_K1906000
 
<br>
 
<br>
 
<b> Submitted by:</b> XJTLU-China 2016
 
<b> Submitted by:</b> XJTLU-China 2016
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<h2 align= "left"> Elongation Factor Thermo Unstable (EF-Tu)</h2>
 
<h2 align= "left"> Elongation Factor Thermo Unstable (EF-Tu)</h2>
  
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<p class="pageText"> <b> Original part:</b> BBa_K1906004
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<p class="text12"> <b> Original part:</b> BBa_K1906004
 
<br>
 
<br>
 
  <b> Submitted by:</b> XJTLU-China 2016
 
  <b> Submitted by:</b> XJTLU-China 2016

Revision as of 18:35, 29 October 2017





Methionine Synthetase (MetRS)

Original part: BBa_K567015
Submitted by: SJTU-BioX-Shanghai 2011
Designed by: You Wang
Rational behind improvements: BBa_K567015 encodes for a truncated MetRS without the anticodon recognition domain. We have improved this part by including the entire coding sequence, which has been codon optimized for optimal expression in E. coli. For easy purification we have included a C- terminal 6 X His tag with a serine glycine linker. We have also optimized MetRS to be overexpressed in BL21 DE3 gold cell by putting it under the control of a T7 promoter (BBa_I719005), RBS (BBa_B0034) and double terminator (BBa_B0015).
Our Part:







T7 RNA Polymerase (T7 RNAP)

Original part: BBa_I2032
Submitted by: MIT
Designed by: Bartholomew Canton
Rational behind improvements: BBa_I2032 encodes exclusively for the coding region of T7 RNA polymerase. In order to improve it and incorporate it into our Next vivo system we have Codon optimized for use in E. coli and attached a N-terminal 6 X His tag with a serine glycine linker for easy purification. We have also optimized our T7 RNA polymerase to be overexpressed in BL21 DE3 gold cells by putting it under the control of a T7 promoter (BBa_I719005), RBS (BBa_B0034) and double terminator (BBa_B0015).
Our Part:







Elongation Factor Thermo Stable (EF-Ts)

Original part: BBa_K1906000
Submitted by: XJTLU-China 2016
Designed by: Wenbo Xu
Rational behind improvements:BBa_K1906000 encodes exclusively for the coding region of EF-Ts. In order to improve it and incorporate it into our Next vivo system we have codon optimized for use in E. coli and attached a C-terminal 6 X His tag with a serine glycine linker for easy purification. We have also optimized EF-Ts to be overexpressed in BL21 DE3 gold cells by putting it under the control of a T7 promoter (BBa_I719005), RBS (BBa_B0034) and double terminator (BBa_B0015). 
Our Part:







Elongation Factor Thermo Unstable (EF-Tu)

Original part: BBa_K1906004
Submitted by: XJTLU-China 2016
Designed by: Yuwei Han
Rational behind improvements:BBa_K1906004 encodes exclusively for the coding region of EF-Tu. In order to improve it and incorporate it into our Next vivo system we have codon optimized for use in E. coli and attached a N-terminal 6 X His tag with a serine glycine linker for easy purification. We have also optimized EF-Tu to be overexpressed in BL21 DE3 gold cells by putting it under the control of a T7 promoter (BBa_I719005), RBS (BBa_B0034) and double terminator (BBa_B0015). 
Our Part: