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− | <p class="text12">The N<i>ex</i>t <i>vivo</i> system is made up of >40 unique parts encoding both protein and RNA components. The main focus of our team this year was to submit all 38 TX-TL protein components to the registry as a <a href="https://2017.igem.org/wiki/index.php?title=Team:Lethbridge/Part_Collection">collection of parts</a> that anyone could use to produce their own N<i>ex</i>t <i>vivo</i> system. Several of these TX-TL components were already in the registry but were under different expression control, lacked purification tags, and/or were not codon optimized for expression in <i>E. coli</i>. As such we sought to <a href="https://2017.igem.org/Team:Lethbridge/Improve">improve these parts</a> for future use. | + | <p class="text12">The N<i>ex</i>t <i>vivo</i> system is made up of >40 unique parts encoding both protein and RNA components. The main focus of our team this year was to submit all 38 TX-TL protein components to the registry as a <a href="https://2017.igem.org/wiki/index.php?title=Team:Lethbridge/Part_Collection" id="pageLink">collection of parts</a> that anyone could use to produce their own N<i>ex</i>t <i>vivo</i> system. Several of these TX-TL components were already in the registry but were under different expression control, lacked purification tags, and/or were not codon optimized for expression in <i>E. coli</i>. As such we sought to <a href="https://2017.igem.org/Team:Lethbridge/Improve" id="pageLink">improve these parts</a> for future use. |
</p> | </p> | ||
− | <p class="text12">In addition to the TX-TL proteins, we also sought to BioBrick the ribosomal RNA genes and twenty tRNA genes. As a proof of concept for our novel tRNA purification design we only synthesized one representative tRNA gene before ordering them all. Additionally, the N<i>ex</i>t <i>vivo</i> system makes use of a previously submitted MS2BP part (<a href="http://parts.igem.org/Part:BBa_K2109108">BBA_K2109108 | + | <p class="text12">In addition to the TX-TL proteins, we also sought to BioBrick the ribosomal RNA genes and twenty tRNA genes. As a proof of concept for our novel tRNA purification design we only synthesized one representative tRNA gene before ordering them all. Additionally, the N<i>ex</i>t <i>vivo</i> system makes use of a previously submitted MS2BP part (<a href="http://parts.igem.org/Part:BBa_K2109108">BBA_K2109108 id="pageLink" />). |
</p> | </p> | ||
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<h2 class="segmentHeader">Part Collection</h2> | <h2 class="segmentHeader">Part Collection</h2> | ||
− | <p class="text12">The <a href="https://2017.igem.org/wiki/index.php?title=Team:Lethbridge/Part_Collection">N<i>ex</i>t <i>vivo</i> part collection</a> consists of the 38 TX-TL proteins required for RNA transcription and protein synthesis. Each part in the collection has a hexahistidine tag for simultaneous purification of all components, the T7 promoter (<a href="http://partsregistry.org/Part:BBa_I719005">BBa_I719005</a>), medium RBS (<a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034 | + | <p class="text12">The <a href="https://2017.igem.org/wiki/index.php?title=Team:Lethbridge/Part_Collection" id="pageLink">N<i>ex</i>t <i>vivo</i> part collection</a> consists of the 38 TX-TL proteins required for RNA transcription and protein synthesis. Each part in the collection has a hexahistidine tag for simultaneous purification of all components, the T7 promoter (<a href="http://partsregistry.org/Part:BBa_I719005">BBa_I719005</a>), medium RBS (<a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034 id="pageLink" />), and double terminator (<a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015 id="pageLink" />). |
</p> | </p> | ||
</div> | </div> | ||
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<div style="clear: both"></div> | <div style="clear: both"></div> | ||
− | <p class="text12">This year we designed <a href="https://2017.igem.org/wiki/index.php?title=Team:Lethbridge/Composite_Part">41 composite parts | + | <p class="text12">This year we designed <a href="https://2017.igem.org/wiki/index.php?title=Team:Lethbridge/Composite_Part">41 composite parts id="pageLink" />. Of the 41 parts, it contains 38 proteins essential for prokaryotic transcription and translation (TX-TL) as well 3 additional constructs for tRNA purification and TX-TL validation.</p> |
<div style="clear: both"></div> | <div style="clear: both"></div> | ||
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<p>Basic parts paragraph.</p> | <p>Basic parts paragraph.</p> | ||
− | <p><a href="https://2017.igem.org/wiki/index.php?title=Team:Lethbridge/Improve">Improved parts table</a></p> </div> | + | <p><a href="https://2017.igem.org/wiki/index.php?title=Team:Lethbridge/Improve" id="pageLink">Improved parts table</a></p> </div> |
</div> | </div> | ||
<div style="clear: both"></div> | <div style="clear: both"></div> | ||
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<p>All basic parts pages created on the registry of standard biological parts. Contains all 38 proteins essential for prokaryotic transcription and translation (Tx/Tl) as well as the parts for our tRNA purification and Tx/Tl validation construct.</p> | <p>All basic parts pages created on the registry of standard biological parts. Contains all 38 proteins essential for prokaryotic transcription and translation (Tx/Tl) as well as the parts for our tRNA purification and Tx/Tl validation construct.</p> | ||
− | <p><a href="https://2017.igem.org/wiki/index.php?title=Team:Lethbridge/Basic_Part">Basic parts table</a> | + | <p><a href="https://2017.igem.org/wiki/index.php?title=Team:Lethbridge/Basic_Part" id="pageLink">Basic parts table</a> |
</div> | </div> |
Revision as of 20:41, 29 October 2017
![](https://static.igem.org/mediawiki/2017/6/64/Banner_PAoverview.png)
The Next vivo system is made up of >40 unique parts encoding both protein and RNA components. The main focus of our team this year was to submit all 38 TX-TL protein components to the registry as a collection of parts that anyone could use to produce their own Next vivo system. Several of these TX-TL components were already in the registry but were under different expression control, lacked purification tags, and/or were not codon optimized for expression in E. coli. As such we sought to improve these parts for future use.
In addition to the TX-TL proteins, we also sought to BioBrick the ribosomal RNA genes and twenty tRNA genes. As a proof of concept for our novel tRNA purification design we only synthesized one representative tRNA gene before ordering them all. Additionally, the Next vivo system makes use of a previously submitted MS2BP part (BBA_K2109108 id="pageLink" />).
Part Collection
The Next vivo part collection consists of the 38 TX-TL proteins required for RNA transcription and protein synthesis. Each part in the collection has a hexahistidine tag for simultaneous purification of all components, the T7 promoter (BBa_I719005), medium RBS (BBa_B0034 id="pageLink" />), and double terminator (BBa_B0015 id="pageLink" />).
Composite Parts
General Protein Construct
![](https://static.igem.org/mediawiki/2017/9/97/T--Lethbridge--generalconstruct.png)
General RNA Construct
![](https://static.igem.org/mediawiki/2017/a/a8/T--Lethbridge--RNAconstruct.png)
Basic Parts
All basic parts pages created on the registry of standard biological parts. Contains all 38 proteins essential for prokaryotic transcription and translation (Tx/Tl) as well as the parts for our tRNA purification and Tx/Tl validation construct.
![](https://static.igem.org/mediawiki/2017/7/7d/Banner_footer_blank.png)