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− | Human practice activities are integrated throughout the design and execution of our project. Interaction with the public and fellow researchers led to many improvements in our design, like the design of behavior experiment, the improvement in microinjection procedures and better C. elegans mating method.</font> | + | Human practice activities are integrated throughout the design and execution of our project. Interaction with the public and fellow researchers led to many improvements in our design, like the design of behavior experiment, the improvement in microinjection procedures and better <i>C. elegans</i> mating method.</font> |
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== Collaboration with Fellow Researchers== | == Collaboration with Fellow Researchers== | ||
− | At initial stage, we faced lots of problems on microinjection, such as low success rate and low male worms rate after mating. So we contacted a laboratory which research mainly in C.elegans area in HKBU(Hong Kong Baptist University) with the help of Prof.Huang. We communicated with Doctor Ding on microinjection issues in the lab. With their kind instruction on our microinjection methods, we got our MiniMos part successfully inserted into C.elegans. | + | At initial stage, we faced lots of problems on microinjection, such as low success rate and low male worms rate after mating. So we contacted a laboratory which research mainly in <i>C. elegans</i> area in HKBU(Hong Kong Baptist University) with the help of Prof.Huang. We communicated with Doctor Ding on microinjection issues in the lab. With their kind instruction on our microinjection methods, we got our MiniMos part successfully inserted into <i>C. elegans</i>. |
{{SUSTech_Image_Center_8 | filename=T--SUSTech_Shenzhen--HP-silver-11.png|width=6600px|caption=<B> 11: Microinjection Discussion in HKBU</B>}} | {{SUSTech_Image_Center_8 | filename=T--SUSTech_Shenzhen--HP-silver-11.png|width=6600px|caption=<B> 11: Microinjection Discussion in HKBU</B>}} |
Revision as of 11:25, 30 October 2017
Human Practices
Our step to make a better world
Contents
Human practice activities are integrated throughout the design and execution of our project. Interaction with the public and fellow researchers led to many improvements in our design, like the design of behavior experiment, the improvement in microinjection procedures and better C. elegans mating method.
Exchange of Ideas with Fellow Scholars
Considering our needs to design the behavioral experiment to train worms, we held a seminar with behavioristic psychology professor Peng Li of Shenzhen University, aiming to get some inspirations for our behavior experiment design.
In the seminar, Prof. Li gave some professional advise on our design in aspect of behavioristic psychology. Professor pointed out the lacking of an indifferent stimulus on which we can build a new behavior. He suggested building tropism on an indifferent factor by training worms with favorite/abhorrent stimulus. That was much easier than training worms to change their pre-existing likes and dislikes. The advice we got from Prof. Li helped us correct our principles and guided us to design our new behavior experiment.
With the advice of Prof. Li, we amended our behavioral experiment design, preventing ourselves from wasting time.
Communication with Other Teams
During 26th,Aug and 28th,Aug this year, our team was invited to attend CCiC (Conference of China iGEMer Committee) in Fuzhou, China, to present our project among peers researchers.
All the 49 teams invited to the conference gave presentations and exhibited posters about their projects. During the conference, especially in the poster session, we had many opportunities to communicate with other teams and exchange ideas of our project designs.
After presenting our project on the conference, we had a short discussion with the judges, who provided us with much valuable advice. Judges raised concerns that the long-time training which may make C. elegans too big.
Therefore, we verified this hypothesis and modified our training pattern and experiment design.
In this conference, we were enlightened about the design of our experiment. This opportunity also provided us with new ideas about synthetic biology.
Collaboration with Fellow Researchers
At initial stage, we faced lots of problems on microinjection, such as low success rate and low male worms rate after mating. So we contacted a laboratory which research mainly in C. elegans area in HKBU(Hong Kong Baptist University) with the help of Prof.Huang. We communicated with Doctor Ding on microinjection issues in the lab. With their kind instruction on our microinjection methods, we got our MiniMos part successfully inserted into C. elegans.