Difference between revisions of "Team:Austin UTexas/Improve"

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<h1>Improve</h1>
 
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<p>For teams seeking to improve upon a previous part or project, you should document all of your work on this page. Please remember to include all part measurement and characterization data on the part page on the Regisrty. Please include a link to your improved part on this page.</p>
 
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<h3>Gold Medal Criterion #2</h3>
 
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<p><b>Standard Tracks:</b> Improve the function of an existing BioBrick Part. The original part must NOT be from your 2017 part number range. If you change the original part sequence, you must submit a new part. In addition, both the new and original part pages must reference each other. This working part must be different from the part documented in bronze #4 and silver #1.
 
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<b>Special Tracks:</b> Improve the function of an existing iGEM project (that your current team did not originally create) and display your achievement on your wiki.</p>
 
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<h2 style="font-family: verdana">The Improved Blue Chromoprotein</h2>
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<p style="font-family: verdana">Chromoproteins are known to be unstable, meaning that they create a high metabolic burden on the cell that they are in. In order to combat this metabolic burden, a technique called codon optimization was used. Codon optimization involves exchanging certain codons for ones that have known to be more translationally efficient in a certain species while retaining the same sequence of amino acids. Making translation more efficient, in turn, lowers the metabolic burden on the E.coli that the gene is present in. Therefore codon optimization greatly improves the function of the original blue chromoprotein (BBa_K592009). The codon optimized sequence has been transformed into a plasmid with the pSB1C3 backbone with the K608002 promoter and RBS sequence and also has been sequence verified to be the correct nucleotide sequence. Another improvement of this part is that the miniprepped sequence now has the promoter and RBS sequence attached to it, which can be taken and transformed immediately instead of having to go through the process of digestion and ligation all over again to add different parts to it.</p>
  
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<p style="font-family: verdana">The codon optimized blue chromoprotein composite part can be found on the iGEM registry as: <a href="http://parts.igem.org/Part:BBa_K2253002">BBa_K2253002</a></b>.
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Revision as of 17:05, 30 October 2017