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− | Additionally, in our project we used the existing tTA transactivator part (<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1061015">BBa_K1061015</a>) to test our | + | Additionally, in our project we used the existing tTA transactivator part (<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1061015">BBa_K1061015</a>) to test our <a href="https://2017.igem.org/Team:TECHNION-ISRAEL/delay_mechanism" >Delay Mechanism</a>. To use this basic part, we had to clone it into a mammalian expression vector, requiring time and effort. <br>In order to save future teams time, we have improved this part (<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2520009">BBa_K2520009</a>) by adding a promoter (CMV) and a terminator (hGH poly A). Additionally, we tested this part and characterized it. |
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Revision as of 17:55, 30 October 2017
Parts Improvement
![](https://static.igem.org/mediawiki/2017/e/e1/T--TECHNION-ISRAEL--improve_part.png)
We have improved two existing parts in an attempt to increase their utility to the iGEM community.
We improved the existing part (BBa_K1119008) - a device containing the GFP reporter gene under the CMV promoter with an hGH poly A terminator. By replacing the CMV promoter with TRE promoter (BBa_K2520006) we have created a convenient and important positive control for TRE based inducible systems.
Additionally, in our project we used the existing tTA transactivator part (BBa_K1061015) to test our Delay Mechanism. To use this basic part, we had to clone it into a mammalian expression vector, requiring time and effort.
In order to save future teams time, we have improved this part (BBa_K2520009) by adding a promoter (CMV) and a terminator (hGH poly A). Additionally, we tested this part and characterized it.
Name | Type | Description | Designer | Length | |||
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BBa_K2520006 | Device | TRE-GFP-hGH | Noa Eden, Dana Kadosh, Maya Engal, Shir Ovadia | 1532 | |||
BBa_K2520009 | Device | CMV-tTA-hGH | Noa Eden, Dana Kadosh, Maya Engal, Shir Ovadia | 1860 |
![](https://static.igem.org/mediawiki/2016/5/5a/T--Technion_Israel--up_arrow.png)