Difference between revisions of "Team:Manchester/Improve"

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<p style="text-align: center; font-size: 20px!important"><i> Please visit our <a href="https://2017.igem.org/Team:Manchester/Part_Collection"><b>Part Collection</b></a> page for ALL PARTS.</i></p>
 
<p style="text-align: center; font-size: 20px!important"><i> Please visit our <a href="https://2017.igem.org/Team:Manchester/Part_Collection"><b>Part Collection</b></a> page for ALL PARTS.</i></p>
  
<p style="text-align: center; font-size: 20px!important"> The Manchester iGEM 2017 team have improved part <a href="http://partsregistry.org/Part:BBa_K562001"><b>BBa_K562001</b></a>, originally submitted by team <a href="https://2011.igem.org/Team:Dundee"></b>Dundee 2011</b></a>.</p>
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<p style="text-align: center; font-size: 18px!important"> The Manchester iGEM 2017 team have improved part <a href="http://partsregistry.org/Part:BBa_K562001"><b>BBa_K562001</b></a>, originally submitted by team <a href="https://2011.igem.org/Team:Dundee"></b>Dundee 2011</b></a>.</p>
  
 
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<p style="text-align: center; font-size: 20px!important">We have removed the illegal XbaI site at 179 in BBa_K562001 to make it biobrick compatible. </p>
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<p style="text-align: center; font-size: 18px!important">We have removed the illegal XbaI site at 179 in BBa_K562001 to make it biobrick compatible. </p>
 
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<p style="text-align: center; font-size: 20px!important">We have also further improved this part by adding different Anderson promoters (<a href="http://parts.igem.org/Part:BBa_J23105">Low BBa_J23105</a>, <a href="http://parts.igem.org/Part:BBa_J23108">Medium BBa_J23108</a>, <a href="http://parts.igem.org/Part:BBa_J23104">High BBa_J23104</a>) to control the expression level of the localisation tag. This enables optimisation of localisation for specific purposes. For example, when targeting a particularly large protein, it may be advantageous to use a lowly expressing promoter to prevent any disturbance to micro-compartment formation. </p>
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<p style="text-align: center; font-size: 18px!important">We have also further improved this part by adding different Anderson promoters (<a href="http://parts.igem.org/Part:BBa_J23105">Low BBa_J23105</a>, <a href="http://parts.igem.org/Part:BBa_J23108">Medium BBa_J23108</a>, <a href="http://parts.igem.org/Part:BBa_J23104">High BBa_J23104</a>) to control the expression level of the localisation tag. This enables optimisation of localisation for specific purposes. For example, when targeting a particularly large protein, it may be advantageous to use a lowly expressing promoter to prevent any disturbance to micro-compartment formation. </p>
 
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<table style="width: 1000px; max-width: 95vw">
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<table class="table table-bordered table-striped" style="width: 1000px; max-width: 95vw">
  
 
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Revision as of 20:24, 30 October 2017

Part Collection



f


Please visit our Part Collection page for ALL PARTS.

The Manchester iGEM 2017 team have improved part BBa_K562001, originally submitted by team Dundee 2011.


We have removed the illegal XbaI site at 179 in BBa_K562001 to make it biobrick compatible.


We have also further improved this part by adding different Anderson promoters (Low BBa_J23105, Medium BBa_J23108, High BBa_J23104) to control the expression level of the localisation tag. This enables optimisation of localisation for specific purposes. For example, when targeting a particularly large protein, it may be advantageous to use a lowly expressing promoter to prevent any disturbance to micro-compartment formation.


Part Name BioBrick Description Part Type
LowPromoter_PduD(1-20)_mCherry BBa_K2213006 BBa_K562001 with the illegal XbaI site removed, and with a low promoter (BBa_J23105), and mCherry added. Basic - Improved
MediumPromoter_PduD(1-20)_mCherry BBa_K2213007 BBa_K562001 with the illegal XbaI site removed, and with a medium promoter (BBa_J23108), and mCherry added. Basic - Improved
HighPromoter_PduD(1-20)_mCherry BBa_K2213008 BBa_K562001 with the illegal XbaI site removed, and with a high promoter (BBa_J23104), and mCherry added. Basic - Improved