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Revision as of 01:48, 31 October 2017
![](https://static.igem.org/mediawiki/2017/b/b1/Banner_PAcollection.png)
Part Collection
![](https://static.igem.org/mediawiki/2017/f/f4/T--Lethbridge--partcollection.png)
The Next vivo system is made up of >40 unique parts encoding both protein and RNA components. The main focus of our team this year was to submit all 38 TX-TL protein components to the registry as a collection of parts that anyone could use to produce their own Next vivo system. Several of these TX-TL components were already in the registry but were under different expression control, lacked purification tags, and/or were not codon optimized for expression in E. coli. As such we sought to improve these parts for future use.
Part Design Overview
![](https://static.igem.org/mediawiki/2017/9/97/T--Lethbridge--generalconstruct.png)
Each part in our collection was designed with several features in mind:
- Optimized for expression in E. coli
- N- or C-terminal hexahistidine affinity tag
- Constructs contain the T7 promoter (BBa_I719005), medium RBS (BBa_B0034), and double terminator (BBa_B0015)