Difference between revisions of "Team:Lethbridge/Part Collection"

The Next vivo system is made up of >40 unique parts encoding both protein and RNA components. The main focus of our team this year was to submit all 38 TX-TL protein components to the registry as a collection of parts that anyone could use to produce their own Next vivo system. Several of these TX-TL components were already in the registry but were under different expression control, lacked purification tags, and/or were not codon optimized for expression in E. coli. As such we sought to improve these parts for future use.

Part Design Overview

Each part in our collection was designed with several features in mind:

• Optimized for expression in E. coli
• N- or C-terminal hexahistidine affinity tag
• Constructs contain the T7 promoter (BBa_I719005), medium RBS (BBa_B0034), and double terminator (BBa_B0015)

Future Use for iGEM

Our hopes are that going forward, teams will be able to use our Next vivo system in the design of their projects. Whether they require a cell-free system to run constructs or just want to test constructs outside of their host organism, Next vivo can be that tool! Designing the system to be purified easily and open-source was key design feature to make this technology available to anyone who wants a cell-free system.

We envision Next vivo continually improving through the addition of new modules and features that can expand the functionalities of the system. These range from accepting non-standard amino acids, thermostability, and difficult protein expression modules.

Our Parts in the Registry