Difference between revisions of "Team:Manchester/Improve"

We have also further improved this part by adding different Anderson promoters (Low BBa_J23105, Medium BBa_J23108, High BBa_J23104) to control the expression level of the localisation tag.

This enables optimisation of localisation for specific purposes. For example, when targeting a particularly large protein, it may be advantageous to use a lowly expressing promoter to prevent any disturbance to micro-compartment formation.

Part Name	BioBrick	Description	Part Type
LowPromoter_PduD(1-20)_mCherry	BBa_K2213006	BBa_K562001,with the illegal XbaI site removed, and with a low promoter (BBa_J23105), and mCherry added.	Basic - Improved
MediumPromoter_PduD(1-20)_mCherry	BBa_K2213007	BBa_K562001, with the illegal XbaI site removed, and with a medium promoter (BBa_J23108), and mCherry added.	Basic - Improved
HighPromoter_PduD(1-20)_mCherry	BBa_K2213008	BBa_K562001, with the illegal XbaI site removed, and with a high promoter (BBa_J23104), and mCherry added.	Basic - Improved

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 <p style="text-align: center; font-size: 20px!important"><i> Please visit our <a href="https://2017.igem.org/Team:Manchester/Part_Collection"><b>Part Collection</b></a> page for ALL PARTS.</i></p>
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-<p style="text-align: center; font-size: 18px!important"> The Manchester iGEM 2017 team have improved part <a href="http://partsregistry.org/Part:BBa_K562001"><b>BBa_K562001</b></a>, originally submitted by team <a href="https://2011.igem.org/Team:Dundee"></b>Dundee 2011</b></a>.</p>
+<p style="text-align: center; font-size: 18px!important"> The Manchester iGEM 2017 team have improved part <a href="http://parts.igem.org/Part:BBa_K562001"><b>BBa_K562001</b></a>, originally submitted by team <a href="https://2011.igem.org/Team:Dundee"></b>Dundee 2011</b></a>.</p>
 <p style="text-align: center; font-size: 18px!important">We have removed the illegal XbaI site at 179 in BBa_K562001 to make it biobrick compatible. </p>