Difference between revisions of "Team:TECHNION-ISRAEL/Diseases"

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title="Celiac is a long term autoimmune disorder affecting  the internal intestine that is caused due to T cells attacking the Gliadin which is absorbed in the inner wall cells of the small intestine. Gliadin is one of a two proteins that forms gluten. This process leads to severe inflammation, and thus interferes with the absorption of food and greatly increases the chances of contracting colorectal cancer. The prevalence of celiac disease is approximately 0.5% -1% in different parts of the world [4].
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title="Celiac is a long term autoimmune disorder affecting  the internal intestine that is caused due to T cells attacking the Gliadin which is absorbed in the inner wall cells of the small intestine. Gliadin is one of a two proteins that forms gluten. This process leads to severe inflammation, and thus interferes with the absorption of food and greatly increases the chances of contracting colorectal cancer. The prevalence of celiac disease is approximately 0.5-1% in different parts of the world [4].
 
The only treatment that eliminates all symptoms (but does not cure the disease) is a strict avoidance of foods containing gluten."
 
The only treatment that eliminates all symptoms (but does not cure the disease) is a strict avoidance of foods containing gluten."
 
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title="Diabetes type 1 is a chronic disease of the pancreas that is caused due to T cells attacking the insulin producing beta cells, within the pancreas. Globally, nearly 40 million people [3] suffer from type I diabetes. This disease leads pancreatic dysfunction and insulin dependence."
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title="Diabetes type 1 is a chronic disease of the pancreas that is caused due to T cells attacking the insulin producing beta cells, within the pancreas. Globally, nearly 40 million people suffer from type I diabetes. This disease leads pancreatic dysfunction and insulin dependence [3]."
 
alt="type1">
 
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Revision as of 21:24, 1 November 2017

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Modular Tri-Display plasmid

Diseases & Epitopes



There are hundreds of autoimmune and allergic diseases. They have a tremendous effect on people’s lives, and exact a heavy financial and emotional cost. In our project, we chose to focus on five specific diseases. We chose these diseases because they are familiar and very different in their pathophysiology, thus demonstrating both the pertinence, and modularity, of our proposed treatment.

click on the different parts in the image

bee peanut MS Celiac type1



Epitopes

An Epitope is the part of an antigen that is recognized by the immune system, specifically by antibodies, B cells, or T cells.


Allergies /
autoimmune diseases 		Epitopes Amino acids sequences
Multiple sclerosis Domain 1 in the MOG - myelin oligodendrocyte protein GQFRVIGPGHPIRALVGDEAE [9]
Domain 2 in the MOG - myelin oligodendrocyte protein MEVGMYRSPFSRVVHLYRNGK [9]
Domain 3 in the MOG - myelin oligodendrocyte protein SYQEEAAVELKVED [9]
Diabetes type 1 Proinsulin MALWMRLLPL LALLALWGPD PAAAFVNQHLCGSHLVEALY LVCGERGFFYTPKTRREAED LQVGQVELGG GPGAGSLQPL ALEGSLQKRG IVEQCCTSIC SLYQLENYCN [10]
Celiac Domain 1 in the 33 mer peptide PFPQPQLPY [11] [12]
Domain 2 in the 33 mer peptide PQPQLPYPQ [11] [12]
Domain 3 in the 33 mer peptide PYPQPQLPY [11] [12]
Peanut allergy Overlapping domain 1 between Ara-h2 and Ara-h6 proteins KRELRN [13] [14]
Overlapping domain 2 between Ara-h2 and Ara-h6 proteins LQGRQQ [13] [14]
Overlapping domain 3 between Ara-h2 and Ara-h6 proteins DPYSPS [13] [14]
Bee venom allergy Domain 1 in the phospholipase A2 (PLA) protein CLHYTVDKSKPK [15]
Domain 2 in the phospholipase A2 (PLA) protein YFVGKMYFNLID [15]
Domain 3 in the phospholipase A2 (PLA) protein ESKHGLTNTASHTRLSCD [15]



  1. Dilokthornsakul, Piyameth, et al. "Multiple sclerosis prevalence in the United States commercially insured population." Neurology 86.11 (2016): 1014-1021.‏
  2. Fletcher, J. M., et al. "T cells in multiple sclerosis and experimental autoimmune encephalomyelitis." Clinical & Experimental Immunology 162.1 (2010): 1-11.‏
  3. Reuters, Thomson. “Diabetes cases soar to record 382 million worldwide.” CBCnews, CBC/Radio Canada, 14 Nov. 2013, www.cbc.ca/news/health/diabetes-cases-hit-record-382-million-worldwide-1.2426381.
  4. Gujral, Naiyana, Hugh J. Freeman, and Alan BR Thomson. "Celiac disease: prevalence, diagnosis, pathogenesis and treatment." World journal of gastroenterology: WJG 18.42 (2012): 6036.‏
  5. Al-Muhsen, Saleh, Ann E. Clarke, and Rhoda S. Kagan. "Peanut allergy: an overview." Canadian Medical Association Journal 168.10 (2003): 1279-1285.
  6. Mueller, Geoffrey A., Soheila J. Maleki, and Lars C. Pedersen. "The molecular basis of peanut allergy." Current allergy and asthma reports 14.5 (2014): 1-9.‏
  7. Ewan, Pamela W. "ABC of allergies: venom allergy." BMJ: British Medical Journal 316.7141 (1998): 1365.
  8. Müller, U., et al. "Increased specificity of diagnostic tests with recombinant major bee venom allergen phospholipase A2." Clinical & Experimental Allergy 27.8 (1997): 915-920.‏
  9. Mendel, Itzhack, Nicole Kerlero de Rosbo, and Avraham Ben‐Nun. "A myelin oligodendrocyte glycoprotein peptide induces typical chronic experimental autoimmune encephalomyelitis in H-2b mice: Fine specificity and T cell receptor Vβ expression of encephalitogenic T cells." European journal of immunology 25.7 (1995): 1951-1959.‏
  10. Narendran, Parth, Stuart I. Mannering, and Leonard C. Harrison. "Proinsulin—a pathogenic autoantigen in type 1 diabetes." Autoimmunity reviews 2.4 (2003): 204-210
  11. Ozuna, Carmen V., et al. "Diversification of the celiac disease α‐gliadin complex in wheat: a 33‐mer peptide with six overlapping epitopes, evolved following polyploidization." The Plant Journal 82.5 (2015): 794-805
  12. Schalk, Kathrin, et al. "Quantitation of the immunodominant 33-mer peptide from α-gliadin in wheat flours by liquid chromatography tandem mass spectrometry." Scientific Reports 7 (2017): 45092.
  13. Mishra, Ankita, Anuja Jain, and Naveen Arora. "Mapping B‐cell epitopes of major and minor peanut allergens and identifying residues contributing to IgE binding." Journal of the science of food and agriculture 96.2 (2016): 539-547.
  14. Otsu, K., R. Guo, and S. C. Dreskin. "Epitope analysis of Ara h 2 and Ara h 6: characteristic patterns of IgE‐binding fingerprints among individuals with similar clinical histories." Clinical & Experimental Allergy 45.2 (2015): 471-484.
  15. Carballido, J. M., et al. "T cell epitope specificity in human allergic andnonallergic subjects to bee venom phospholipase A2." The Journal of Immunology 150.8 (1993): 3582-3591
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Department of Biotechnology & Food Engineering
Technion – Israel Institute of Technology
Haifa 32000, Israel

    • igem.technion.2017@gmail.com