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Revision as of 03:15, 2 November 2017

Experiments with Small Cell Lung Cancer

Once we had decided on a disease model, namely Small Cell Lung Cancer, we needed an in-vivo way of testing our system. To this end we obtained the H82 Cell line from ATCC.

Preliminary Results

The first thing we had to ensure was that we were able to get DNA into the new cell line. We performed a Viafect optimization to test what our ratio of plasmid to transfection reagent should be. We transfected constitutively expressed BFP and reagent at ratios 1:1, 1:2, 1:3 and 1:4.

Viafect Optimization

Ratio of Viafect:BFP vs. the blue fluorescent output (AU) of the H82s

We see the most fluorescent output at a 1:3 ratio of Viafect:BFP. Moving forward, we transfected at that ratio.