Difference between revisions of "Team:KU Leuven"

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<h1>The iGEM KULeuven 2017 Team presents:</h1><p><strong>HEKcite!</strong> Inspired by the human heart rhythm, we aim to create an electrophysiological oscillator from eukaryotic cells. Rhythmic contraction of heart cells is coordinated by a small group of cells called the sinus node that have an intrinsic frequency of de- and repolarization. This frequency of electrical oscillation is influenced by environmental parameters as well as certain molecular substrates. The oscillator that is proposed here consists of genetically modified excitable Human Embryonic Kidney (HEK) cells, altered to contain the intrinsic pacemaker ability found in sinus cells. As witnessed in heart cells, the rhythm would be dependent on substrate-activated ion channels in the membrane. As there is a great variety of ion channels available in nature, the oscillator could be modified to measure concentrations of many specific substrates. By integrating a certain ion channel into the oscillating system, specificity for a substrate can be chosen.Building an electrical oscillator from cells has several advantages. Extra- or intracellular changes that influence the conductance of ion channels in the membrane have an immediate impact on the frequency of oscillation. Once these cells are connected to each other (by for example gap-junctions), they generate an electrical signal that can easily be measured from a distance and non-invasively—similar to the way electrocardiography (ECG) and electroencephalography (EEG) measure electrical activity in the heart and brain respectively.
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A multi-purpose sensor suitable for this system could be developed for medical and biotechnological applications. One such application is the measurement of drugs that interact with ion channels, such as anti-epileptics or a certain class of immunosuppressants.</p>
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<h2>Description</h2>
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<h2>Education</h2>
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<h2>Human Practices</h2>
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<div class="game">
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<h2>Game</h2>
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Revision as of 14:34, 24 July 2017

Description

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Education

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Human Practices

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Game

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