Team:HK SKHLPSS/Results
Contents
Results
Gel Electrophoresis
This graph is our result. We have done this test so that we can see the size of DNA strands. We can see that if the DNA strand move slower, it has a larger size. If the DNA strand move faster, it has a smaller size. We also can see that on lane 10, which included all DNA strands, is staying highest. It shows that our design of nano-cube worked and they are successful to integrate because it moves slowly and the position is high.
DNA Peroxidase Assay Result
These two graphs are our results. In the first graph, it shows that the nano-cube gave a very high signal of absorbance at 0.85 on its own .The presence of target shows a low signal at 0.75. Then, we conduct another experiment with different concentrations of target. The second graph shows the relationship between the reduction of the absorbance and the concentration of the target strand. We found that the higher the concentration of the target was, the lower the signal was. Reduction in absorbance is directly proportional to the concentration of target showing that the presence of the target actually reduced the signal! Although it is out of our expectation, it’s suggesting that we have accidentally discovered its signal off ability. We deduced the cube is very flexible and will easily be closed by the split quadruplex sequences and we deduced that the presence of target might just prohibit the cube to close.
mRNA Peroxidase Assay Result
Cloning
