Team:Lethbridge/Improve

Improved Parts

BBa_K2443037 - T7 RNA Polymerase

Original part: BBa_I2032

Submitted by: MIT in 2006

designed by: Bartholomew Canton

Rational behind improvements:

BBa_I2032 encodes exclusively for the coding region of T7 RNA polymerase. In order to improve it and incorporate it into our NextVivo system we have Codon optimized for use in Escherichia coli and attached a N-terminal 6 X His tag with a serine glycine linker for easy purification. We have also optimized our T7 RNA polymerase to be overexpressed in BL21DE3 gold cells by putting it under the control of a T7 promoter (BBa_I719005), RBS (BBa_B0034) and double terminator (BBa_B0015)

BBa_K2443037 - T7 RNA Polymerase

Original part: BBa_I2032

Submitted by: MIT in 2006

designed by: Bartholomew Canton

Rational behind improvements:

BBa_I2032 encodes exclusively for the coding region of T7 RNA polymerase. In order to improve it and incorporate it into our NextVivo system we have Codon optimized for use in Escherichia coli and attached a N-terminal 6 X His tag with a serine glycine linker for easy purification. We have also optimized our T7 RNA polymerase to be overexpressed in BL21DE3 gold cells by putting it under the control of a T7 promoter (BBa_I719005), RBS (BBa_B0034) and double terminator (BBa_B0015)